IgE antibody
Goat anti Mouse IgE antibody (STAR110) used as a coating reagent in a sandwich ELISA to assess IgE levels in murine serum samples.
Image caption:
a–c Levels of total IgE (a), HDM-specific IgE (b), and HDM-specific IgG (c) 24 h after theanti Mouse last HDM challenge. Results show values from individual mice with bars indicating mean ± SEM from n = 12 mice (PBS/PBS group), n = 16 mice (CRM197/PBS group), n = 19 mice (IL-13-K/HDM group), or n = 20 mice (IL-4-K and IL-4-K + IL-13-K groups) pooled from two independent experiments.
From: Conde E, Bertrand R, Balbino B, Bonnefoy J, Stackowicz J, Caillot N, Colaone F, Hamdi S, Houmadi R, Loste A, Kamphuis JBJ, Huetz F, Guilleminault L, Gaudenzio N, Mougel A, Hardy D, Snouwaert JN, Koller BH, Serra V, Bruhns P, Grouard-Vogel G, Reber LL.
Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice.
Nat Commun. 2021 May 11;12(1):2574.
doi: 10.1038/s41467-021-22834-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Mouse IgE antibody (STAR110P) used as a detection reagent in a sandwich ELISA to evaluate IgE expression in murine serum samples.
Image caption:
Peribronchila inflammation score and immunoglobulin levels in serum. The peribronchial inflammation score and OVA-sIgE levels in OVA group and OVA323-339 group were significantly increased, but they were significantly decreased accompanied by increased OVA-sIgG1 after OVA323-339MAP treatment (* compared with the control group, P <0.05; # compared with the OVA323-339 group, P <0.05). Data represent one of three independent experiments.
From: Su W, Zhong W, Zhang Y, Xia Z.
Synthesized OVA323-339MAP octamers mitigate OVA-induced airway inflammation by regulating Foxp3 T regulatory cells.
BMC Immunol. 2012 Jul 6;13:34.
doi: 10.1186/1471-2172-13-34.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti mouse IgE polyclonal antibody recognizes mouse IgE and shows negligible cross - reactivity with other immunoglobulins.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography using antigen coupled to agarose beads.
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Antiserum Preparation
- Antisera to mouse IgE were raised by repeated immunisations of goats with highly purified antigen.
- Buffer Solution
- 50mM HEPES pH7.1
0.1M NaCl
1.0mM MgCl2
0.1mM ZnCl2 - Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Preservative Stabilisers
- 0.05% Proclin™ 300
0.2% Bovine Serum Albumin - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Native mouse IgE.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P06336
- Entrez Gene
- Gm900
- GO Terms
- GO:0003823 antigen binding
- Acknowledgements
- Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
- Regulatory
- For research purposes only
Applications of IgE antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1000 | 1/10000 | |
| ELISA | 1/100 | 1/1000 | |
| ELISA | 1/100 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgE antibody
-
Xia,Z.W. et al (2007) Heme oxygenase-1 attenuates ovalbumin-induced airway inflammation by up-regulation of foxp3 T-regulatory cells, interleukin-10, and membrane-bound transforming growth factor- 1.
Am J Pathol. 171: 1904-14. -
Hartmann, W. et al. (2011) A novel and divergent role of granzyme a and B in resistance to helminth infection.
J Immunol. 186: 2472-81. -
Hadzhieva, M. et al. (2015) Mechanism and functional implications of the heme-induced binding promiscuity of IgE.
Biochemistry. 54 (11): 2061-72. -
Su, W. et al. (2012) Synthesized OVA323-339MAP octamers mitigate OVA-induced airway inflammation by regulating Foxp3 T regulatory cells.
BMC Immunol. 13: 34. -
Conde, E. et al. (2021) Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice.
Nat Commun. 12 (1): 2574. -
Mai, H.L. et al. (2020) Targeting the interleukin-7 receptor alpha by an anti-CD127 monoclonal antibody improves allergic airway inflammation in mice.
Clin Exp Allergy. 50 (7): 824-834.
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