Collagen IV antibody
Rabbit anti Mouse collagen 4 antibody (2150-1470) used for detectiuon of collagen 4 in rat retina by immunofluorescence on cryostat sections.
Image caption:
Retinal gliosis and subretinal neovascularization in RCS rats. Double label immunostaining was performed using antibodies against vimentin (red) and collagen type IV (green) on frozen sections. (A) Non-dystrophic rat at 21 weeks (w) of age showed a normal distribution of retinal vessels confined to the inner retina and absence of glial cells in the subretinal space. (B, C) RCS rats at 6 w (B) and 9 w (C) of age showed activation of Muller cells but without subretinal neovascularization. Muller cell invasion into the subretinal space was more obvious at 9 w compared with 6 w of age (arrows in C). (D) Subretinal neovascularization (arrows) in RCS rats at 13 w of age. GCL = ganglion cell layer, INL = inner nuclear layer, OPL = outer plexiform layer, ONL = outer nuclear layer, SP = subretinal space, CC = choroidal capillaries. Scale bars: 50 μm.
From: Shen W, Chung SH, Irhimeh MR, Li S, Lee S-R, et al. (2014)
Systemic Administration of Erythropoietin Inhibits Retinopathy in RCS Rats.
PLoS ONE 9(8): e104759.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
Smooth muscle actin and CollagenIV levels are not altered in Elk3(−/−) knockout retinae. (A) IsolectinB4 (ILB4) (left) and smooth muscle actin (SMA) (middle and right) staining of retinal flat-mounts of control (upper panel) and Elk3(−/−) knockout animals. (B) IsolectinB4 (left) and collagenIV (middle and right) staining of retinal flat-mounts of control (upper panel) and Elk3(−/−) knockout animals. (C) Quantitation of collagenIV RNA levels in control and Elk3(−/−) knockout retinae (n = 5 independent experiments). (D) Representative pairs of control and Elk3(−/−) knockout retinal lysates tested for smooth muscle actin expression in Western Blot analysis. (E) Quantitation of control and Elk3(−/−) knockout retinal levels of SMA (n = 5 independent experiments). Scale bar in (A, B left and middle) 1000 μm, in (A, B right) 100 μm.
From: Weinl C, Wasylyk C, Garcia Garrido M, Sothilingam V, Beck SC, et al. (2014)
Elk3 Deficiency Causes Transient Impairment in Post-Natal Retinal Vascular Development and Formation of Tortuous Arteries in Adult Murine Retinae.
PLoS ONE 9(9): e107048.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
Vhltrp1-creKO mice demonstrate a Hif1a-dependent persistence of the pupillary membrane. (A-I) Autofluorescence scanning laser ophthalmoscope (AF-SLO) imaging (A,D,G), indocyanin-green angiography (ICGA; B,E,H) and collagen IV-stained cryosections (C,F,I) of Vhltrp1-creKO, Vhl;Hif1atrp1-creKO and control littermates at P21 show a persistent pupillary membrane in Vhltrp1-creKO mice that is absent in Vhl;Hif1atrp1-creKO and control mice. CB, ciliary body. Scale bar: 75 μm.
From: Lange CA, Luhmann UF, Mowat FM, Georgiadis A, West EL, Abrahams S, Sayed H, Powner MB, Fruttiger M, Smith AJ, Sowden JC, Maxwell PH, Ali RR, Bainbridge JW.
Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.
Development. 2012 Jul;139(13):2340-50.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Mouse collagen IV antibody (2150-1470) used for the evaluation of collagen IV expression in retinal flat-mounts by immunofluorescence.
