IgG antibody
HRP conjugated Rabbit anti Mouse IgG antibody (STAR13B) used to detect bound mouse IgG antibodies in an ELISA designed to assess FhCL1 specific antibody responses in sheep.
Image caption:
Levels of IgG1 (a) and IgG2 (b) that recognise FhCL1. Each line represents the mean values obtained at each time point, bars indicate standard error, asterisks indicate statistical significance (P <0.001)
From: Buffoni L, Garza-Cuartero L, Pérez-Caballero R, Zafra R, Javier Martínez-Moreno F, Molina-Hernández V, Pérez J, Martínez-Moreno Á, Mulcahy G.
Identification of protective peptides of Fasciola hepatica-derived cathepsin L1 (FhCL1) in vaccinated sheep by a linear B-cell epitope mapping approach.
Parasit Vectors. 2020 Jul 31;13(1):390.
doi: 10.1186/s13071-020-04260-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Rabbit anti Mouse IgG antibody (STAR13B) used to detect bound Mouse anti HIS tag antibody in a western blotting assay.
Image caption:
Western blot analysis of HspX-PPE44-EsxV transfected Chinese hamster ovary (CHO) cells (lane 1) and untransfected CHO cells (lane 2) and lane m: protein size marker (Fermentas Company, Germany)
From: Moradi B, Sankian M, Amini Y, Gholoobi A, Meshkat Z.
A new DNA vaccine expressing HspX-PPE44-EsxV fusion antigens of Mycobacterium tuberculosis. induced strong immune responses.
Iran J Basic Med Sci. 2020 Jul;23(7):909-14.
doi: 10.22038/ijbms.2020.38521.9171.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Mouse IgG antibody recognizes all subclasses of murine IgG. Some cross reactivity with IgM will be expected.
Cross reactivity is expected with rat IgG. Cross reactivity with human serum has been minimised by solid phase adsorbtion.
Cross reactivity is expected with rat IgG. Cross reactivity with human serum has been minimised by solid phase adsorbtion.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Preparation
- Purified IgG framents were prepared by affinity chromatography of serum.
F(ab)2 fragments were prepared by pepsin digestion, followed by gel filtration to remove any intact IgG or Fc fragments. - Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Purified whole mouse immunoglobulin G.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01869
- P01865
- P01864
- P01868
- P03987
- P01867
- P01863
- Entrez Gene
- Igh-1a
- Igh-1a
- Igh-1a
- Ighg2b
- Ighg1
- Ighg1
- AI324046
- GO Terms
- GO:0003823 antigen binding
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0050766 positive regulation of phagocytosis
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | 1/1000 | |
| Immunohistology - Frozen | 1/50 | ||
| Immunohistology - Paraffin | 1/50 | ||
| Immunohistology - Resin | 1/50 | ||
| Western Blotting | 1/2000 | 1/5000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG antibody
-
Buffoni, L. et al. (2012) Humoral immune response in goats immunised with cathepsin L1, peroxiredoxin and Sm14 antigen and experimentally challenged with Fasciola hepatica.
Vet Parasitol. 185: 315-21. -
Emara, M. et al. (2012) Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite.
J Biol Chem. 287: 5756-63. -
Johnson, A.E. et al. (2009) AZD2184: a radioligand for sensitive detection of beta-amyloid deposits.
J Neurochem. 108: 1177-86. -
Raida, M.K. et al. (2011) Association between plasma antibody response and protection in rainbow trout Oncorhynchus mykiss immersion vaccinated against Yersinia ruckeri.
PLoS One. 6: e18832. -
Scott, J.L. et al. (2006) Leucocyte population changes in the reproductive tract of the ewe in response to insemination.
Reprod Fertil Dev. 18: 627-34. -
Scott, J.L. et al. (2007) Granulocyte-macrophage colony stimulating factor and interleukin-8 in the reproductive tract of ewes following oestrus and mating.
Reprod Fertil Dev. 19: 585-93. -
Scott, J.L. et al. (2009) Spermatozoa and seminal plasma induce a greater inflammatory response in the ovine uterus at oestrus than dioestrus.
Reprod Fertil Dev. 21: 817-26. -
Siepe, M. et al. (2006) Myoblast-seeded biodegradable scaffolds to prevent post-myocardial infarction evolution toward heart failure.
J Thorac Cardiovasc Surg. 132: 124-31. -
Siepe, M. et al. (2007) Construction of skeletal myoblast-based polyurethane scaffolds for myocardial repair.
Artif Organs. 31: 425-33. -
von Gersdorff Jφrgensen, L. et al. (2011) Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifilii (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum).
J Fish Dis. 34: 749-55. -
Yata, Y. et al. (1999) An improved method for the purification of stellate cells from rat liver with dichloromethylene diphosphate (CL2MDP).
Methods Cell Sci. 21: 19-24. -
Skov, J. et al. (2012) Immunomodulatory effects of dietary β-1,3-glucan from Euglena gracilis in rainbow trout (Oncorhynchus mykiss) immersion vaccinated against Yersinia ruckeri.
Fish Shellfish Immunol. 33: 111-20. -
Chettri, J.K. et al. (2013) Comparative evaluation of administration methods for a vaccine protecting rainbow trout against Yersinia ruckeri O1 biotype 2 infections.
Vet Immunol Immunopathol. 154: 42-7. -
Villumsen, K.R. et al. (2014) Oral and Anal Vaccination Confers Full Protection against Enteric Redmouth Disease (ERM) in Rainbow Trout.
PLoS One. 9(4):e93845. -
Moradi, B. et al. (2016) Construction of a Novel DNA Vaccine Candidate Encoding an HspX-PPE44-EsxV Fusion Antigen of Mycobacterium tuberculosis.
Rep Biochem Mol Biol. 4 (2): 89-97. -
Marana, M.H. et al. (2017) Positive correlation between Aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout Oncorhynchus mykiss evaluated by a tail fin infection model.
J Fish Dis. 40 (4): 507-16. -
Jaafar RM et al. (2015) Effects of adjuvant Montanide™ ISA 763 A VG in rainbow trout injection vaccinated against Yersinia ruckeri.
Fish Shellfish Immunol. 47 (2): 797-806. -
Buffoni, L. et al. (2020) Identification of protective peptides of Fasciola hepatica-derived cathepsin L1 (FhCL1) in vaccinated sheep by a linear B-cell epitope mapping approach.
Parasit Vectors. 13 (1): 390. -
Moradi, B. et al. (2020) A new DNA vaccine expressing HspX-PPE44-EsxV fusion antigens of Mycobacterium tuberculosis induced strong immune responses.
Iran J Basic Med Sci. 23 (7): 909-14. -
Zafra, R. et al. (2021) Efficacy of a multivalent vaccine against Fasciola hepatica. infection in sheep.
Vet Res. 52 (1): 13. -
Pérez-Caballero, R. et al. (2018) Expression of free radicals by peritoneal cells of sheep during the early stages of Fasciola hepatica. infection.
Parasit Vectors. 11 (1): 500.
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