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ATG5-ATG12 Complex antibody

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Anti ATG5-ATG12 Complex (C-Terminal) Antibody gallery image 1
Published customer image:
Rabbit anti ATG5-ATG12 Complex antibody (AAM79) used for the evaluation of ATG5-ATG12 conjugate expression in OVCAR-3 cells following induction of autophagy by western blotting.
Image caption:
The time course of autophagy and apoptosis induction by 30 μM resveratrol treatment. A. Live cell imaging by confocal microscopy (with 10X objective) with Cyto-ID dye in OVCAR-3 cells treated with 30 μM resveratrol at 0, 12, 24 and 48 hour time points. B. Live cell imaging by confocal microscopy (with 10X objective) with caspase 3 green detection dye in OVCAR-3 cells at 0, 12, 24 and 48 hour time points. C. OVCAR-3 cells were treated with 30 μM of resveratrol for 12 h, 24 h, and 48 h. Western blotting of total cell lysates were performed with anti-ATG5-ATG12, LC3, and caspase 3 antibodies. No cleavage of ATG5 was observed. The results shown represent an experiment, in which each time-point of treatment was duplicated.

From: Lang F, Qin Z, Li F, Zhang H, Fang Z, Hao E (2015)
Apoptotic Cell Death Induced by Resveratrol Is Partially Mediated by the Autophagy Pathway in Human Ovarian Cancer Cells.
PLoS ONE 10(6): e0129196.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rabbit anti ATG5-ATG12 Complex (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AAM79
Datasheet
WB
SDS
0.1 mg loader

Rabbit anti ATG5-ATG12 Complex antibody recognizes an epitope within the C-Terminal (CT) region of the Atg5-Atg12 complex, which plays a key role in the regulation of autophagy, and is emerging as an important factor in the innate immune response to viruses (Hwang et al. 2012).

The Atg5-Atg12 complex interacts with retinoic acid–inducible gene 1 and the adaptor molecule IFN-beta promoter stimulator 1, through caspase recruitment domains, thereby acting as a negative regulator of the type I IFN production pathway, and as a contributor to RNA viral replication within the host cells (Takeshita et al 2008).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to mouse Atg5 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide corresponding to amino acids 262-275 of mouse Atg5. The corresponding sequence differs by one amino acid in human.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q99J83
Q9H1Y0
Q9CQY1
O94817
Entrez Gene
ATG12
Atg5
Atg12
ATG5
GO Terms
GO:0000045 autophagic vacuole assembly
GO:0005515 protein binding
GO:0032480 negative regulation of type I interferon production
GO:0034045 pre-autophagosomal structure membrane
GO:0001974 blood vessel remodeling
GO:0005829 cytosol
GO:0005776 autophagic vacuole
GO:0042493 response to drug
GO:0031397 negative regulation of protein ubiquitination
GO:0042311 vasodilation
GO:0043066 negative regulation of apoptosis
GO:0043687 post-translational protein modification
GO:0048840 otolith development
GO:0055015 ventricular cardiac muscle cell development
GO:0060047 heart contraction
GO:0006915 apoptosis
Regulatory
For research purposes only

Applications of ATG5-ATG12 Complex antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1.0 2.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AAM79 detects a band of approximately 56kDa in mouse liver and human U87 cell lysates.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

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Description Product Code Applications Pack Size List Price Quantity
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Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for ATG5-ATG12 Complex antibody

  1. Lang, F. et al. (2015) Apoptotic Cell Death Induced by Resveratrol Is Partially Mediated by the Autophagy Pathway in Human Ovarian Cancer Cells.
    PLoS One. 10 (6): e0129196.

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