Image caption:
Vhltrp1-creKO mice show a persistence of the hyaloid vasculature and a disorganised retinal vasculature that is independent of Hif1a. (A,B,F,G,K,L) Indocyanin-green angiography (A,F,K) and corresponding collagen 4-stained retinal flatmounts (B,G,L) show a persistent hyaloid vasculature in Vhltrp1-creKO and Vhl;Hif1atrp1-creKO mice that is absent in control littermates at P21. Inset in K shows angiography focussed on the persistent hyaloid vasculature. Inset in L shows a light microscopy image of a retinal flat mount with haemorrhage. (C,H,M) Vhltrp1-creKO and Vhl;Hif1atrp1-creKO mice demonstrate a disorganised retinal vasculature with areas of intra-retinal vascular proliferation compared with age-matched controls (hyaloid vessels were removed). Outlined regions indicate the areas selected for IMARES imaging. (D,E,I,J,N,O) IMARES reconstruction reveals a disorganised retinal vasculature with abrogated vascular layering and retinal vessels growing into the outer retina in Vhltrp1-creKO and Vhl;Hif1atrp1-creKO mice. Scale bars: 1 mm for B; 500 μm for C,M; 700 μm for H; 50 μm for D,E,I,J,N,O.
From: Lange CA, Luhmann UF, Mowat FM, Georgiadis A, West EL, Abrahams S, Sayed H, Powner MB, Fruttiger M, Smith AJ, Sowden JC, Maxwell PH, Ali RR, Bainbridge JW.
Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.
Development. 2012 Jul;139(13):2340-50.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Mouse collagen IV antibody (2150-1470) used for the detection of collagen IV in mouse retina by immunofluorescence.
Image caption:
Deletion of Vhl in the RPE results in disruption of the retinal vascular architecture and formation of chorioretinal anastomoses. (A-L) Representative collagen IV-stained cryosections of control (A-D), Vhltrp1-creKO mice (E-H) and Vhl;Hif1atrp1-creKO (I-L) and at different time points during development show a disorganised retinal vasculature and the formation of chorioretinal anastomoses in Vhl;Hif1atrp1-creKO and Vhltrp1-creKO mice (G,K). GCL, ganglion cell layer; NBL, neuroblast layer; Cho, choroid; INL, inner nuclear layer, ONL, outer nuclear layer. Scale bars: 25 μm.
From: Lange CA, Luhmann UF, Mowat FM, Georgiadis A, West EL, Abrahams S, Sayed H, Powner MB, Fruttiger M, Smith AJ, Sowden JC, Maxwell PH, Ali RR, Bainbridge JW.
Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.
Development. 2012 Jul;139(13):2340-50.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Mouse collagen IV antibody (2150-1470) used to visualize collagen IV expression in murine retina by immunofluorecence.
Image caption:
Overexpression of ATX causes abnormal vessel morphology and vessel regression. (A and B) Magnification view of angiogenic front in retina from ATX cTg mice at P6. Control and ATX cTg retinas had similar filopodia protrusion. Scale bar, 50 μm. TM, tamoxifen. (C and D) ATX cTg retinas displayed vessel regression at vascular plexus. Control (wild type) and ATX cTg retinas labeled for CD31 (green) and collagen IV (red). Arrows highlight empty collagen IV sleeves, indicating vessel regression. Scale bar, 100 μm. (E and F) Vessel regression was evaluated quantitatively. Error bars indicate s.d. (control; n = 7, ATX cTg; n = 5). P-values were estimated by student’s t-test, ***P < 0.001. Data were pooled from three independent experiments.
From: Yukiura H, Kano K, Kise R, Inoue A, Aoki J (2015)
Autotaxin Overexpression Causes Embryonic Lethality and Vascular Defects.Citation: Yukiura H, Kano K, Kise R, Inoue A, Aoki J (2015)
Autotaxin Overexpression Causes Embryonic Lethality and Vascular Defects.
PLoS ONE 10(5): e0126734.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Mouse collagen IV antibody (2150-1470) used for the detection of collagen IV in Murine choroidal flatmounts by immunofluorescence on paraformaldehyde fixed sections.
Image caption:
Choroidal neovascularisation in CCL2−/−CX3CR1GFP/GFP DKO mice. (A) CNV was induced in adult (8∼12-week) WT and CCL2−/−CX3CR1GFP/GFP mice. RPE/choroidal flatmounts were collected at day 10 and stained for isolectin B4 (green) and collagen IV (red) and examined by confocal microscopy. (B) CNV size in WT and CCL2−/−CX3CR1GFP/GFP mice at day 10. N = 6. (C, D) VEGF-A mRNA expression in RPE cells (C) and BM-DMs (D) from WT and CCL2−/−CX3CR1GFP/GFP mice. N = 5.
