IgG antibody
FITC conjugated Goat anti Mouse IgG (H/L) antibody (5178-2404) used as a secondary reagent for the detection and evaluation of bound mouse primary antibodies on human adipose derived stem cells by flow cytometry.
Image caption:
Analysis of hASC markers.
(A) CD90. (B) CD105. (C) Nanog. (D) Sox-2. (E) STRO-1. (F) CD45RO. (G) CD117. (H) Quantification of expression of positive and negative markers. Graphs show the expression of different markers in hASCs. The expression of the markers was assessed using flow cytometry by means of the intensity of the emitted fluorescence (FL1 and FL2). The expression of the positive markers, CD90, CD105, and STRO-1 (surface markers mesenchymal) and Nanog and Sox-2 (pluripotency markers) is compared to the decreased expression of negative markers (CD45RO and CD117). Data are represented as mean ± standard deviation. The statistical analyses were obtained using an ANOVA followed by Dunnett's test with P <0.05 being considered significant.
From: Olimpio RMC, de Oliveira M, De Sibio MT, Moretto FCF, Deprá IC, Mathias LS, et al. (2018)
Cell viability assessed in a reproducible model of human osteoblasts derived from human adipose-derived stem cells.
PLoS ONE 13(4): e0194847.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
F ResetGoat anti Mouse IgG (H/L):FITC
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
- Region
- (H/L)
Antibody Quality and Performance Guarantee
| Goat anti Mouse IgG antibody recognizes the heavy and light chains of mouse IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, mouse IgG and mouse serum. |
- Target Species
- Mouse
- Product Form
- Purified Ig conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilized
- Reconstitution
- Use 1.0 ml of DI water. For extended storage, reconstitute with 50% glycerol instead of water and then aliquot contents at -20ºC.
- Preparation
- Purified Ig prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10 mg/ml Bovine Serum Albumin and 0.01% sodium azide
- Immunogen
- Native, whole IgG
- Approx. Protein Concentrations
- IgG concentration 2.0 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/2K | 1/10K |
| Immunofluorescence | |
1/500 | 1/2500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
References for IgG antibody
-
Olimpio, R.M.C. et al. (2018) Cell viability assessed in a reproducible model of human osteoblasts derived from human adipose-derived stem cells.
PLoS One. 13 (4): e0194847.
Further Reading
-
The, T.H. & Feltkamp, T.E. (1970) Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.
Immunology. 18 (6): 865-73.
- RRID
- AB_616780
- UniProt
- P01868
- P01869
- P01843
- P01845
- P01867
- P01865
- P01863
- P01864
- P03987
- P01844
- P01837
- Entrez Gene
- Igk-C
- LOC433053
- Iglc2
- Iglc3
- Igh-1a
- Igh-1a
- Igh-1a
- Ighg2b
- Ighg1
- Ighg1
- AI324046
- GO Terms
- GO:0003823 antigen binding
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- View More GO Terms
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0050766 positive regulation of phagocytosis
5178-2404
153989
170410
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