IgG antibody

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Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
STAR13B
Datasheet
C E P RE WB
MSDS
1 mg loader

Rabbit anti Mouse IgG antibody recognizes all subclasses of murine IgG. Some cross reactivity with IgM will be expected.

Cross reactivity is expected with rat IgG. Cross reactivity with human serum has been minimised by solid phase adsorbtion.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG framents were prepared by affinity chromatography of serum.

F(ab)2 fragments were prepared by pepsin digestion, followed by gel filtration to remove any intact IgG or Fc fragments.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Purified whole mouse immunoglobulin G.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01869
P01865
P01864
P01868
P03987
P01867
P01863
Entrez Gene
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/1000
Immunohistology - Frozen 1/50
Immunohistology - Paraffin 1/50
Immunohistology - Resin 1/50
Western Blotting 1/2000 1/5000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Application Based External Images

ELISA

Western Blotting

Product Specific References

References for IgG antibody

  1. Buffoni, L. et al. (2012) Humoral immune response in goats immunised with cathepsin L1, peroxiredoxin and Sm14 antigen and experimentally challenged with Fasciola hepatica.
    Vet Parasitol. 185: 315-21.
  2. Emara, M. et al. (2012) Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite.
    J Biol Chem. 287: 5756-63.
  3. Johnson, A.E. et al. (2009) AZD2184: a radioligand for sensitive detection of beta-amyloid deposits.
    J Neurochem. 108: 1177-86.
  4. Raida, M.K. et al. (2011) Association between plasma antibody response and protection in rainbow trout Oncorhynchus mykiss immersion vaccinated against Yersinia ruckeri.
    PLoS One. 6: e18832.
  5. Scott, J.L. et al. (2006) Leucocyte population changes in the reproductive tract of the ewe in response to insemination.
    Reprod Fertil Dev. 18: 627-34.
  6. Scott, J.L. et al. (2007) Granulocyte-macrophage colony stimulating factor and interleukin-8 in the reproductive tract of ewes following oestrus and mating.
    Reprod Fertil Dev. 19: 585-93.
  7. Scott, J.L. et al. (2009) Spermatozoa and seminal plasma induce a greater inflammatory response in the ovine uterus at oestrus than dioestrus.
    Reprod Fertil Dev. 21: 817-26.
  8. Siepe, M. et al. (2006) Myoblast-seeded biodegradable scaffolds to prevent post-myocardial infarction evolution toward heart failure.
    J Thorac Cardiovasc Surg. 132: 124-31.
  9. Siepe, M. et al. (2007) Construction of skeletal myoblast-based polyurethane scaffolds for myocardial repair.
    Artif Organs. 31: 425-33.
  10. von Gersdorff Jφrgensen, L. et al. (2011) Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifilii (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum).
    J Fish Dis. 34: 749-55.
  11. Yata, Y. et al. (1999) An improved method for the purification of stellate cells from rat liver with dichloromethylene diphosphate (CL2MDP).
    Methods Cell Sci. 21: 19-24.
  12. Skov, J. et al. (2012) Immunomodulatory effects of dietary β-1,3-glucan from Euglena gracilis in rainbow trout (Oncorhynchus mykiss) immersion vaccinated against Yersinia ruckeri.
    Fish Shellfish Immunol. 33: 111-20.
  13. Chettri, J.K. et al. (2013) Comparative evaluation of administration methods for a vaccine protecting rainbow trout against Yersinia ruckeri O1 biotype 2 infections.
    Vet Immunol Immunopathol. 154: 42-7.
  14. Villumsen, K.R. et al. (2014) Oral and Anal Vaccination Confers Full Protection against Enteric Redmouth Disease (ERM) in Rainbow Trout.
    PLoS One. 9(4):e93845.
  15. Moradi, B. et al. (2016) Construction of a Novel DNA Vaccine Candidate Encoding an HspX-PPE44-EsxV Fusion Antigen of Mycobacterium tuberculosis.
    Rep Biochem Mol Biol. 4 (2): 89-97.
  16. Marana, M.H. et al. (2017) Positive correlation between Aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout Oncorhynchus mykiss evaluated by a tail fin infection model.
    J Fish Dis. 40 (4): 507-16.
  17. Jaafar RM et al. (2015) Effects of adjuvant Montanide™ ISA 763 A VG in rainbow trout injection vaccinated against Yersinia ruckeri.
    Fish Shellfish Immunol. 47 (2): 797-806.