ATG5-ATG12 Complex antibody
Rabbit anti ATG5-ATG12 Complex antibody (AAM79) used for the evaluation of ATG5-ATG12 conjugate expression in OVCAR-3 cells following induction of autophagy by western blotting.
Image caption:
The time course of autophagy and apoptosis induction by 30 μM resveratrol treatment. A. Live cell imaging by confocal microscopy (with 10X objective) with Cyto-ID dye in OVCAR-3 cells treated with 30 μM resveratrol at 0, 12, 24 and 48 hour time points. B. Live cell imaging by confocal microscopy (with 10X objective) with caspase 3 green detection dye in OVCAR-3 cells at 0, 12, 24 and 48 hour time points. C. OVCAR-3 cells were treated with 30 μM of resveratrol for 12 h, 24 h, and 48 h. Western blotting of total cell lysates were performed with anti-ATG5-ATG12, LC3, and caspase 3 antibodies. No cleavage of ATG5 was observed. The results shown represent an experiment, in which each time-point of treatment was duplicated.
From: Lang F, Qin Z, Li F, Zhang H, Fang Z, Hao E (2015) Apoptotic Cell Death Induced by Resveratrol Is Partially Mediated by the Autophagy Pathway in Human Ovarian Cancer Cells.
PLoS ONE 10(6): e0129196.
Rabbit anti ATG5-ATG12 Complex antibody recognizes an epitope within the C-Terminal (CT) region of the Atg5-Atg12 complex, which plays a key role in the regulation of autophagy, and is emerging as an important factor in the innate immune response to viruses (Hwang et al. 2012).
The Atg5-Atg12 complex interacts with retinoic acid–inducible gene 1 and the adaptor molecule IFN-beta promoter stimulator 1, through caspase recruitment domains, thereby acting as a negative regulator of the type I IFN production pathway, and as a contributor to RNA viral replication within the host cells (Takeshita et al 2008).
The Atg5-Atg12 complex interacts with retinoic acid–inducible gene 1 and the adaptor molecule IFN-beta promoter stimulator 1, through caspase recruitment domains, thereby acting as a negative regulator of the type I IFN production pathway, and as a contributor to RNA viral replication within the host cells (Takeshita et al 2008).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to mouse Atg5 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide corresponding to amino acids 262-275 of mouse Atg5. The corresponding sequence differs by one amino acid in human.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- Q99J83
- Q9H1Y0
- Q9CQY1
- O94817
- Entrez Gene
- ATG12
- Atg5
- Atg12
- ATG5
- GO Terms
- GO:0000045 autophagic vacuole assembly
- GO:0005515 protein binding
- GO:0032480 negative regulation of type I interferon production
- GO:0034045 pre-autophagosomal structure membrane
- GO:0001974 blood vessel remodeling
- GO:0005829 cytosol
- GO:0005776 autophagic vacuole
- GO:0042493 response to drug
- GO:0031397 negative regulation of protein ubiquitination
- GO:0042311 vasodilation
- GO:0043066 negative regulation of apoptosis
- GO:0043687 post-translational protein modification
- GO:0048840 otolith development
- GO:0055015 ventricular cardiac muscle cell development
- GO:0060047 heart contraction
- GO:0006915 apoptosis
- Regulatory
- For research purposes only
Applications of ATG5-ATG12 Complex antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1.0 | 2.0ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AAM79 detects a band of approximately 56kDa in mouse liver and human U87 cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for ATG5-ATG12 Complex antibody
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Lang, F. et al. (2015) Apoptotic Cell Death Induced by Resveratrol Is Partially Mediated by the Autophagy Pathway in Human Ovarian Cancer Cells.
PLoS One. 10 (6): e0129196.
