Collagen IV antibody

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Anti Mouse Collagen IV Antibody thumbnail image 13

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Anti Mouse Collagen IV Antibody thumbnail image 16

Anti Mouse Collagen IV Antibody thumbnail image 17

Anti Mouse Collagen IV Antibody thumbnail image 18

Anti Mouse Collagen IV Antibody thumbnail image 19

Anti Mouse Collagen IV Antibody thumbnail image 20

Anti Mouse Collagen IV Antibody thumbnail image 21

Anti Mouse Collagen IV Antibody thumbnail image 22

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Anti Mouse Collagen IV Antibody thumbnail image 24

Anti Mouse Collagen IV Antibody thumbnail image 25

Anti Mouse Collagen IV Antibody gallery image 1 — **Published customer image:**
Rabbit anti Mouse collagen 4 antibody (**2150-1470**) used for detection of collagen 4 in rat retina by immunofluorescence on cryostat sections.
**Image caption:**
Retinal gliosis and subretinal neovascularization in RCS rats. Double label immunostaining was performed using antibodies against vimentin (red) and collagen type IV (green) on frozen sections. (A) Non-dystrophic rat at 21 weeks (w) of age showed a normal distribution of retinal vessels confined to the inner retina and absence of glial cells in the subretinal space. (B, C) RCS rats at 6 w (B) and 9 w (C) of age showed activation of Muller cells but without subretinal neovascularization. Muller cell invasion into the subretinal space was more obvious at 9 w compared with 6 w of age (arrows in C). (D) Subretinal neovascularization (arrows) in RCS rats at 13 w of age. GCL = ganglion cell layer, INL = inner nuclear layer, OPL = outer plexiform layer, ONL = outer nuclear layer, SP = subretinal space, CC = choroidal capillaries. Scale bars: 50 μm.

From: Shen W, Chung SH, Irhimeh MR, Li S, Lee S-R, *et al*. (2014)
Systemic Administration of Erythropoietin Inhibits Retinopathy in RCS Rats.
PLoS ONE 9(8): e104759.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 2 — **Published customer image:**Rabbit anti Mouse collagen 4 antibody (**2150-1470**) used for the detection of collogen 4 expression in Elk3 knockout mice by immunofluorescence.
**Image caption:**
Smooth muscle actin and CollagenIV levels are not altered in Elk3(−/−) knockout retinae. (A) IsolectinB4 (ILB4) (left) and smooth muscle actin (SMA) (middle and right) staining of retinal flat-mounts of control (upper panel) and Elk3(−/−) knockout animals. (B) IsolectinB4 (left) and collagenIV (middle and right) staining of retinal flat-mounts of control (upper panel) and Elk3(−/−) knockout animals. (C) Quantitation of collagenIV RNA levels in control and Elk3(−/−) knockout retinae (n = 5 independent experiments). (D) Representative pairs of control and Elk3(−/−) knockout retinal lysates tested for smooth muscle actin expression in Western Blot analysis. (E) Quantitation of control and Elk3(−/−) knockout retinal levels of SMA (n = 5 independent experiments). Scale bar in (A, B left and middle) 1000 μm, in (A, B right) 100 μm.

From: Weinl C, Wasylyk C, Garcia Garrido M, Sothilingam V, Beck SC, *et a*l. (2014)
Elk3 Deficiency Causes Transient Impairment in Post-Natal Retinal Vascular Development and Formation of Tortuous Arteries in Adult Murine Retinae.
PLoS ONE 9(9): e107048.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 3 — **Published customer image:**Rabbit anti Mouse collagen IV antibody (**2150-1470**) used for the evaluation of collagen IV expression in murine pupillary membranes by immunofluorescence on cryosections.
**Image caption:**
Vhltrp1-creKO mice demonstrate a Hif1a-dependent persistence of the pupillary membrane. (A-I) Autofluorescence scanning laser ophthalmoscope (AF-SLO) imaging (A,D,G), indocyanin-green angiography (ICGA; B,E,H) and collagen IV-stained cryosections (C,F,I) of Vhltrp1-creKO, Vhl;Hif1atrp1-creKO and control littermates at P21 show a persistent pupillary membrane in Vhltrp1-creKO mice that is absent in Vhl;Hif1atrp1-creKO and control mice. CB, ciliary body. Scale bar: 75 μm.