From: Chen M, Hombrebueno JR, Luo C, Penalva R, Zhao J, Colhoun L, et al. (2013)
Age- and Light-Dependent Development of Localised Retinal Atrophy in CCL2−/−CX3CR1GFP/GFP Mice.
PLoS ONE 8(4): e61381.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Murine cytokeratin 4 antibody (2150-1470) used for the identification of collagen IV expressing vasculature in mouse brains by immunofluorescence on vibratome sections.
Image caption:
Immunohistochemistry analysis of pericytes in Ang-2 GOF mice. a Pericytes (desmin+) were decreased in GOF mice utilizing 10 μm cryosections for analysis (n = 8, scale bars 25μm). b 50 μm vibratome sections of Ang-2 GOF and WT mice revealed decrease in pericyte area/vessel area [%] (n = 3, scale bars 10 μm) indicating decreased pericyte coverage of the vessels in GOF mice
From: Gurnik S, Devraj K, Macas J, Yamaji M, Starke J, Scholz A, Sommer K, Di Tacchio M, Vutukuri R, Beck H, Mittelbronn M, Foerch C, Pfeilschifter W, Liebner S, Peters KG, Plate KH, Reiss Y.
Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling.
Acta Neuropathol. 2016 May;131(5):753-73.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Human collagen IV antibody (2150-1470) showing increased renal collagen IV deposition in aged PpargΔ/Δ mice over controls by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemistry and gene expression of fibrosis markers in kidney of ageing PpargΔ/Δ mice, and plasmatic levels of anti-β2-glycoprotein 1 antibodies.
52 weeks old control (CTL) and PparΔ/Δ (γΔ/Δ) animals were analyzed for the following parameters: (A) Top panels: Kidney Immunohistochemistry for collagen IV (Col-IV). Positive staining is in brown. Scale bar represents 100μm.
From: Toffoli B, Gilardi F, Winkler C, Soderberg M, Kowalczuk L, Arsenijevic Y, et al. (2017)
Nephropathy in Pparg-null mice highlights PPARγ systemic activities in metabolism and in the immune system.
PLoS ONE 12(2): e0171474.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Human collagen IV antibody (2150-4140) used for the evaluation of collagen IV expression in human stem cell preparations by western blotting.
Image caption:
hISCs secrete pancreatic ECM proteins. Extracted proteins from hISCs and BM-MSCs and corresponding conditioned media were analyzed by western blotting. Type I, IV, and VI collagens as well as fibronectin and laminin were detected in both cell types but at different levels of expression. β-actin was used as loading control. The right panels represent the quantification of the Western blot, n = 4, *p <0.05, significant differences
From: Villard O, Armanet M, Couderc G, et al.
Characterization of immortalized human islet stromal cells reveals a MSC-like profile with pancreatic features.
Stem Cell Res Ther. 2020;11(1):158. doi:10.1186/s13287-020-01649-z
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Mouse Collagen IV antibody recognizes mouse collagen type IV. Collagen IV is a 1682 amino acid ~160 kDa (predicted) matrx protein and major component of glomerular basement membranes. Multiple isoforms exist each capable of forming triple helical structures with two other chains to form the type IV collagen network. The collagen IV alpha chain can be cleaved between residues 1444-1445 to yield the c-terminal 225 amino acid, ~28 kDa arresten fragment, collagen α2(IV) yields a c-terminal canstatin fragment while Collagen α3(IV) yeilds a tumstatin fragment. Collagen IV bears a single collagen IV NC1 (C-terminal non-collagenous) domain (UniProt: Q9QZR9).
Mutations in collagen IV genes have been implicated in inherited nephropathies and potentially in cystic kidney disease and intracranial aneurysms (Plaisier et al. 2007).
Rabbit amti Mouse Collagen IV antibody has been successfully employed for the detection of collagen IV by immunofluorescence and immunohistochemistry in mice (Tang et al. 2010), rats (Shen et al. 2014) and oragutan (Bredies et al. 2013).
