IgG antibody

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Goat ANTI-MOUSE IgG StarBright™ Blue 520

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
12005867
Datasheet
WB
MSDS
80 µl loader

12005866
Datasheet
WB
MSDS
400 µl loader

The StarBright Blue 520 Fluorophore is an ultra-sensitive polymer-based fluorophore with an excitation maximum at 488 nm and emission maximum at 520 nm. The StarBright Fluorophore is made up of multiple light-absorbing and -emitting monomers which provide an exceptionally bright signal compared to most traditional fluorophores.

StarBright Blue 520 Conjugated Goat Anti-Mouse and Goat Anti-Rabbit Fluorescent Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single-target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. StarBright Blue 520 Fluorescent Secondary Antibodies can be used with far-red emission StarBright Blue 700 Antibodies as well as traditional fluorescent detection reagents like Alexa Fluor and DyLight Conjugated Antibodies. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or with hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot.

Features and Benefits

Easy multiplexing — can be used with traditional fluorescent antibodies or with Bio-Rad's StarBright Blue 700 Fluorescent Secondary Antibodies to detect multiple proteins on the same blot
High sensitivity — StarBright Blue 520 Fluorescent Secondary Antibodies (Exmax/Emmax = 488 nm/520 nm) have 2–4 fold higher sensitivity compared to traditional green emitters like Alexa Fluor 488 or DyLight 488 Antibodies
Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes
Low nonspecific binding — conjugated to highly cross-adsorbed antibodies

Goat Anti-Mouse IgG StarBright Blue 520 has been cross-adsorbed against bovine, goat, human, rabbit and rat.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to StarBright Blue 520 - lyophilized
Reconstitution
Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at max speed in a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube. Do not centrifuge more than 10 sec.
Antiserum Preparation
Antiserum to Mouse IgG was raised by repeated immunisation of Goats with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
>80% Sucrose
<1% Polyethylene Glycol
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% Bovine Serum Albumin

Storage Information

Storage
1 year at -20oC lyophilized; 6 months in the dark at +4oC after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/2,500 1/5,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
StarBright Blue 520 Fluorescent Secondary Antibodies can be used for multiplex fluorescent western blotting with Bio-Rad's ultra-sensitive near IR StarBright Blue 700 Antibodies and the hFAB Rhodamine Anti-Housekeeping Antibodies.
Note: StarBright Antibodies are not suitable for stripping and reprobing.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12005866.pdf