IgG antibody

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Anti Mouse IgG (H/L) Antibody gallery image 1 — **Published customer image:**
FITC conjugated Goat anti Mouse IgG (H/L) antibody (**5178-2404**) used as a secondary reagent for the detection and evaluation of bound mouse primary antibodies on human adipose derived stem cells by flow cytometry.
**Image caption:**
Analysis of hASC markers.
(A) CD90. (B) CD105. (C) Nanog. (D) Sox-2. (E) STRO-1. (F) CD45RO. (G) CD117. (H) Quantification of expression of positive and negative markers. Graphs show the expression of different markers in hASCs. The expression of the markers was assessed using flow cytometry by means of the intensity of the emitted fluorescence (FL1 and FL2). The expression of the positive markers, CD90, CD105, and STRO-1 (surface markers mesenchymal) and Nanog and Sox-2 (pluripotency markers) is compared to the decreased expression of negative markers (CD45RO and CD117). Data are represented as mean ± standard deviation. The statistical analyses were obtained using an ANOVA followed by Dunnett's test with P <0.05 being considered significant.

From: Olimpio RMC, de Oliveira M, De Sibio MT, Moretto FCF, Deprá IC, Mathias LS, *et al*. (2018)
Cell viability assessed in a reproducible model of human osteoblasts derived from human adipose-derived stem cells.
PLoS ONE 13(4): e0194847.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Goat anti Mouse IgG (H/L):FITC

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
5178-2404	F IF			2 mg

Summary
References (2)

Goat anti Mouse IgG antibody recognizes the heavy and light chains of mouse IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, mouse IgG and mouse serum.

Product Details

Target Species: Mouse
Product Form: Purified Ig conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilised
Reconstitution: Use 1.0 ml of DI water. For extended storage, reconstitute with 50% glycerol instead of water and then aliquot contents at -20ºC.
Preparation: Purified Ig prepared by affinity chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 10 mg/ml Bovine Serum Albumin and 0.01% sodium azide
Immunogen: Native, whole IgG
Approx. Protein Concentrations: IgG concentration 2.0 mg/ml

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: P01868 Related reagents; P01869 Related reagents; P01843 Related reagents; P01845 Related reagents; P01867 Related reagents; P01865 Related reagents; P01863 Related reagents; P01864 Related reagents; P03987 Related reagents; P01844 Related reagents; P01837 Related reagents
Entrez Gene: Igk-C Related reagents; LOC433053 Related reagents; Iglc2 Related reagents; Iglc3 Related reagents; Igh-1a Related reagents; Igh-1a Related reagents; Igh-1a Related reagents; Ighg2b Related reagents; Ighg1 Related reagents; Ighg1 Related reagents; AI324046 Related reagents
GO Terms: GO:0003823 antigen binding; GO:0009897 external side of plasma membrane; GO:0019815 B cell receptor complex; GO:0002455 humoral immune response mediated by circulating immunoglobulin; GO:0019731 antibacterial humoral response; GO:0042571 immunoglobulin complex, circulating; GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0005576 extracellular region; GO:0001788 antibody-dependent cellular cytotoxicity; GO:0001798 positive regulation of type IIa hypersensitivity; GO:0001812 positive regulation of type I hypersensitivity; GO:0006910 phagocytosis, recognition; GO:0006911 phagocytosis, engulfment; GO:0006958 complement activation, classical pathway; GO:0050766 positive regulation of phagocytosis
Regulatory: For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		1/2K	1/10K
Immunofluorescence		1/500	1/2500

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

References for IgG antibody

Olimpio, R.M.C. et al. (2018) Cell viability assessed in a reproducible model of human osteoblasts derived from human adipose-derived stem cells.
PLoS One. 13 (4): e0194847.