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Collagen I antibody

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Rabbit anti Mouse Collagen I

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
2150-1410
Datasheet
C E IF P R WB
MSDS
0.1 ml loader

Rabbit anti Mouse Collagen I antibody recognizes murine collagen 1. Collagen 1 is a fibrillar forming collagen, a secreted protein forming part of the extracellular matrix. Less than 1% cross reactivity has been observed with mouse collagen, types II, VI and III and human, chicken and rat collagen, type I.

Product Details

Target Species
Mouse
Product Form
Purified Ig - liquid
Preparation
Purified Ig prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Antibiotic antimycotic mixture 1%
Immunogen
Native type I collagen from mouse skin.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P11087
Q01149
Entrez Gene
Col1a1
Col1a2
GO Terms
GO:0001568 blood vessel development
GO:0001649 osteoblast differentiation
GO:0001957 intramembranous ossification
GO:0001958 endochondral ossification
GO:0005201 extracellular matrix structural constituent
GO:0005584 collagen type I
GO:0005737 cytoplasm
GO:0010812 negative regulation of cell-substrate adhesion
GO:0015031 protein transport
GO:0060325 face morphogenesis
GO:0060346 bone trabecula formation
GO:0060351 cartilage development involved in endochondral bone morphogenesis
GO:0070208 protein heterotrimerization
GO:0046332 SMAD binding
Regulatory
For research purposes only

Applications of Collagen I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/400
Immunofluorescence 1/50 1/100
Immunohistology - Frozen
Immunohistology - Paraffin 1/250 1/1000
Radioimmunoassays
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Application Based External Images

Western Blotting

Product Specific References

References for Collagen I antibody

  1. Uezumi, A. et al. (2011) Fibrosis and adipogenesis originate from a common mesenchymal progenitor in skeletal muscle.
    J Cell Sci. 124: 3654-64.
  2. Takase, H.M. et al. (2013) FGF7 is a functional niche signal required for stimulation of adult liver progenitor cells that support liver regeneration.
    Genes Dev. 27: 169-81.
  3. Birbrair A et al. (2013) Type-1 pericytes participate in fibrous tissue deposition in aged skeletal muscle.
    Am J Physiol Cell Physiol. 305 (11): C1098-113.
  4. Randles, M.J. et al. (2015) Genetic Background is a Key Determinant of Glomerular Extracellular Matrix Composition and Organization.
    J Am Soc Nephrol. 26 (12): 3021-34.
  5. Ma, M.S. et al. (2016) Characterization and comparison of osteoblasts derived from mouse embryonic stem cells and induced pluripotent stem cells.
    J Bone Miner Metab. Jan 8. [Epub ahead of print]
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