FLIP L antibody

Rabbit anti Mouse FLIP (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
FLIP L
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP481
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IC WB 0.1 mg loader
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loader
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Rabbit anti FLIP antibody recognizes the apoptosis related protein designated FLIP (FLICE inhibitory protein).

FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1.

FLIP exists in 2 forms, FLIPs (short) and FLIPL (long). Rabbit anti FLIP antibody (AHP481) recognizes only FLIPL.

Target Species
Mouse
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to murine FLIP were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide from C-terminus of murine FLIPL.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunocytochemistry 5 ug/ml
Western Blotting 1/500 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for FLIP L antibody

  1. Zhang, J. et al. (2001) Impaired Fas signaling pathway is involved in defective T cell apoptosis in autoimmune murine arthritis.
    J Immunol. 166: 4981-6.

Further Reading

  1. Irmler, M. et al. (1997) Inhibition of death receptor signals by cellular FLIP.
    Nature. 388 (6638): 190-5.

RRID
AB_2276277
UniProt
O35732
Entrez Gene
Cflar
GO Terms
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0005737 cytoplasm
GO:0006508 proteolysis
GO:0043066 negative regulation of apoptosis
GO:0043154 negative regulation of caspase activity
View more products with FLIP specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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