From: Lange CA, Luhmann UF, Mowat FM, Georgiadis A, West EL, Abrahams S, Sayed H, Powner MB, Fruttiger M, Smith AJ, Sowden JC, Maxwell PH, Ali RR, Bainbridge JW.
Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.
Development. 2012 Jul;139(13):2340-50.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 4 — **Published customer image:**
Rabbit anti Mouse collagen IV antibody (**2150-1470**) used for the evaluation of collagen IV expression in retinal flat-mounts by immunofluorescence.
**Image caption:**
Vhltrp1-creKO mice show a persistence of the hyaloid vasculature and a disorganised retinal vasculature that is independent of Hif1a. (A,B,F,G,K,L) Indocyanin-green angiography (A,F,K) and corresponding collagen 4-stained retinal flatmounts (B,G,L) show a persistent hyaloid vasculature in Vhltrp1-creKO and Vhl;Hif1atrp1-creKO mice that is absent in control littermates at P21. Inset in K shows angiography focussed on the persistent hyaloid vasculature. Inset in L shows a light microscopy image of a retinal flat mount with haemorrhage. (C,H,M) Vhltrp1-creKO and Vhl;Hif1atrp1-creKO mice demonstrate a disorganised retinal vasculature with areas of intra-retinal vascular proliferation compared with age-matched controls (hyaloid vessels were removed). Outlined regions indicate the areas selected for IMARES imaging. (D,E,I,J,N,O) IMARES reconstruction reveals a disorganised retinal vasculature with abrogated vascular layering and retinal vessels growing into the outer retina in Vhltrp1-creKO and Vhl;Hif1atrp1-creKO mice. Scale bars: 1 mm for B; 500 μm for C,M; 700 μm for H; 50 μm for D,E,I,J,N,O.

From: Lange CA, Luhmann UF, Mowat FM, Georgiadis A, West EL, Abrahams S, Sayed H, Powner MB, Fruttiger M, Smith AJ, Sowden JC, Maxwell PH, Ali RR, Bainbridge JW.
Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.
Development. 2012 Jul;139(13):2340-50.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 5 — **Published customer image:**
Rabbit anti Mouse collagen IV antibody (**2150-1470**) used for the detection of collagen IV in mouse retina by immunofluorescence.
**Image caption:**
Deletion of Vhl in the RPE results in disruption of the retinal vascular architecture and formation of chorioretinal anastomoses. (A-L) Representative collagen IV-stained cryosections of control (A-D), Vhltrp1-creKO mice (E-H) and Vhl;Hif1atrp1-creKO (I-L) and at different time points during development show a disorganised retinal vasculature and the formation of chorioretinal anastomoses in Vhl;Hif1atrp1-creKO and Vhltrp1-creKO mice (G,K). GCL, ganglion cell layer; NBL, neuroblast layer; Cho, choroid; INL, inner nuclear layer, ONL, outer nuclear layer. Scale bars: 25 μm.

From: Lange CA, Luhmann UF, Mowat FM, Georgiadis A, West EL, Abrahams S, Sayed H, Powner MB, Fruttiger M, Smith AJ, Sowden JC, Maxwell PH, Ali RR, Bainbridge JW.
Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.
Development. 2012 Jul;139(13):2340-50.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 6 — **Published customer image:**
Rabbit anti Murine cytokeratin 4 antibody (**2150-1470**) used for the identification of collagen IV expressing vasculature in mouse brains by immunofluorescence on vibratome sections.
**Image caption:**
Immunohistochemistry analysis of pericytes in Ang-2 GOF mice. a Pericytes (desmin+) were decreased in GOF mice utilizing 10 μm cryosections for analysis (n = 8, scale bars 25μm). b 50 μm vibratome sections of Ang-2 GOF and WT mice revealed decrease in pericyte area/vessel area [%] (n = 3, scale bars 10 μm) indicating decreased pericyte coverage of the vessels in GOF mice

