FLIP L antibody
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WB IC ResetRabbit anti Mouse FLIP (C-Terminal)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- FLIP L
- Region
- (C-TERMINAL)
Antibody Quality and 
Unrivaled 
| Rabbit anti FLIP antibody recognizes the apoptosis related protein designated FLIP (FLICE inhibitory protein). FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1. FLIP exists in 2 forms, FLIPs (short) and FLIPL (long). Rabbit anti FLIP antibody (AHP481) recognizes only FLIPL. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to murine FLIP were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- Synthetic peptide from C-terminus of murine FLIPL.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunocytochemistry |  |
5 ug/ml | |
| Western Blotting | |
1/500 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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References for FLIP L antibody
-
Zhang, J. et al. (2001) Impaired Fas signaling pathway is involved in defective T cell apoptosis in autoimmune murine arthritis.
J Immunol. 166: 4981-6.
Further Reading
-
Irmler, M. et al. (1997) Inhibition of death receptor signals by cellular FLIP.
Nature. 388 (6638): 190-5.
- RRID
- AB_2276277
- UniProt
- O35732
- Entrez Gene
- Cflar
- GO Terms
- GO:0005515 protein binding
- GO:0004197 cysteine-type endopeptidase activity
- GO:0005737 cytoplasm
- GO:0006508 proteolysis
- GO:0043066 negative regulation of apoptosis
- GO:0043154 negative regulation of caspase activity
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