IgG antibody
Texas red conjugated Goat anti Mouse IgG antibody (103007) used for the detection of bound anti CD80 antibody by immunofluorescence
Image caption:
Immunolabelling of Mφ by the monocyte/Mφ marker CD14 and the M1 Mφ marker CD80. Mφ were seeded at a density of 5.7×104 cells/cm2, with (coculture) or without the same number of MDA-MB-231 cells. In each case, the culture medium was replaced daily. 5 days later, all cultures were treated with 1 μg/ml LPS and 5 ng/ml IFNγ for 24 h before washing and labelling. CD14 fluorescent labelling is green, CD80 is red. Panel A: without TM, it can be seen that CD80 was expressed on activated Mφ cultured alone and was not detectable on Mφ in the cocultures. Panel B: in presence of TM, no CD80-positive cells were found in any of the cultures.
From: Joimel U, Gest C, Soria J, Pritchard LL, Alexandre J, Laurent M, Blot E, Cazin L, Vannier JP, Varin R, Li H, Soria C.
Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.
BMC Cancer. 2010 Jul 17;10:375.
Biotin conjugated Goat anti Mouse IgG (103008) used in conjunction with horseradish peroxidase conjugated streptavidin (710005) for the detection of anti tumor derived antigen IgG by ELISA.
Image caption:
Local melanoma tumors generate systemic circulating IgG levels. (A). Outline of local tumor injection experiment. B16.F10-tdTom+ cells were s.c. injected into C57BL/6 mice on day 0 and allowed to proliferate for 30 days. In some mice, B16.F10-tdTom lysate was additionally s.c. injected to test whether IgG production can be boosted. (B) Brightfield (top) and red fluorescent (bottom) stereomicroscopic images of tumor-injected and contralateral footpads on day 30. (C) IgG titers in control and tumor-bearing mice on day 30, with and without additional B16.F10-tdTom+ cell lysate injection for 15 days. Each dot represents the serum of one mouse. Data in (C) are pooled from two independent experiments and were analyzed by a Kruskal–Wallis test with Dunn’s multiple comparison test. **p <0.01, ***p <0.001.
From: F. Moalli, S. T. Proulx, R. Schwendener, M. Detmar, C. Schlapbach, and J. V. Stein, “Intravital and Whole-Organ Imaging Reveals Capture of Melanoma-Derived Antigen by Lymph Node Subcapsular Macrophages Leading to Widespread Deposition on Follicular Dendritic Cells,”
Front. Immunol., vol. 6, Mar. 2015.
Texas Red® conjugated Goat anti Mouse IgG antibody (103007) used for the detection of bound Mouse anti PSGL-1 antibody on neutrophil differentiated HL-60 cells by immunofluorescence.
Image caption:
Recognition of S. pneumoniae by PSGL-1 is a generic event. Co-localization of FAMS-SE-labeled S. pneumoniae clinical isolates of different serotypes with PSGL-1 in HL-60 cells differentiated to neutrophils. Cellular DNA was stained with Hoechst whereas PSGL-1 was visualized with mouse anti-human PSGL-1 antibody followed by a goat anti-mouse Texas-Red staining. (DIC, Differential interference contrast).
From: Ramos-Sevillano E, Urzainqui A, de Andrés B, González-Tajuelo R, Domenech M, González-Camacho F, et al. (2016)
PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.
PLoS Pathog 12(3): e1005500.
Texas Red® conjugated Goat anti Mouse IgG antibody (103007) used for the detection of bound Mouse anti PSGL-1 antibody on neutrophil differentiated HL-60 cells by immunofluorescence.
Image caption:
Local co-localization of FAM-SE S. pneumoniae (green), Cellular DNA (blue) and PSGL-1 (red) is shown and quantified by plotting the fluorescence intensity and the distance (in μm).
From: Ramos-Sevillano E, Urzainqui A, de Andrés B, González-Tajuelo R, Domenech M, González-Camacho F, et al. (2016)
PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.
PLoS Pathog 12(3): e1005500.
Goat anti Mouse IgG:Alk. Phos.
