Collagen IV antibody























Mutations in collagen IV genes have been implicated in inherited nephropathies and potentially in cystic kidney disease and intracranial aneurysms (Plaisier et al. 2007).
Rabbit amti Mouse Collagen IV antibody has been successfully employed for the detection of collagen IV by immunofluorescence and immunohistochemistry in mice (Tang et al. 2010), rats (Shen et al. 2014) and oragutan (Bredies et al. 2013).
The following cross reactivities have been observed:
Mouse type IV | 100% |
Mouse types I, II & III | <0.1% |
Human types IV & V | <0.1% |
Mouse fibronectin & laminin | <0.1% |
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Orangutan Rat Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified Ig - liquid
- Preparation
- Purified IgG prepared by antigen column chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
Antibiotic antimycotic mixture 1%0.09% Sodium Azide (NaN3) - Immunogen
- Collagen IV purified from mouse EHS tumor.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P02463
- P08122
- Q9QZS0
- Q9QZR9
- Q80V57
- Q6PFB1
- Entrez Gene
- Col4a1
- Col4a2
- Col4a4
- Col4a3
- GO Terms
- GO:0001525 angiogenesis
- GO:0005201 extracellular matrix structural constituent
- GO:0005587 collagen type IV
- GO:0007528 neuromuscular junction development
- GO:0005488 binding
- GO:0032836 glomerular basement membrane development
- GO:0007155 cell adhesion
- GO:0005178 integrin binding
- GO:0006917 induction of apoptosis
- GO:0006919 activation of caspase activity
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0008283 cell proliferation
- GO:0016525 negative regulation of angiogenesis
- Regulatory
- For research purposes only
Applications of Collagen IV antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/2000 | ||
Immunofluorescence | 1/40 | ||
Immunohistology - Frozen | 1/500 | ||
Immunohistology - Paraffin | 1/500 |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg | ||
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for Collagen IV antibody
-
Tang, Z. et al. (2010) Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
J Exp Med. 207: 867-80. -
Fantin, A. et al. (2010) Tissue macrophages act as cellular chaperones for vascular anastomosis downstream of VEGF-mediated endothelial tip cell induction.
Blood.116: 829-40. -
Shen, W. et al. (2014) Systemic Administration of Erythropoietin Inhibits Retinopathy in RCS Rats.
PLoS One. 9: e104759. -
Bredies, K. et al. (2013) Computer-assisted counting of retinal cells by automatic segmentation after TV denoising.
BMC Ophthalmol. 13: 59. -
Rubin, A.N. et al. (2010) The germinal zones of the basal ganglia but not the septum generate GABAergic interneurons for the cortex.
J Neurosci. 30: 12050-62. -
Weinl, C. et al. (2014) Elk3 deficiency causes transient impairment in post-natal retinal vascular development and formation of tortuous arteries in adult murine retinae.
PLoS One. 9: e107048. -
Stenzel, D. et al. (2011) Integrin-dependent and -independent functions of astrocytic fibronectin in retinal angiogenesis.
Development. 138: 4451-63. -
Zuercher, J. et al. (2012) Norrin stimulates cell proliferation in the superficial retinal vascular plexus and is pivotal for the recruitment of mural cells.
Hum Mol Genet. 21: 2619-30. -
Arnold, T.D. et al. (2012) Defective retinal vascular endothelial cell development as a consequence of impaired integrin αVβ8-mediated activation of transforming growth factor-β.
J Neurosci. 32: 1197-206. -
Chen, M. et al. (2010) Immune activation in retinal aging: a gene expression study.
Invest Ophthalmol Vis Sci. 51: 5888-96. -
Kojima, T. et al. (2007) Proangiogenic role of ephrinB1/EphB1 in basic fibroblast growth factor-induced corneal angiogenesis.
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Ma, S. et al. (2012) Ric-8a, a Guanine Nucleotide Exchange Factor for Heterotrimeric G Proteins, Regulates Bergmann Glia-Basement Membrane Adhesion during Cerebellar Foliation.
J Neurosci. 32: 14979-93. -
Dulauroy, S. et al. (2012) Lineage tracing and genetic ablation of ADAM12(+) perivascular cells identify a major source of profibrotic cells during acute tissue injury.
