FLIP L antibody
Rabbit anti FLIP antibody recognizes the apoptosis related protein designated FLIP (FLICE inhibitory protein).
FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1.
FLIP exists in 2 forms, FLIPs (short) and FLIPL (long). Rabbit anti FLIP antibody (AHP481) recognizes only FLIPL.
FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1.
FLIP exists in 2 forms, FLIPs (short) and FLIPL (long). Rabbit anti FLIP antibody (AHP481) recognizes only FLIPL.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to murine FLIP were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- Synthetic peptide from C-terminus of murine FLIPL.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O35732
- Entrez Gene
- Cflar
- GO Terms
- GO:0005515 protein binding
- GO:0004197 cysteine-type endopeptidase activity
- GO:0005737 cytoplasm
- GO:0006508 proteolysis
- GO:0043066 negative regulation of apoptosis
- GO:0043154 negative regulation of caspase activity
- Regulatory
- For research purposes only
Applications of FLIP L antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunocytochemistry | 5 ug/ml | ||
| Western Blotting | 1/500 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for FLIP L antibody
-
Zhang, J. et al. (2001) Impaired Fas signaling pathway is involved in defective T cell apoptosis in autoimmune murine arthritis.
J Immunol. 166: 4981-6.
Further Reading
-
Irmler, M. et al. (1997) Inhibition of death receptor signals by cellular FLIP.
Nature. 388 (6638): 190-5.
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