IgG antibody
Texas red conjugated Goat anti Mouse IgG antibody (103007) used for the detection of bound anti CD80 antibody by immunofluorescence
Image caption:
Immunolabelling of Mφ by the monocyte/Mφ marker CD14 and the M1 Mφ marker CD80. Mφ were seeded at a density of 5.7×104 cells/cm2, with (coculture) or without the same number of MDA-MB-231 cells. In each case, the culture medium was replaced daily. 5 days later, all cultures were treated with 1 μg/ml LPS and 5 ng/ml IFNγ for 24 h before washing and labelling. CD14 fluorescent labelling is green, CD80 is red. Panel A: without TM, it can be seen that CD80 was expressed on activated Mφ cultured alone and was not detectable on Mφ in the cocultures. Panel B: in presence of TM, no CD80-positive cells were found in any of the cultures.
From: Joimel U, Gest C, Soria J, Pritchard LL, Alexandre J, Laurent M, Blot E, Cazin L, Vannier JP, Varin R, Li H, Soria C.
Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.
BMC Cancer. 2010 Jul 17;10:375.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Biotin conjugated Goat anti Mouse IgG (103008) used in conjunction with horseradish peroxidase conjugated streptavidin for the detection of anti tumor derived antigen IgG by ELISA.
Image caption:
Local melanoma tumors generate systemic circulating IgG levels. (A). Outline of local tumor injection experiment. B16.F10-tdTom+ cells were s.c. injected into C57BL/6 mice on day 0 and allowed to proliferate for 30 days. In some mice, B16.F10-tdTom lysate was additionally s.c. injected to test whether IgG production can be boosted. (B) Brightfield (top) and red fluorescent (bottom) stereomicroscopic images of tumor-injected and contralateral footpads on day 30. (C) IgG titers in control and tumor-bearing mice on day 30, with and without additional B16.F10-tdTom+ cell lysate injection for 15 days. Each dot represents the serum of one mouse. Data in (C) are pooled from two independent experiments and were analyzed by a Kruskal–Wallis test with Dunn’s multiple comparison test. **p <0.01, ***p <0.001.
From: F. Moalli, S. T. Proulx, R. Schwendener, M. Detmar, C. Schlapbach, and J. V. Stein, “Intravital and Whole-Organ Imaging Reveals Capture of Melanoma-Derived Antigen by Lymph Node Subcapsular Macrophages Leading to Widespread Deposition on Follicular Dendritic Cells,”
Front. Immunol., vol. 6, Mar. 2015.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Texas Red® conjugated Goat anti Mouse IgG antibody (103007) used for the detection of bound Mouse anti PSGL-1 antibody on neutrophil differentiated HL-60 cells by immunofluorescence.
Image caption:
Recognition of S. pneumoniae by PSGL-1 is a generic event. Co-localization of FAMS-SE-labeled S. pneumoniae clinical isolates of different serotypes with PSGL-1 in HL-60 cells differentiated to neutrophils. Cellular DNA was stained with Hoechst whereas PSGL-1 was visualized with mouse anti-human PSGL-1 antibody followed by a goat anti-mouse Texas-Red staining. (DIC, Differential interference contrast).
From: Ramos-Sevillano E, Urzainqui A, de Andrés B, González-Tajuelo R, Domenech M, González-Camacho F, et al. (2016)
PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.
PLoS Pathog 12(3): e1005500.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Texas Red® conjugated Goat anti Mouse IgG antibody (103007) used for the detection of bound Mouse anti PSGL-1 antibody on neutrophil differentiated HL-60 cells by immunofluorescence.
Image caption:
Local co-localization of FAM-SE S. pneumoniae (green), Cellular DNA (blue) and PSGL-1 (red) is shown and quantified by plotting the fluorescence intensity and the distance (in μm).
From: Ramos-Sevillano E, Urzainqui A, de Andrés B, González-Tajuelo R, Domenech M, González-Camacho F, et al. (2016)
PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.
PLoS Pathog 12(3): e1005500.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Fitc and RPE conjugated Goat anti Mouse IgG antibody (103001F and 103009) used to label bound mouse antibodies against canine determinants for immunofluorescence.
Image caption:
Structural organization of canine Peyer’s patches, according to immunofluorescence data.
(C) Immunofluorescence staining of PPs. PPs were stained with DAPI in combination with Abs against CD8 and one of the following markers: CD21, CD4, or FOXP3. Epifluorescence microscopy is shown at original magnification ×10, visualizing specific structures, including the subepithelial dome (SED), T cell areas, and B cell follicles.
From: Miguelena Chamorro B, Hameed SA, Dechelette M, Claude JB, Piney L, Chapat L, Swaminathan G, Poulet H, Longet S, De Luca K, Mundt E, Paul S.
Characterization of Canine Peyer's Patches by Multidimensional Analysis: Insights from Immunofluorescence, Flow Cytometry, and Single-Cell RNA Sequencing.
Immunohorizons. 2023 Nov 1;7(11):788-805.
doi: 10.4049/immunohorizons.2300091.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Fitc and RPE conjugated Goat anti Mouse IgG antibody (103001F and 103009) used to label bound mouse antibodies against canine determinants for immunofluorescence.
Image caption:
Structural organization of canine Peyer’s patches, according to immunofluorescence data.
(C) Immunofluorescence staining of PPs. PPs were stained with DAPI in combination with Abs against CD8 and one of the following markers: CD21, CD4, or FOXP3. Epifluorescence microscopy is shown at original magnification ×10, visualizing specific structures, including the subepithelial dome (SED), T cell areas, and B cell follicles.