From: Gurnik S, Devraj K, Macas J, Yamaji M, Starke J, Scholz A, Sommer K, Di Tacchio M, Vutukuri R, Beck H, Mittelbronn M, Foerch C, Pfeilschifter W, Liebner S, Peters KG, Plate KH, Reiss Y.
Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling.
Acta Neuropathol. 2016 May;131(5):753-73.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 7 — **Published customer image:**
Rabbit anti Human collagen IV antibody (**2150-1470**) showing increased renal collagen IV deposition in aged Pparg^Δ/Δ mice over controls by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
**Image caption:**
Immunohistochemistry and gene expression of fibrosis markers in kidney of ageing Pparg^Δ/Δ mice, and plasmatic levels of anti-β2-glycoprotein 1 antibodies.
52 weeks old control (CTL) and Ppar^Δ/Δ (γ^Δ/Δ) animals were analyzed for the following parameters: (A) Top panels: Kidney Immunohistochemistry for collagen IV (Col-IV). Positive staining is in brown. Scale bar represents 100μm.

From: Toffoli B, Gilardi F, Winkler C, Soderberg M, Kowalczuk L, Arsenijevic Y, *et al.* (2017)
Nephropathy in Pparg-null mice highlights PPARγ systemic activities in metabolism and in the immune system.
PLoS ONE 12(2): e0171474.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 8 — **Published customer image:**
Rabbit anti Human collagen IV antibody (**2150-4140**) used for the evaluation of collagen IV expression in human stem cell preparations by western blotting.
**Image caption:**
hISCs secrete pancreatic ECM proteins. Extracted proteins from hISCs and BM-MSCs and corresponding conditioned media were analyzed by western blotting. Type I, IV, and VI collagens as well as fibronectin and laminin were detected in both cell types but at different levels of expression. β-actin was used as loading control. The right panels represent the quantification of the Western blot, n = 4, *p <0.05, significant differences

From: Villard O, Armanet M, Couderc G, *et al*.
Characterization of immortalized human islet stromal cells reveals a MSC-like profile with pancreatic features.
Stem Cell Res Ther. 2020;11(1):158. doi:10.1186/s13287-020-01649-z
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 9 — **Published customer image:**
Rabbit anti Mouse collagen IV antibody (**2150-1470**) used to demonstrate blood vessels by immunofluorescence.
**Image caption:**
OCT identifies vascular changes in EAU and appearances are altered by depth and degree of infiltration.
The vessel (collagen IV in red) with altered OCT signal in the perivascular tissue (upward pointing arrowheads) is densely infiltrated with myeloid - lectin B4 binding (white) and T cell - CD4+ & CD8+ (green) cells as shown by immunohistochemistry on retinal flat mount (H). This is in contrast to the other vessel (downward pointing arrowheads), which lacks any localised infiltrate. Diffuse hyper-reflective signal on OCT (I) corresponds to a deep retinal vein, with associated cellular infiltrate (J). The blue line indicates alignment with the OCT scan.

From: Chu CJ, Herrmann P, Carvalho LS, Liyanage SE, Bainbridge JWB, Ali RR, *et al*. (2013)
Assessment and *In Vivo* Scoring of Murine Experimental Autoimmune Uveoretinitis Using Optical Coherence Tomography.
PLoS ONE 8(5): e63002.
doi: 10.1371/journal.pone.0063002
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 10 — **Published customer image:**
Rabbit anti Mouse collagen IV antibody (**2150-1470**) used to demonstrate blood vessels by immunofluorescence.
**Image caption:**
OCT imaging can guide identification and characterisation of novel features within the EAU model.
Retinal flat-mount revealed an atypical vascular lesion arising along the course of a large vessel (B). 3D reconstruction of the region enclosed by the white box, with CD4+ & CD8+ cells (green) displayed, collagen IV (red) (C).

From: Chu CJ, Herrmann P, Carvalho LS, Liyanage SE, Bainbridge JWB, Ali RR, *et al*. (2013)
Assessment and *In Vivo* Scoring of Murine Experimental Autoimmune Uveoretinitis Using Optical Coherence Tomography.
PLoS ONE 8(5): e63002.
doi: 10.1371/journal.pone.0063002
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 11 — **Published customer image:**
Rabbit anti Mouse Collagen IV antibody (**2150-1470**) used to label blood vessels in mouse dental tissue by immunofluorescence.
**Image caption:**
Unbiased identification, validation, and spatial mapping of major dental cell types and subpopulations.
Validations and mapping of unbiasedly identified populations based on the expression of selected marker genes. Note. COL4 is a well known marker for blood vessels, this marker genes is highly and specifically expressed in corresponding clusters (Supplementary Table 1), but do not belong to top10 genes shown in plots above the images. (LiCL Lingual Cervical Loop, LaCL Labial Cervical Loop, SI Stratum Intermedium, SR Stellate reticulum, OEE Outer Enamel Epithelium). Scare bars: 50 μm.

