FLIP L antibody

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Rabbit anti Mouse FLIP (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP481
Datasheet
IC WB
MSDS
0.1 mg loader

Rabbit anti FLIP antibody recognizes the apoptosis related protein designated FLIP (FLICE inhibitory protein).

FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1.

FLIP exists in 2 forms, FLIPs (short) and FLIPL (long). Rabbit anti FLIP antibody (AHP481) recognizes only FLIPL.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to murine FLIP were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide from C-terminus of murine FLIPL.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O35732
Entrez Gene
Cflar
GO Terms
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0005737 cytoplasm
GO:0006508 proteolysis
GO:0043066 negative regulation of apoptosis
GO:0043154 negative regulation of caspase activity
Regulatory
For research purposes only

Applications of FLIP L antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunocytochemistry 5 ug/ml
Western Blotting 1/500 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for FLIP L antibody

  1. Zhang, J. et al. (2001) Impaired Fas signaling pathway is involved in defective T cell apoptosis in autoimmune murine arthritis.
    J Immunol. 166: 4981-6.

Further Reading

  1. Irmler, M. et al. (1997) Inhibition of death receptor signals by cellular FLIP.
    Nature. 388 (6638): 190-5.