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IgG antibody

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Anti Mouse IgG (Rat Adsorbed) Ab - DyLight thumbnail image 1 Anti Mouse IgG (Rat Adsorbed) Ab - DyLight thumbnail image 2 Anti Mouse IgG (Rat Adsorbed) Ab - DyLight thumbnail image 3 Anti Mouse IgG (Rat Adsorbed) Ab - DyLight thumbnail image 4 Anti Mouse IgG (Rat Adsorbed) Ab - DyLight thumbnail image 5 Anti Mouse IgG (Rat Adsorbed) Ab - DyLight thumbnail image 6
Anti Mouse IgG (Rat Adsorbed) Ab - DyLight gallery image 1
Figure A. FITC conjugated Mouse anti Canine CD45 (MCA1042F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). Figure B. FITC conjugated Mouse anti Canine CD45 (MCA1042F) and purified Mouse anti Canine CD11b (MCA1777S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Anti Mouse IgG (Rat Adsorbed) Ab - DyLight gallery image 2
Figure A. PE conjugated Mouse anti Canine CD45 (MCA1042PE) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). Figure B. FITC conjugated Mouse anti Canine CD45 (MCA1042F) and purified Mouse anti Canine CD11c (MCA1778S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). All experiments performed on red cell lysed canine blood gated on monocytes.
Anti Mouse IgG (Rat Adsorbed) Ab - DyLight gallery image 3
Figure A. A488 conjugated Mouse anti Canine CD4 (MCA1038A488) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). Figure B. A488 conjugated Mouse anti Canine CD4 (MCA1038A488) and purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Anti Mouse IgG (Rat Adsorbed) Ab - DyLight gallery image 4
Figure A. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649) and Rat IgG2b FITC isotype control (MCA6006F). Figure B. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649) and Rat anti Canine CD4 FITC (MCA1038F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Anti Mouse IgG (Rat Adsorbed) Ab - DyLight gallery image 5
Figure A. FITC conjugated Mouse anti Canine CD8 (MCA1039F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). Figure B. FITC conjugated Mouse anti Canine CD8 (MCA1039F) and purified Mouse anti Canine CD45RA (MCA2036S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Anti Mouse IgG (Rat Adsorbed) Ab - DyLight gallery image 6
Figure A. Purified Mouse anti Canine CD45RA (MCA2036S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649) and Rat IgG1 FITC isotype control (MCA6004F). Figure B. Purified Mouse anti Canine CD45RA (MCA2036S) detected with Goat anti Mouse IgG1 PE (STAR74D649) and Rat anti Canine CD8 FITC (MCA1039F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Goat anti Mouse IgG (Rat Adsorbed)

Goat anti Mouse IgG:DyLight®488 (Rat Adsorbed)

Goat anti Mouse IgG:Dylight®800 (Rat Adsorbed)

Goat anti Mouse IgG:Dylight®405 (Rat Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
STAR74
Datasheet
E F
MSDS
0.5 mg loader

STAR74D488GA
Datasheet
F IF
MSDS
0.1 mg loader

STAR74D800GA
Datasheet
F IF WB
MSDS
0.1 mg loader

STAR74D405GA
Datasheet
F IF
MSDS
0.1 mg loader

Goat anti Mouse IgG (Rat Adsorbed) antibody recognizes mouse IgG. Cross-reactivity with rat IgG has been minimised by adsorption.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to DyLight®488 - liquid
Product Form
Purified IgG conjugated to DyLight®800 - liquid
Product Form
Purified IgG conjugated to DyLight®405 - liquid
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Mouse IgG.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch
Guarantee
18 months from date of despatch
Guarantee
18 months from date of despatch.

More Information

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GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Flow Cytometry 1/100 1/200
Immunofluorescence
Flow Cytometry
Immunofluorescence
Western Blotting 1/5000 1/20000
Flow Cytometry 1/25 1/200
Immunofluorescence
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Product Specific References

References for IgG antibody

  1. Jarrett, B.R. et al. (2010) In vivo mapping of vascular inflammation using multimodal imaging.
    PLoS One. 5(10). pii: e13254.
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