From: Krivanek J, *et al*.
Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 2020 Sep 23;11(1):4816.
doi: 10.1038/s41467-020-18512-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 12 — **Published customer image:**
Rabbit anti Mouse Collagen IV antibody (**2150-1470**) used to label blood vessels in mouse dental tissue by immunofluorescence.
**Image caption:**
Heterogeneity of immune cells in mouse incisor.
c Location of tissue-residential immune cells in the different parts of mouse incisor. **AIF1+ macrophages are located in the whole incisor including apical pulp, cervical loop, odontoblast layer, and distal pulp** in contrast to LYVE+ macrophages which mostly reside in the middle part of the pulp, but not inside the odontoblast layer. DPP4+ immune cells are sporadically located in the apical part of the tooth and odontoblast layer. COL4 immunohistochemical staining visualize the blood vessels. (Od. Odontoblasts), Scale bars: 50 μm.

From: Krivanek J, *et al*.
Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 2020 Sep 23;11(1):4816.
doi: 10.1038/s41467-020-18512-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 13 — **Published customer image:**
Rabbit anti Mouse Collagen IV antibody (**2150-1470**) used to label blood vessels in mouse dental tissue by immunofluorescence.
**Image caption:**
Heterogeneity of immune cells in mouse incisor.
c Location of tissue-residential immune cells in the different parts of mouse incisor. AIF1+ macrophages are located in the whole incisor including apical pulp, cervical loop, odontoblast layer, and distal pulp in contrast to **LYVE+ macrophages which mostly reside in the middle part of the pulp, but not inside the odontoblast layer**. DPP4+ immune cells are sporadically located in the apical part of the tooth and odontoblast layer. COL4 immunohistochemical staining visualize the blood vessels. (Od. Odontoblasts), Scale bars: 50 μm.

From: Krivanek J, *et al*.
Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 2020 Sep 23;11(1):4816.
doi: 10.1038/s41467-020-18512-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 14 — **Published customer image:**
Rabbit anti Mouse Collagen IV antibody (**2150-1470**) used to label blood vessels in mouse dental tissue by immunofluorescence.
**Image caption:**
Heterogeneity of immune cells in mouse incisor.
c Location of tissue-residential immune cells in the different parts of mouse incisor. AIF1+ macrophages are located in the whole incisor including apical pulp, cervical loop, odontoblast layer, and distal pulp in contrast to LYVE+ macrophages which mostly reside in the middle part of the pulp, but not inside the odontoblast layer. **DPP4+ immune cells are sporadically located in the apical part of the tooth and odontoblast layer**. COL4 immunohistochemical staining visualize the blood vessels. (Od. Odontoblasts), Scale bars: 50 μm.

From: Krivanek J, *et al*.
Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 2020 Sep 23;11(1):4816.
doi: 10.1038/s41467-020-18512-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 15 — **Published customer image:**
Rabbit anti Mouse Collagen IV antibody (**2150-1470**) used to label blood vessels in mouse dental tissue by immunofluorescence.
**Image caption:**
Identification of previously unrecognized cell types in dental epithelium and stem cells.
g, h Immunohistochemistry identification of the Cuboidal layer of stratum intermedium (expressing THBD) and spatial relation to the neighboring blood vessels submerged into the papillary structure of stratum intermedium. COL4 expression characterizes the blood vessels on left panel. h Papillary structure of stratum intermedium with submerged blood vessels (COL4) and CDH1 expressing ameloblasts and cells from stratum intermedium. Note. Cuboidal layer characterized by THBD expression (g) forms subpopulation of stratum intermedium cells (h). Immunohistochemistry. (am. ameloblasts, od. odontoblasts, LaCL Labial Cervical Loop, SI stratum intermedium, OEE Outer Enamel Epithelium, am. Ameloblasts, PDL periodontal ligamentum). Scale bars: 50 μm.

