IgA antibody

Goat anti Mouse IgA:Alk. Phos.

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgA

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104004
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Goat anti Mouse IgA antibody recognizes murine IgA as demonstrated by ELISA, FLISA and/or Flow cytometry.
Goat anti Mouse IgA antibody has been cross adsorbed against mouse IgG1, IgG2a, IgG2b, IgG3 and IgM.

Target Species
Mouse
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Antiserum Preparation
Antiserum to mouse IgA was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
50% Glycerol
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/4000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IgA antibody

  1. Lindner, C. et al. (2012) Age, microbiota, and T cells shape diverse individual IgA repertoires in the intestine.
    J Exp Med. 209: 365-77.
  2. Tregoning, J.S. et al. (2013) A "prime-pull" vaccine strategy has a modest effect on local and systemic antibody responses to HIV gp140 in mice.
    PLoS One. 8: e80559.

ELISA

RRID
AB_619826
UniProt
P01878
Entrez Gene
Igh-VJ558
GO Terms
GO:0003823 antigen binding
View more products with IGA specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products