Collagen IV antibody




























Filter by Application:
IF P WB C ResetRabbit anti Mouse Collagen IV
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Collagen IV
Rabbit anti Mouse Collagen IV antibody recognizes mouse collagen type IV. Collagen IV is a 1682 amino acid ~160 kDa (predicted) matrx protein and major component of glomerular basement membranes. Multiple isoforms exist each capable of forming triple helical structures with two other chains to form the type IV collagen network. The collagen IV alpha chain can be cleaved between residues 1444-1445 to yield the c-terminal 225 amino acid, ~28 kDa arresten fragment, collagen α2(IV) yields a c-terminal canstatin fragment while Collagen α3(IV) yeilds a tumstatin fragment. Collagen IV bears a single collagen IV NC1 (C-terminal non-collagenous) domain (UniProt: Q9QZR9). Mutations in collagen IV genes have been implicated in inherited nephropathies and potentially in cystic kidney disease and intracranial aneurysms (Plaisier et al. 2007). Rabbit amti Mouse Collagen IV antibody has been successfully employed for the detection of collagen IV by immunofluorescence and immunohistochemistry in mice (Tang et al. 2010), rats (Shen et al. 2014) and oragutan (Bredies et al. 2013). The following cross reactivities have been observed:
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- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Orangutan Rat Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified Ig - liquid
- Preparation
- Purified IgG prepared by antigen column chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
Antibiotic antimycotic mixture 1% - Immunogen
- Collagen IV purified from mouse EHS tumor.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
1/2000 | |
Immunofluorescence | ![]() |
1/40 | |
Immunohistology - Frozen | ![]() |
1/500 | |
Immunohistology - Paraffin | ![]() |
1/500 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Description | Sheep anti Rabbit IgG:FITC | ||||||
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Description | Sheep anti Rabbit IgG:RPE |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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Description | Antigen Retrieval Buffer, pH8.0 |
References for Collagen IV antibody
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Kojima, T. et al. (2007) Proangiogenic role of ephrinB1/EphB1 in basic fibroblast growth factor-induced corneal angiogenesis.
Am J Pathol. 170: 764-73. -
Fantin, A. et al. (2010) Tissue macrophages act as cellular chaperones for vascular anastomosis downstream of VEGF-mediated endothelial tip cell induction.
Blood.116: 829-40. -
Chen, M. et al. (2010) Immune activation in retinal aging: a gene expression study.
Invest Ophthalmol Vis Sci. 51: 5888-96. -
Rubin, A.N. et al. (2010) The germinal zones of the basal ganglia but not the septum generate GABAergic interneurons for the cortex.
J Neurosci. 30: 12050-62. -
Scott, A. et al. (2010) Astrocyte-derived vascular endothelial growth factor stabilizes vessels in the developing retinal vasculature.
PLoS One. 5: e11863. -
Armulik, A. et al. (2010) Pericytes regulate the blood-brain barrier.
Nature. 468: 557-61. -
Tang, Z. et al. (2010) Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
J Exp Med. 207: 867-80.
View The Latest Product References
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Zuercher, J. et al. (2012) Norrin stimulates cell proliferation in the superficial retinal vascular plexus and is pivotal for the recruitment of mural cells.
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Arnold, T.D. et al. (2012) Defective retinal vascular endothelial cell development as a consequence of impaired integrin αVβ8-mediated activation of transforming growth factor-β.
J Neurosci. 32: 1197-206. -
Takagi, N. et al. (2012) Mineralocorticoid Receptor Blocker Protects against Podocyte-Dependent Glomerulosclerosis.
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Ma, S. et al. (2012) Ric-8a, a Guanine Nucleotide Exchange Factor for Heterotrimeric G Proteins, Regulates Bergmann Glia-Basement Membrane Adhesion during Cerebellar Foliation.
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Edgar, K. et al. (2012) eNOS Overexpression Exacerbates Vascular Closure in the Obliterative Phase of OIR and Increases Angiogenic Drive in the Subsequent Proliferative Stage.
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Bredies, K. et al. (2013) Computer-assisted counting of retinal cells by automatic segmentation after TV denoising.
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Shen, W. et al. (2014) Systemic Administration of Erythropoietin Inhibits Retinopathy in RCS Rats.
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Williams, J.A. et al. (2016) Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina.
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Gurnik, S. et al. (2016) Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling.
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Mohanta, S.K. et al. (2022) Neuroimmune cardiovascular interfaces control atherosclerosis.
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- RRID
- AB_2082660
- UniProt
- P02463
- P08122
- Q9QZS0
- Q9QZR9
- Q80V57
- Q6PFB1
- Entrez Gene
- Col4a1
- Col4a2
- Col4a4
- Col4a3
- GO Terms
- GO:0001525 angiogenesis
- GO:0005201 extracellular matrix structural constituent
- GO:0005587 collagen type IV
- GO:0007528 neuromuscular junction development
- GO:0005488 binding
- GO:0032836 glomerular basement membrane development
- GO:0007155 cell adhesion
- GO:0005178 integrin binding
- GO:0006917 induction of apoptosis
- View More GO Terms
- GO:0006919 activation of caspase activity
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0008283 cell proliferation
- GO:0016525 negative regulation of angiogenesis
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