From: Krivanek J, *et al*.
Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 2020 Sep 23;11(1):4816.
doi: 10.1038/s41467-020-18512-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 16 — **Published customer image:**
Rabbit anti Mouse Collagen IV antibody (**2150-1470**) used to label blood vessels in mouse dental tissue by immunofluorescence.
**Image caption:**
Cross-section through P3 mandible showing the differential spatial distribution of **AIF1+** and LYVE1+ macrophages (immunohistochemistry). **COL4 immunohistochemical staining to visualize the blood vessels**.

From: Krivanek J, *et al*.
Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 2020 Sep 23;11(1):4816.
doi: 10.1038/s41467-020-18512-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 17 — **Published customer image:**
Rabbit anti Mouse Collagen IV antibody (**2150-1470**) used to label blood vessels in mouse dental tissue by immunofluorescence.
**Image caption:**
Cross-section through P3 mandible showing the differential spatial distribution of AIF1+ and **LYVE1+** macrophages (immunohistochemistry). **COL4 immunohistochemical staining to visualize the blood vessels**.

From: Krivanek J, *et al*.
Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 2020 Sep 23;11(1):4816.
doi: 10.1038/s41467-020-18512-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 18 — **Published customer image:**
Rabbit anti Mouse Collagen IV antibody (**2150-1470**) used to label blood vessels in mouse dental tissue by immunofluorescence.
**Image caption:**
Immunohistochemistry-based validation of AIF1+, LYVE1+ macrophages inside themouse incisor. Together with the figure 5c these panels prove the different histological positioning of two different kind of macrophages (LYVE1+ and LYVE1-). AIF1+ macrophages are located in the whole incisor including apical pulp, cervical loop, odontoblast layer and distal pulp in contrast to LYVE+ macrophages which mostly resides in the middle part of the pulp, but not inside the odontoblast layer. **COL4 immunohistochemical staining to visualize the blood vessels**.

From: Krivanek J, *et al*.
Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 2020 Sep 23;11(1):4816.
doi: 10.1038/s41467-020-18512-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 19 — **Published customer image:**
Rabbit anti Mouse Collagen IV antibody (**2150-1470**) used to label blood vessels in mouse dental tissue by immunofluorescence.
**Image caption:**
Immunohistochemistry-based validation of the NK-cells cluster using DPP4 marker. Position of DPP4+ cells outside the blood vessels in the tooth was proved in 5 independent animals. **COL4 immunohistochemical staining visualize the blood vessels**.

From: Krivanek J, *et al*.
Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 2020 Sep 23;11(1):4816.
doi: 10.1038/s41467-020-18512-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 20 — **Published customer image:**
Rabbit anti Mouse Collagen IV antibody (**2150-1470**) used to label blood vessels in human dental tissue by immunofluorescence.
**Image caption:**
Two different types of macrophages (LYVE1+ and LYVE1-) demonstrate spatially restricted distribution also in human molar (immunohistochemistry). **COL4 immunohistochemical staining visualize the blood vessels**. Scale bars: 50 μm.

From: Krivanek J, *et al*.
Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 2020 Sep 23;11(1):4816.
doi: 10.1038/s41467-020-18512-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 21 — **Published customer image:**
Rabbit anti Mouse Collagen IV antibody (**2150-1470**) used to label blood vessels in human dental tissue by immunofluorescence.
**Image caption:**
Two different types of macrophages (LYVE1+ and LYVE1-) demonstrate spatially restricted distribution also in human molar (immunohistochemistry). **COL4 immunohistochemical staining visualize the blood vessels**. Scale bars: 50 μm.

From: Krivanek J, *et al*.
Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 2020 Sep 23;11(1):4816.
doi: 10.1038/s41467-020-18512-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 22 — **Published customer image:**
Rabbit anti Mouse collagen IV antibody (**2150-1470**) used to highlight collagen in relation to microvessels in mouse brain by immunofluorescence.
**Image caption:**
Reduction of Col IV in TG mice at late age.
Confocal images illustrating Col IV density in relation to the PDCLX⁺ vessels in WT (left panel) and TG mice (right panel).Scale bar: 50 μm. PDCLX = Podocalyxin, Col IV = Collagen IV.

