ATG5-ATG12 Complex antibody
The Atg5-Atg12 complex interacts with retinoic acid–inducible gene 1 and the adaptor molecule IFN-beta promoter stimulator 1, through caspase recruitment domains, thereby acting as a negative regulator of the type I IFN production pathway, and as a contributor to RNA viral replication within the host cells (Takeshita et al 2008).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to mouse Atg5 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide corresponding to amino acids 262-275 of mouse Atg5. The corresponding sequence differs by one amino acid in human.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q99J83
- Q9H1Y0
- Q9CQY1
- O94817
- Entrez Gene
- ATG12
- Atg5
- Atg12
- ATG5
- GO Terms
- GO:0000045 autophagic vacuole assembly
- GO:0005515 protein binding
- GO:0032480 negative regulation of type I interferon production
- GO:0034045 pre-autophagosomal structure membrane
- GO:0001974 blood vessel remodeling
- GO:0005829 cytosol
- GO:0005776 autophagic vacuole
- GO:0042493 response to drug
- GO:0031397 negative regulation of protein ubiquitination
- GO:0042311 vasodilation
- GO:0043066 negative regulation of apoptosis
- GO:0043687 post-translational protein modification
- GO:0048840 otolith development
- GO:0055015 ventricular cardiac muscle cell development
- GO:0060047 heart contraction
- GO:0006915 apoptosis
- Regulatory
- For research purposes only
Applications of ATG5-ATG12 Complex antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1.0 | 2.0ug/ml |
- Western Blotting
- AAM79 detects a band of approximately 56kDa in mouse liver and human U87 cell lysates.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
![]() |
|
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
![]() |
|
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
![]() |
|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
![]() |
|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
![]() |
|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
![]() |
|
Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for ATG5-ATG12 Complex antibody
-
Lang, F. et al. (2015) Apoptotic Cell Death Induced by Resveratrol Is Partially Mediated by the Autophagy Pathway in Human Ovarian Cancer Cells.
PLoS One. 10 (6): e0129196.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra