Collagen I antibody
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Rabbit anti Mouse Collagen I antibody recognizes murine collagen 1. Collagen 1 is a fibrillar forming collagen, a secreted protein forming part of the extracellular matrix. Less than 1% cross reactivity has been observed with mouse collagen, types II, VI and III and human, chicken and rat collagen, type I.
Product Details
- Target Species
- Mouse
- Product Form
- Purified Ig - liquid
- Preparation
- Purified Ig prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
Antibiotic antimycotic mixture 1% - Immunogen
- Native type I collagen from mouse skin.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P11087
- Q01149
- Entrez Gene
- Col1a1
- Col1a2
- GO Terms
- GO:0001568 blood vessel development
- GO:0001649 osteoblast differentiation
- GO:0001957 intramembranous ossification
- GO:0001958 endochondral ossification
- GO:0005201 extracellular matrix structural constituent
- GO:0005584 collagen type I
- GO:0005737 cytoplasm
- GO:0010812 negative regulation of cell-substrate adhesion
- GO:0015031 protein transport
- GO:0060325 face morphogenesis
- GO:0060346 bone trabecula formation
- GO:0060351 cartilage development involved in endochondral bone morphogenesis
- GO:0070208 protein heterotrimerization
- GO:0046332 SMAD binding
- Regulatory
- For research purposes only
Applications of Collagen I antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100 | 1/400 | |
| Immunofluorescence | 1/50 | 1/100 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | 1/250 | 1/1000 | |
| Radioimmunoassays | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for Collagen I antibody
-
Uezumi, A. et al. (2011) Fibrosis and adipogenesis originate from a common mesenchymal progenitor in skeletal muscle.
J Cell Sci. 124: 3654-64. -
Takase, H.M. et al. (2013) FGF7 is a functional niche signal required for stimulation of adult liver progenitor cells that support liver regeneration.
Genes Dev. 27: 169-81. -
Birbrair A et al. (2013) Type-1 pericytes participate in fibrous tissue deposition in aged skeletal muscle.
Am J Physiol Cell Physiol. 305 (11): C1098-113. -
Randles, M.J. et al. (2015) Genetic Background is a Key Determinant of Glomerular Extracellular Matrix Composition and Organization.
J Am Soc Nephrol. 26 (12): 3021-34. -
Ma, M.S. et al. (2016) Characterization and comparison of osteoblasts derived from mouse embryonic stem cells and induced pluripotent stem cells.
J Bone Miner Metab. Jan 8. [Epub ahead of print] -
Valero-Muñoz, V. et al. (2019) Supplementation with an insoluble fiber obtained from carob pod (Ceratonia siliqua L.) rich in polyphenols prevents dyslipidemia in rabbits through SIRT1/PGC-1α pathway.
Eur J Nutr. 58 (1): 357-66.
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