From: Elabi O, Gaceb A, Carlsson R, Padel T, Soylu-Kucharz R, Cortijo I, Li W, Li JY, Paul G.
Human α-synuclein overexpression in a mouse model of Parkinson's disease leads to vascular pathology, blood brain barrier leakage and pericyte activation.
Sci Rep. 2021 Jan 13;11(1):1120.
doi: 10.1038/s41598-020-80889-8..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 23 — **Published customer image:**
Rabbit anti Murine cytokeratin 4 antibody (**2150-1470**) used for the identification of collagen IV expressing vasculature in mouse brains by immunofluorescence on vibratome sections.
**Image caption:**
Immunohistochemistry analysis of pericytes in Ang-2 GOF mice. a Pericytes (desmin+) were decreased in GOF mice utilizing 10 μm cryosections for analysis (n = 8, scale bars 25μm). b 50 μm vibratome sections of Ang-2 GOF and WT mice revealed decrease in pericyte area/vessel area [%] (n = 3, scale bars 10 μm) indicating decreased pericyte coverage of the vessels in GOF mice

From: Gurnik S, Devraj K, Macas J, Yamaji M, Starke J, Scholz A, Sommer K, Di Tacchio M, Vutukuri R, Beck H, Mittelbronn M, Foerch C, Pfeilschifter W, Liebner S, Peters KG, Plate KH, Reiss Y.
Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling.
Acta Neuropathol. 2016 May;131(5):753-73.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 24 — **Published customer image:**
Rabbit anti Mouse collagen IV antibody (**2150-1470**) used to visualize collagen IV expression in murine retina by immunofluorecence.
**Image caption:**
Overexpression of ATX causes abnormal vessel morphology and vessel regression. (A and B) Magnification view of angiogenic front in retina from ATX cTg mice at P6. Control and ATX cTg retinas had similar filopodia protrusion. Scale bar, 50 μm. TM, tamoxifen. (C and D) ATX cTg retinas displayed vessel regression at vascular plexus. Control (wild type) and ATX cTg retinas labeled for CD31 (green) and collagen IV (red). Arrows highlight empty collagen IV sleeves, indicating vessel regression. Scale bar, 100 μm. (E and F) Vessel regression was evaluated quantitatively. Error bars indicate s.d. (control; n = 7, ATX cTg; n = 5). P-values were estimated by student’s t-test, ***P < 0.001. Data were pooled from three independent experiments.

From: Yukiura H, Kano K, Kise R, Inoue A, Aoki J (2015)
Autotaxin Overexpression Causes Embryonic Lethality and Vascular Defects.Citation: Yukiura H, Kano K, Kise R, Inoue A, Aoki J (2015)
Autotaxin Overexpression Causes Embryonic Lethality and Vascular Defects.
PLoS ONE 10(5): e0126734.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Mouse Collagen IV Antibody gallery image 25 — **Published customer image:**
Rabbit anti Mouse collagen IV antibody (**2150-1470**) used to label collagen IV⁺ vessels in mouse retina by immunofluorescence.
**Image caption:**
Endothelial specific deletion of Pkm2 in the post-natal mouse retina.
(d) Representative confocal projections of central plexus vessels in PECAM and Collagen IV stained retinas from control and Pkm2^iEC-KO mice. (e) Quantification of Collagen IV⁺/PECAM⁺ sleeves, ERG⁺ ECs, EC area per field and branch point density at the angiogenic front of control and Pkm2^iEC-KO mice (n=6). e data represent means ± s.e.m. (**P <0.01 by two tailed students t-test) Scale bars in d = 100μm.

From: Stone OA, El-Brolosy M, Wilhelm K, Liu X, Romão AM, Grillo E, Lai JKH, Günther S, Jeratsch S, Kuenne C, Lee IC, Braun T, Santoro MM, Locasale JW, Potente M, Stainier DYR.
Loss of pyruvate kinase M2 limits growth and triggers innate immune signaling in endothelial cells.
Nat Commun. 2018 Oct 9;9(1):4077.
doi: 10.1038/s41467-018-06406-8.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rabbit anti Mouse Collagen IV

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
2150-1470 Datasheet	C E IF P SDS	0.1 ml	Log in		Get Quote

Rabbit anti Mouse Collagen IV antibody recognizes mouse collagen type IV. Collagen IV is a 1682 amino acid ~160 kDa (predicted) matrx protein and major component of glomerular basement membranes. Multiple isoforms exist each capable of forming triple helical structures with two other chains to form the type IV collagen network. The collagen IV alpha chain can be cleaved between residues 1444-1445 to yield the c-terminal 225 amino acid, ~28 kDa arresten fragment, collagen α2(IV) yields a c-terminal canstatin fragment while Collagen α3(IV) yeilds a tumstatin fragment. Collagen IV bears a single collagen IV NC1 (C-terminal non-collagenous) domain (UniProt: Q9QZR9).