Nat Med. 18: 1262-70. -
Edgar, K. et al. (2012) eNOS Overexpression Exacerbates Vascular Closure in the Obliterative Phase of OIR and Increases Angiogenic Drive in the Subsequent Proliferative Stage.
Invest Ophthalmol Vis Sci. 53: 6833-50. -
Lange, C.A. et al. (2012) Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.
Development. 139: 2340-50. -
Li, W. and Mukouyama, Y.S. (2011) Whole-mount immunohistochemical analysis for embryonic limb skin vasculature: a model system to study vascular branching morphogenesis in embryo.
J Vis Exp. 51: pii: 2620. -
Lutter, S. et al. (2012) Smooth muscle-endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation.
J Cell Biol. 197: 837-49. -
McKenzie, J.A. et al. (2012) Apelin is required for non-neovascular remodeling in the retina.
Am J Pathol. 180: 399-409. -
Powner, M.B. et al. (2012) Visualization of gene expression in whole mouse retina by in situ hybridization.
Nat Protoc. 7: 1086-96. -
Schulz, C. et al. (2012) A lineage of myeloid cells independent of Myb and hematopoietic stem cells.
Science. 336: 86-90. -
Scott, A. et al. (2010) Astrocyte-derived vascular endothelial growth factor stabilizes vessels in the developing retinal vasculature.
PLoS One. 5: e11863. -
Takagi, N. et al. (2012) Mineralocorticoid Receptor Blocker Protects against Podocyte-Dependent Glomerulosclerosis.
Nephron Extra. 2: 17-26. -
Chen, M. et al. (2013) Age- and light-dependent development of localised retinal atrophy in CCL2(-/-)CX3CR1(GFP/GFP) mice.
PLoS One. 8: e61381. -
Luhmann UF. et al. (2005) Berger W. Role of the Norrie disease pseudoglioma gene in sprouting angiogenesis
during development of the retinal vasculature.
Invest Ophthalmol Vis Sci. 46: 3372-82. -
Scott, A. et al. (2014) Quantification of vascular tortuosity as an early outcome measure in oxygen induced retinopathy (OIR)
Exp Eye Res. 120: 55-60. -
Yukiura H et al. (2015) Autotaxin overexpression causes embryonic lethality and vascular defects.
PLoS One. 10 (5): e0126734. -
Williams, J.A. et al. (2016) Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina.
PLoS One. 11 (8): e0161898. -
Piñero G et al. (2016) Lithium Reversibly Inhibits Schwann Cell Proliferation and Differentiation Without Inducing Myelin Loss.
Mol Neurobiol. Dec 5. [Epub ahead of print] -
Gurnik, S. et al. (2016) Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling.
Acta Neuropathol. 131 (5): 753-73. -
Misra, A. et al. (2016) Integrin β3 inhibition is a therapeutic strategy for supravalvular aortic stenosis.
J Exp Med. 213 (3): 451-63. -
Wu, W.K. et al. (2015) IL-4 regulates specific Arg-1(+) macrophage sFlt-1-mediated inhibition of angiogenesis.
Am J Pathol. 185 (8): 2324-35. -
Toffoli, B. et al. (2017) Nephropathy in Pparg-null mice highlights PPARγ systemic activities in metabolism and in the immune system.
PLoS One. 12 (2): e0171474. -
Yanagida, K. et al. (2017) Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1.
Proc Natl Acad Sci U S A. 114 (17): 4531-6. -
Fernández-robredo, P. et al. (2017) Neuropilin 1 Involvement in Choroidal and Retinal Neovascularisation.
PLoS One. 12 (1): e0169865. -
Kim, B. et al. (2018) Endothelial pyruvate kinase M2 maintains vascular integrity.
J Clin Invest. 128 (10): 4543-56. -
Villard, O. et al. (2020) Characterization of immortalized human islet stromal cells reveals a MSC-like profile with pancreatic features.
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Chu, C.J. et al. (2013) Assessment and in vivo scoring of murine experimental autoimmune uveoretinitis using optical coherence tomography.
PLoS One. 8 (5): e63002. -
Krivanek, J. et al. (2020) Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 11 (1): 4816.
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