The following cross reactivities have been observed:
Mutations in collagen IV genes have been implicated in inherited nephropathies and potentially in cystic kidney disease and intracranial aneurysms (Plaisier et al. 2007).
Rabbit amti Mouse Collagen IV antibody has been successfully employed for the detection of collagen IV by immunofluorescence and immunohistochemistry in mice (Tang et al. 2010), rats (Shen et al. 2014) and oragutan (Bredies et al. 2013).
The following cross reactivities have been observed:
| Mouse type IV | 100% |
| Mouse types I, II & III | <0.1% |
| Human types IV & V | <0.1% |
| Mouse fibronectin & laminin | <0.1% |
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Orangutan Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified Ig - liquid
- Preparation
- Purified IgG prepared by antigen column chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
Antibiotic antimycotic mixture 1%0.09% Sodium Azide (NaN3) - Immunogen
- Collagen IV purified from mouse EHS tumor.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P02463
- P08122
- Q9QZS0
- Q9QZR9
- Q80V57
- Q6PFB1
- Entrez Gene
- Col4a1
- Col4a2
- Col4a4
- Col4a3
- GO Terms
- GO:0001525 angiogenesis
- GO:0005201 extracellular matrix structural constituent
- GO:0005587 collagen type IV
- GO:0007528 neuromuscular junction development
- GO:0005488 binding
- GO:0032836 glomerular basement membrane development
- GO:0007155 cell adhesion
- GO:0005178 integrin binding
- GO:0006917 induction of apoptosis
- GO:0006919 activation of caspase activity
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0008283 cell proliferation
- GO:0016525 negative regulation of angiogenesis
- Regulatory
- For research purposes only
Applications of Collagen IV antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | ||
| Immunofluorescence | 1/40 | ||
| Immunohistology - Frozen | 1/500 | ||
| Immunohistology - Paraffin | 1/500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for Collagen IV antibody
-
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J Exp Med. 207: 867-80. -
Fantin, A. et al. (2010) Tissue macrophages act as cellular chaperones for vascular anastomosis downstream of VEGF-mediated endothelial tip cell induction.
Blood.116: 829-40. -
Shen, W. et al. (2014) Systemic Administration of Erythropoietin Inhibits Retinopathy in RCS Rats.
PLoS One. 9: e104759. -
Bredies, K. et al. (2013) Computer-assisted counting of retinal cells by automatic segmentation after TV denoising.
BMC Ophthalmol. 13: 59. -
Rubin, A.N. et al. (2010) The germinal zones of the basal ganglia but not the septum generate GABAergic interneurons for the cortex.
J Neurosci. 30: 12050-62. -
Weinl, C. et al. (2014) Elk3 deficiency causes transient impairment in post-natal retinal vascular development and formation of tortuous arteries in adult murine retinae.
PLoS One. 9: e107048. -
Stenzel, D. et al. (2011) Integrin-dependent and -independent functions of astrocytic fibronectin in retinal angiogenesis.
Development. 138: 4451-63. -
Zuercher, J. et al. (2012) Norrin stimulates cell proliferation in the superficial retinal vascular plexus and is pivotal for the recruitment of mural cells.
Hum Mol Genet. 21: 2619-30. -
Arnold, T.D. et al. (2012) Defective retinal vascular endothelial cell development as a consequence of impaired integrin αVβ8-mediated activation of transforming growth factor-β.
J Neurosci. 32: 1197-206. -
Chen, M. et al. (2010) Immune activation in retinal aging: a gene expression study.
Invest Ophthalmol Vis Sci. 51: 5888-96. -
Kojima, T. et al. (2007) Proangiogenic role of ephrinB1/EphB1 in basic fibroblast growth factor-induced corneal angiogenesis.
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Ma, S. et al. (2012) Ric-8a, a Guanine Nucleotide Exchange Factor for Heterotrimeric G Proteins, Regulates Bergmann Glia-Basement Membrane Adhesion during Cerebellar Foliation.