Mutations in collagen IV genes have been implicated in inherited nephropathies and potentially in cystic kidney disease and intracranial aneurysms (Plaisier et al. 2007).

Rabbit amti Mouse Collagen IV antibody has been successfully employed for the detection of collagen IV by immunofluorescence and immunohistochemistry in mice (Tang et al. 2010), rats (Shen et al. 2014) and oragutan (Bredies et al. 2013).

The following cross reactivities have been observed:

Mouse type IV	100%
Mouse types I, II & III	<0.1%
Human types IV & V	<0.1%
Mouse fibronectin & laminin	<0.1%

Product Details

Target Species

Mouse

Species Cross-Reactivity

Target Species	Cross Reactivity
Orangutan
Rat
Human

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified Ig - liquid

Preparation

Purified IgG prepared by antigen column chromatography

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Antibiotic antimycotic mixture 1%

Immunogen

Collagen IV purified from mouse EHS tumor.

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P02463; P08122; Q9QZS0; Q9QZR9; Q80V57; Q6PFB1
Entrez Gene: Col4a1; Col4a2; Col4a4; Col4a3
GO Terms: GO:0001525 angiogenesis; GO:0005201 extracellular matrix structural constituent; GO:0005587 collagen type IV; GO:0007528 neuromuscular junction development; GO:0005488 binding; GO:0032836 glomerular basement membrane development; GO:0007155 cell adhesion; GO:0005178 integrin binding; GO:0006917 induction of apoptosis; GO:0006919 activation of caspase activity; GO:0007166 cell surface receptor linked signaling pathway; GO:0008283 cell proliferation; GO:0016525 negative regulation of angiogenesis
Regulatory: For research purposes only

Applications of Collagen IV antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/2000
Immunofluorescence		1/40
Immunohistology - Frozen		1/500
Immunohistology - Paraffin		1/500

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	F IF WB	0.1 mg	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in		Get Quote
100x Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in		Get Quote

