IgA antibody

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Goat anti Mouse IgA:Alk. Phos.

Goat anti Mouse IgA:Biotin

Goat anti Mouse IgA:RPE

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
104004 E 1 ml
104008 E IB IF 1 mg
104009 F 0.25 mg
Goat anti Mouse IgA antibody recognizes murine IgA as demonstrated by ELISA, FLISA and/or Flow cytometry.
Goat anti Mouse IgA antibody has been cross adsorbed against mouse IgG1, IgG2a, IgG2b, IgG3 and IgM.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Ig Fraction conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Antiserum Preparation
Antiserum to mouse IgA was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
50% Glycerol
Preservative Stabilisers
0.1% Sodium azide
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Stabilizing agent
Approx. Protein Concentrations
0.5 mg/ml
Approx. Protein Concentrations
0.25 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
+4ºC, -20ºC if preferred
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.
Shelf Life
18 months from the date of despatch.
Shelf Life
18 months from the date of despatch.

More Information

UniProt
P01878 Related reagents
Entrez Gene
Igh-VJ558 Related reagents
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/4000
ELISA
Immunoblotting
Immunofluorescence
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Application Based External Images

ELISA

Product Specific References

References for IgA antibody

  1. Lindner, C. et al. (2012) Age, microbiota, and T cells shape diverse individual IgA repertoires in the intestine.
    J Exp Med. 209: 365-77.
  2. Tregoning, J.S. et al. (2013) A "prime-pull" vaccine strategy has a modest effect on local and systemic antibody responses to HIV gp140 in mice.
    PLoS One. 8: e80559.