From: Miguelena Chamorro B, Hameed SA, Dechelette M, Claude JB, Piney L, Chapat L, Swaminathan G, Poulet H, Longet S, De Luca K, Mundt E, Paul S.
Characterization of Canine Peyer's Patches by Multidimensional Analysis: Insights from Immunofluorescence, Flow Cytometry, and Single-Cell RNA Sequencing.
Immunohorizons. 2023 Nov 1;7(11):788-805.
doi: 10.4049/immunohorizons.2300091.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Fitc and RPE conjugated Goat anti Mouse IgG antibody (103001F and 103009) used to label bound mouse antibodies against canine determinants for immunofluorescence.
Image caption:
Structural organization of canine Peyer’s patches, according to immunofluorescence data. (D) Myeloid cell staining. Immunostaining of the CD14 and CD11c markers, along with CD21, was used to identify dendritic cells within the SED. Different images from the lower and upper parts of the sample were captured to adequately show the CD11c staining in proximity to both the epithelium and the follicle.
From: Miguelena Chamorro B, Hameed SA, Dechelette M, Claude JB, Piney L, Chapat L, Swaminathan G, Poulet H, Longet S, De Luca K, Mundt E, Paul S.
Characterization of Canine Peyer's Patches by Multidimensional Analysis: Insights from Immunofluorescence, Flow Cytometry, and Single-Cell RNA Sequencing.
Immunohorizons. 2023 Nov 1;7(11):788-805.
doi: 10.4049/immunohorizons.2300091.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Fitc and RPE conjugated Goat anti Mouse IgG antibody (103001F and 103009) used to label bound mouse antibodies against canine determinants for immunofluorescence.
Image caption:
Structural organization of canine Peyer’s patches, according to immunofluorescence data. (D) Myeloid cell staining. Immunostaining of the CD14 and CD11c markers, along with CD21, was used to identify dendritic cells within the SED. Different images from the lower and upper parts of the sample were captured to adequately show the CD11c staining in proximity to both the epithelium and the follicle.
From: Miguelena Chamorro B, Hameed SA, Dechelette M, Claude JB, Piney L, Chapat L, Swaminathan G, Poulet H, Longet S, De Luca K, Mundt E, Paul S.
Characterization of Canine Peyer's Patches by Multidimensional Analysis: Insights from Immunofluorescence, Flow Cytometry, and Single-Cell RNA Sequencing.
Immunohorizons. 2023 Nov 1;7(11):788-805.
doi: 10.4049/immunohorizons.2300091.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Mouse IgG
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
Antibody Quality and Performance Guarantee
| Goat anti Mouse IgG antibody recognizes mouse IgG, recognising the heavy chain of mouse IgG1, IgG2a, IgG2b and IgG3 as demonstrated by ELISA. Goat anti Mouse IgG antibody has been cross-adsorbed against mouse IgM, mouse IgA and human serum to reduce potential cross-reactivity. |
- Target Species
- Mouse
- Product Form
- Ig fraction - liquid
- Preparation
- Purified Ig prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
- Antiserum Preparation
- Antisera to mouse IgG were raised by repeated immunisations of goats with highly purified antigen.
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- None Present
- Approx. Protein Concentrations
- Ig concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/2000 | 1/4000 |
| Immunoblotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
References for IgG antibody
-
Joimel, U. et al. (2010) Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.
BMC Cancer. 10: 375. -
Childs K et al. (2012) Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction.
J Virol. 86 (7): 3411-21. -
Moalli, F. et al. (2015) Intravital and whole-organ imaging reveals capture of melanoma-derived antigen by lymph node subcapsular macrophages leading to widespread deposition on follicular dendritic cells.
Front Immunol. 6: 114. -
Ramos-Sevillano, E. et al. (2016) PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.
PLoS Pathog. 12 (3): e1005500. -
Abbate, F. et al. (2016) Acid-sensing ion channel immunoreactivities in the cephalic neuromasts of adult zebrafish.
Ann Anat. 207: 27-31. -
Shea, G.K. et al. (2020) Juxtacrine signalling via Notch and ErbB receptors in the switch to fate commitment of bone marrow-derived Schwann cells.
Eur J Neurosci. 52 (5): 3306-21. -
Siqueira, R.F. & Fernandes, R.L. (2018) Cryopreservation of lymphocytes for immunological studies in horses
Pesquisa Veterinária Brasileira. 38 (11): 2019-22. -
Miguelena Chamorro, B. et al. (2023) Characterization of Canine Peyer's Patches by Multidimensional Analysis: Insights from Immunofluorescence, Flow Cytometry, and Single-Cell RNA Sequencing.
Immunohorizons. 7 (11): 788-805.
View The Latest Product References
-
Murugaiah, V. et al. (2024) A transgenic mouse with a humanised B cell repertoire mounts an antibody response to influenza infection and vaccination.
J Infect Dis. Sep 24 [Epub ahead of print].
- RRID
- AB_609693
- UniProt
- P01867
- P01865
- P01863
- P01864
- P01868
- P01869
- P03987
- Entrez Gene
- Igh-1a
- Igh-1a
- Igh-1a
- Ighg2b
- Ighg1
- Ighg1
- AI324046
- GO Terms
- GO:0003823 antigen binding
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0019731 antibacterial humoral response
- View More GO Terms
- GO:0050766 positive regulation of phagocytosis
- GO:0042571 immunoglobulin complex, circulating
- GO:0005576 extracellular region
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
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