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Product Specific References

References for Collagen IV antibody

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Shen, W. et al. (2014) Systemic Administration of Erythropoietin Inhibits Retinopathy in RCS Rats.
PLoS One. 9: e104759.
Bredies, K. et al. (2013) Computer-assisted counting of retinal cells by automatic segmentation after TV denoising.
BMC Ophthalmol. 13: 59.
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J Neurosci. 30: 12050-62.
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PLoS One. 9: e107048.
Stenzel, D. et al. (2011) Integrin-dependent and -independent functions of astrocytic fibronectin in retinal angiogenesis.
Development. 138: 4451-63.
Zuercher, J. et al. (2012) Norrin stimulates cell proliferation in the superficial retinal vascular plexus and is pivotal for the recruitment of mural cells.
Hum Mol Genet. 21: 2619-30.
Arnold, T.D. et al. (2012) Defective retinal vascular endothelial cell development as a consequence of impaired integrin αVβ8-mediated activation of transforming growth factor-β.
J Neurosci. 32: 1197-206.
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Ma, S. et al. (2012) Ric-8a, a Guanine Nucleotide Exchange Factor for Heterotrimeric G Proteins, Regulates Bergmann Glia-Basement Membrane Adhesion during Cerebellar Foliation.
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Dulauroy, S. et al. (2012) Lineage tracing and genetic ablation of ADAM12(+) perivascular cells identify a major source of profibrotic cells during acute tissue injury.
Nat Med. 18: 1262-70.
Edgar, K. et al. (2012) eNOS Overexpression Exacerbates Vascular Closure in the Obliterative Phase of OIR and Increases Angiogenic Drive in the Subsequent Proliferative Stage.
Invest Ophthalmol Vis Sci. 53: 6833-50.
Lange, C.A. et al. (2012) Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.
Development. 139: 2340-50.
Li, W. and Mukouyama, Y.S. (2011) Whole-mount immunohistochemical analysis for embryonic limb skin vasculature: a model system to study vascular branching morphogenesis in embryo.
J Vis Exp. 51: pii: 2620.
Lutter, S. et al. (2012) Smooth muscle-endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation.
J Cell Biol. 197: 837-49.
McKenzie, J.A. et al. (2012) Apelin is required for non-neovascular remodeling in the retina.
Am J Pathol. 180: 399-409.
Powner, M.B. et al. (2012) Visualization of gene expression in whole mouse retina by in situ hybridization.
Nat Protoc. 7: 1086-96.
Schulz, C. et al. (2012) A lineage of myeloid cells independent of Myb and hematopoietic stem cells.
Science. 336: 86-90.
Scott, A. et al. (2010) Astrocyte-derived vascular endothelial growth factor stabilizes vessels in the developing retinal vasculature.
PLoS One. 5: e11863.
Takagi, N. et al. (2012) Mineralocorticoid Receptor Blocker Protects against Podocyte-Dependent Glomerulosclerosis.
Nephron Extra. 2: 17-26.
Chen, M. et al. (2013) Age- and light-dependent development of localised retinal atrophy in CCL2(-/-)CX3CR1(GFP/GFP) mice.
PLoS One. 8: e61381.
Luhmann UF. et al. (2005) Berger W. Role of the Norrie disease pseudoglioma gene in sprouting angiogenesis during development of the retinal vasculature.
Invest Ophthalmol Vis Sci. 46: 3372-82.
Scott, A. et al. (2014) Quantification of vascular tortuosity as an early outcome measure in oxygen induced retinopathy (OIR)
Exp Eye Res. 120: 55-60.
Yukiura H et al. (2015) Autotaxin overexpression causes embryonic lethality and vascular defects.
PLoS One. 10 (5): e0126734.
Williams, J.A. et al. (2016) Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina.
PLoS One. 11 (8): e0161898.
Piñero G et al. (2016) Lithium Reversibly Inhibits Schwann Cell Proliferation and Differentiation Without Inducing Myelin Loss.
Mol Neurobiol. Dec 5. [Epub ahead of print]
Gurnik, S. et al. (2016) Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling.
Acta Neuropathol. 131 (5): 753-73.
Misra, A. et al. (2016) Integrin β3 inhibition is a therapeutic strategy for supravalvular aortic stenosis.
J Exp Med. 213 (3): 451-63.
Wu, W.K. et al. (2015) IL-4 regulates specific Arg-1(+) macrophage sFlt-1-mediated inhibition of angiogenesis.
Am J Pathol. 185 (8): 2324-35.
Toffoli, B. et al. (2017) Nephropathy in Pparg-null mice highlights PPARγ systemic activities in metabolism and in the immune system.
PLoS One. 12 (2): e0171474.
Yanagida, K. et al. (2017) Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1.
Proc Natl Acad Sci U S A. 114 (17): 4531-6.
Fernández-robredo, P. et al. (2017) Neuropilin 1 Involvement in Choroidal and Retinal Neovascularisation.
PLoS One. 12 (1): e0169865.
Kim, B. et al. (2018) Endothelial pyruvate kinase M2 maintains vascular integrity.
J Clin Invest. 128 (10): 4543-56.
Villard, O. et al. (2020) Characterization of immortalized human islet stromal cells reveals a MSC-like profile with pancreatic features.
Stem Cell Res Ther. 11 (1): 158.
Chu, C.J. et al. (2013) Assessment and in vivo scoring of murine experimental autoimmune uveoretinitis using optical coherence tomography.
PLoS One. 8 (5): e63002.
Krivanek, J. et al. (2020) Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 11 (1): 4816.
Elabi, O. et al. (2021) Human α-synuclein overexpression in a mouse model of Parkinson's disease leads to vascular pathology, blood brain barrier leakage and pericyte activation.
Sci Rep. 11 (1): 1120.
Månberg, A. et al. (2021) Altered perivascular fibroblast activity precedes ALS disease onset.
Nat Med. 27 (4): 640-6.
Stone, O.A. et al. (2018) Loss of pyruvate kinase M2 limits growth and triggers innate immune signaling in endothelial cells.
Nat Commun. 9 (1): 4077.

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