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IgG antibody

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Rabbit F(ab')2 anti Mouse IgG:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

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Biotin conjugated Rabbit F(ab')2 anti Mouse IgG antibody recognizes all subclasses of mouse IgG.

Cross reactivity with rat IgG is expected. Cross reactivity to human serum proteins has been minimised by solid phase adsorbtion.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
F(ab')2 fragment of IgG conjugated to biotin - liquid
Antiserum Preparation
Antisera to Mouse IgG were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified mouse IgG.
Approx. Protein Concentrations
F(ab')2 concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/2000
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1/50 1/200
Immunohistology - Paraffin 1/50 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

References for IgG antibody

  1. Silveira, M.R. et al. (2002) Infection with Strongyloides venezuelensis induces transient airway eosinophilic inflammation, an increase in immunoglobulin E, and hyperresponsiveness in rats.
    Infect Immun. 70 (11): 6263-72.
  2. Conlon, T.M. et al. (2012) Germinal center alloantibody responses are mediated exclusively by indirect-pathway CD4 T follicular helper cells.
    J Immunol. 188 (6): 2643-52.
  3. Harper, I.G. et al. (2016) Augmentation of Recipient Adaptive Alloimmunity by Donor Passenger Lymphocytes within the Transplant.
    Cell Rep. 15 (6): 1214-27.

Further Reading

  1. Motallebzadeh, R. et al. (2012) Blocking lymphotoxin signaling abrogates the development of ectopic lymphoid tissue within cardiac allografts and inhibits effector antibody responses.
    FASEB J. 26 (1): 51-62.

RRID
AB_321919
UniProt
P01869
P03987
P01867
P01868
P01865
P01864
P01863
Entrez Gene
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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