J Neurosci. 32: 14979-93. -
Dulauroy, S. et al. (2012) Lineage tracing and genetic ablation of ADAM12(+) perivascular cells identify a major source of profibrotic cells during acute tissue injury.
Nat Med. 18: 1262-70. -
Edgar, K. et al. (2012) eNOS Overexpression Exacerbates Vascular Closure in the Obliterative Phase of OIR and Increases Angiogenic Drive in the Subsequent Proliferative Stage.
Invest Ophthalmol Vis Sci. 53: 6833-50. -
Lange, C.A. et al. (2012) Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.
Development. 139: 2340-50. -
Li, W. and Mukouyama, Y.S. (2011) Whole-mount immunohistochemical analysis for embryonic limb skin vasculature: a model system to study vascular branching morphogenesis in embryo.
J Vis Exp. 51: pii: 2620. -
Lutter, S. et al. (2012) Smooth muscle-endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation.
J Cell Biol. 197: 837-49. -
McKenzie, J.A. et al. (2012) Apelin is required for non-neovascular remodeling in the retina.
Am J Pathol. 180: 399-409. -
Powner, M.B. et al. (2012) Visualization of gene expression in whole mouse retina by in situ hybridization.
Nat Protoc. 7: 1086-96. -
Schulz, C. et al. (2012) A lineage of myeloid cells independent of Myb and hematopoietic stem cells.
Science. 336: 86-90. -
Scott, A. et al. (2010) Astrocyte-derived vascular endothelial growth factor stabilizes vessels in the developing retinal vasculature.
PLoS One. 5: e11863. -
Takagi, N. et al. (2012) Mineralocorticoid Receptor Blocker Protects against Podocyte-Dependent Glomerulosclerosis.
Nephron Extra. 2: 17-26. -
Chen, M. et al. (2013) Age- and light-dependent development of localised retinal atrophy in CCL2(-/-)CX3CR1(GFP/GFP) mice.
PLoS One. 8: e61381. -
Luhmann UF. et al. (2005) Berger W. Role of the Norrie disease pseudoglioma gene in sprouting angiogenesis
during development of the retinal vasculature.
Invest Ophthalmol Vis Sci. 46: 3372-82. -
Scott, A. et al. (2014) Quantification of vascular tortuosity as an early outcome measure in oxygen induced retinopathy (OIR)
Exp Eye Res. 120: 55-60. -
Yukiura H et al. (2015) Autotaxin overexpression causes embryonic lethality and vascular defects.
PLoS One. 10 (5): e0126734. -
Williams, J.A. et al. (2016) Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina.
PLoS One. 11 (8): e0161898. -
Piñero G et al. (2016) Lithium Reversibly Inhibits Schwann Cell Proliferation and Differentiation Without Inducing Myelin Loss.
Mol Neurobiol. Dec 5. [Epub ahead of print] -
Gurnik, S. et al. (2016) Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling.
Acta Neuropathol. 131 (5): 753-73. -
Misra, A. et al. (2016) Integrin β3 inhibition is a therapeutic strategy for supravalvular aortic stenosis.
J Exp Med. 213 (3): 451-63. -
Wu, W.K. et al. (2015) IL-4 regulates specific Arg-1(+) macrophage sFlt-1-mediated inhibition of angiogenesis.
Am J Pathol. 185 (8): 2324-35. -
Toffoli, B. et al. (2017) Nephropathy in Pparg-null mice highlights PPARγ systemic activities in metabolism and in the immune system.
PLoS One. 12 (2): e0171474. -
Yanagida, K. et al. (2017) Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1.
Proc Natl Acad Sci U S A. 114 (17): 4531-6. -
Fernández-robredo, P. et al. (2017) Neuropilin 1 Involvement in Choroidal and Retinal Neovascularisation.
PLoS One. 12 (1): e0169865. -
Kim, B. et al. (2018) Endothelial pyruvate kinase M2 maintains vascular integrity.
J Clin Invest. 128 (10): 4543-56. -
Villard, O. et al. (2020) Characterization of immortalized human islet stromal cells reveals a MSC-like profile with pancreatic features.
Stem Cell Res Ther. 11 (1): 158.
