IgG antibody

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Rabbit F(ab')2 anti Mouse IgG:Alk. Phos.

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: IgG

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Rabbit anti Mouse IgG antibody recognises mouse IgG.

Cross reactivity is observed with rat IgG. Cross-reactivity with human IgG has been minimised by solid phase absorption.

Target Species: Mouse
Species Cross-Reactivity: Target Species Cross Reactivity

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: F(ab')₂ fragment of rabbit IgG conjugated to Alkaline Phosphatase - liquid
Antiserum Preparation: Antisera to mouse IgG were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
F(ab')₂ fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.
Buffer Solution: 0.05M TRIS Chloride
0.15M NaCl
0.001M MgCl₂
0.0001M ZnCl₂
50% (v/v) Glycerol; pH8.0
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Immunogen: Mouse IgG.
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Rat

Store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/1000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

RRID: AB_323493
UniProt: P01869; P01865; P03987; P01867; P01864; P01868; P01863
Entrez Gene: Igh-1a; Igh-1a; Igh-1a; Ighg2b; Ighg1; Ighg1; AI324046
GO Terms: GO:0003823 antigen binding; GO:0002455 humoral immune response mediated by circulating immunoglobulin; GO:0019731 antibacterial humoral response; GO:0042571 immunoglobulin complex, circulating; GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0005576 extracellular region; GO:0001788 antibody-dependent cellular cytotoxicity; GO:0001798 positive regulation of type IIa hypersensitivity; View More GO Terms; GO:0001812 positive regulation of type I hypersensitivity; GO:0006910 phagocytosis, recognition; GO:0006911 phagocytosis, engulfment; GO:0006958 complement activation, classical pathway; GO:0050766 positive regulation of phagocytosis