Collagen I antibody

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Rabbit anti Mouse Collagen I

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
2150-1410 Datasheet	C E IF P R WB SDS	0.1 ml	Log in		Get Quote

Rabbit anti Mouse Collagen I antibody recognizes murine collagen 1. Collagen 1 is a fibrillar forming collagen, a secreted protein forming part of the extracellular matrix. Less than 1% cross reactivity has been observed with mouse collagen, types II, VI and III and human, chicken and rat collagen, type I.

Product Details

Target Species: Mouse
Product Form: Purified Ig - liquid
Preparation: Purified Ig prepared by affinity chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Antibiotic antimycotic mixture 1%
Immunogen: Native type I collagen from mouse skin.

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P11087; Q01149
Entrez Gene: Col1a1; Col1a2
GO Terms: GO:0001568 blood vessel development; GO:0001649 osteoblast differentiation; GO:0001957 intramembranous ossification; GO:0001958 endochondral ossification; GO:0005201 extracellular matrix structural constituent; GO:0005584 collagen type I; GO:0005737 cytoplasm; GO:0010812 negative regulation of cell-substrate adhesion; GO:0015031 protein transport; GO:0060325 face morphogenesis; GO:0060346 bone trabecula formation; GO:0060351 cartilage development involved in endochondral bone morphogenesis; GO:0070208 protein heterotrimerization; GO:0046332 SMAD binding
Regulatory: For research purposes only

Applications of Collagen I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/100	1/400
Immunofluorescence	1/50	1/100
Immunohistology - Frozen
Immunohistology - Paraffin	1/250	1/1000
Radioimmunoassays
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in		Get Quote
100x Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in		Get Quote

Application Based External Images

Western Blotting

Product Specific References

References for Collagen I antibody

Uezumi, A. et al. (2011) Fibrosis and adipogenesis originate from a common mesenchymal progenitor in skeletal muscle.
J Cell Sci. 124: 3654-64.
Takase, H.M. et al. (2013) FGF7 is a functional niche signal required for stimulation of adult liver progenitor cells that support liver regeneration.
Genes Dev. 27: 169-81.
Birbrair A et al. (2013) Type-1 pericytes participate in fibrous tissue deposition in aged skeletal muscle.
Am J Physiol Cell Physiol. 305 (11): C1098-113.
Randles, M.J. et al. (2015) Genetic Background is a Key Determinant of Glomerular Extracellular Matrix Composition and Organization.
J Am Soc Nephrol. 26 (12): 3021-34.
Ma, M.S. et al. (2016) Characterization and comparison of osteoblasts derived from mouse embryonic stem cells and induced pluripotent stem cells.
J Bone Miner Metab. Jan 8. [Epub ahead of print]
Valero-Muñoz, V. et al. (2019) Supplementation with an insoluble fiber obtained from carob pod (Ceratonia siliqua L.) rich in polyphenols prevents dyslipidemia in rabbits through SIRT1/PGC-1α pathway.
Eur J Nutr. 58 (1): 357-66.

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