Vinculin antibody
Rabbit anti Human vinculin antibody recognizes vinculin, also known as metavinculin (MVCL).
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for VCL, but the biological validity of some variants has not been determined (provided by RefSeq, Jul 2008).
Rabbit anti Human vinculin antibody detects a band of 124 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for VCL, but the biological validity of some variants has not been determined (provided by RefSeq, Jul 2008).
Rabbit anti Human vinculin antibody detects a band of 124 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti vinculin antibody recognizes a band of approximately 124 kDa in LNCaP cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- Synthetic peptide corresponding to the middle region of human vinculin
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P18206
- Entrez Gene
- VCL
- GO Terms
- GO:0002009 morphogenesis of an epithelium
- GO:0002166 beta-dystroglycan binding
- GO:0002576 platelet degranulation
- GO:0006936 muscle contraction
- GO:0003779 actin binding
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0005198 structural molecule activity
- GO:0005925 focal adhesion
- GO:0006928 cellular component movement
- GO:0007160 cell-matrix adhesion
- GO:0008013 beta-catenin binding
- GO:0030032 lamellipodium assembly
- GO:0030168 platelet activation
- GO:0030336 negative regulation of cell migration
- GO:0034333 adherens junction assembly
- GO:0034394 protein localization at cell surface
- GO:0043034 costamere
- GO:0043297 apical junction assembly
- GO:0045294 alpha-catenin binding
- GO:0045296 cadherin binding
- GO:0090136 epithelial cell-cell adhesion
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Vinculin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
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