Goat anti Mouse IgG:Biotin
Goat anti Mouse IgG:FITC
Goat anti Mouse IgG:HRP
Goat anti Mouse IgG
Goat anti Mouse IgG:RPE
Goat anti Mouse IgG:TRITC
Goat anti Mouse IgG:Texas Red®
Goat anti Mouse IgG:BGAL
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| 103004 | E | 1 ml |
|
|
| 103008 | E IB IF | 1 mg |
|
|
| 103001F | F IF | 1 mg |
|
|
| 103005 | E | 1 ml |
|
|
| 103001 | E IB | 1 mg |
|
|
| 103009 | F IF | 0.5 mg |
|
|
| 103003 | IF | 1 mg |
|
|
| 103007 | IF | 1 mg |
|
|
| 103006 | E | 1 ml |
|
Goat anti Mouse IgG antibody recognizes mouse IgG, recognising the heavy chain of mouse IgG1, IgG2a, IgG2b and IgG3 as demonstrated by ELISA.
Goat anti Mouse IgG antibody has been cross-adsorbed against mouse IgM, mouse IgA and human serum to reduce potential cross-reactivity.
Goat anti Mouse IgG antibody has been cross-adsorbed against mouse IgM, mouse IgA and human serum to reduce potential cross-reactivity.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Ig fraction - liquid
- Product Form
- Purified Ig conjugated to R. Phycoerythrin (RPE) - liquid
- Product Form
- Ig Fraction conjugated to Rhodamine (TRITC) - liquid
- Product Form
- Purified IgG conjugated to Texas Red® - liquid
- Product Form
- Purfied IgG conjugated to Beta Galactosidase - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
- Preparation
- Purified IgG prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose.
- Preparation
- Purified Ig prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
- Preparation
- Purified Ig prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
- Preparation
- Purified Ig prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
- Preparation
- Purified Ig prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
- Antiserum Preparation
- Antisera to mouse IgG were raised by repeated immunisations of goats with highly purified antigen.
- Buffer Solution
- TRIS buffered saline, 1mM MgCl2
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Borate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide 50% Glycerol - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
50% Glycerol - Preservative Stabilisers
- None Present
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
Stabilizing agent - Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) 50% Glycerol - Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- Ig concentration 1.0 mg/ml
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Approx. Protein Concentrations
- Ig concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P01867
- P01865
- P01863
- P01864
- P01868
- P01869
- P03987
- Entrez Gene
- Igh-1a
- Igh-1a
- Igh-1a
- Ighg2b
- Ighg1
- Ighg1
- AI324046
- GO Terms
- GO:0003823 antigen binding
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0050766 positive regulation of phagocytosis
- Acknowledgements
- Texas Red® (TXRD) is a registered trademark on Molecular Probes, Inc.
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | 1/4000 | |
| Flow Cytometry | |||
| ELISA | 1/5,000 | 1/20,000 | |
| Immunoblotting | |||
| Immunofluorescence | |||
| Flow Cytometry | |||
| Immunofluorescence | |||
| ELISA | 1/2000 | 1/5000 | |
| ELISA | 1/2000 | 1/4000 | |
| Immunoblotting | |||
| Flow Cytometry | |||
| Immunofluorescence | |||
| Immunofluorescence | |||
| Immunofluorescence | |||
| ELISA | 1/200 | 1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG antibody
-
Joimel, U. et al. (2010) Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.
BMC Cancer. 10: 375. -
Childs K et al. (2012) Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction.
J Virol. 86 (7): 3411-21. -
Moalli, F. et al. (2015) Intravital and whole-organ imaging reveals capture of melanoma-derived antigen by lymph node subcapsular macrophages leading to widespread deposition on follicular dendritic cells.
Front Immunol. 6: 114. -
Ramos-Sevillano, E. et al. (2016) PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.
PLoS Pathog. 12 (3): e1005500. -
Abbate, F. et al. (2016) Acid-sensing ion channel immunoreactivities in the cephalic neuromasts of adult zebrafish.
Ann Anat. 207: 27-31.
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