Vinculin antibody

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Rabbit anti Vinculin

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00888
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human vinculin antibody recognizes vinculin, also known as metavinculin (MVCL).

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for VCL, but the biological validity of some variants has not been determined (provided by RefSeq, Jul 2008).

Rabbit anti Human vinculin antibody detects a band of 124 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti vinculin antibody recognizes a band of approximately 124 kDa in LNCaP cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
Synthetic peptide corresponding to the middle region of human vinculin

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P18206
Entrez Gene
VCL
GO Terms
GO:0002009 morphogenesis of an epithelium
GO:0002166 beta-dystroglycan binding
GO:0002576 platelet degranulation
GO:0006936 muscle contraction
GO:0003779 actin binding
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005198 structural molecule activity
GO:0005925 focal adhesion
GO:0006928 cellular component movement
GO:0007160 cell-matrix adhesion
GO:0008013 beta-catenin binding
GO:0030032 lamellipodium assembly
GO:0030168 platelet activation
GO:0030336 negative regulation of cell migration
GO:0034333 adherens junction assembly
GO:0034394 protein localization at cell surface
GO:0043034 costamere
GO:0043297 apical junction assembly
GO:0045294 alpha-catenin binding
GO:0045296 cadherin binding
GO:0090136 epithelial cell-cell adhesion
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Vinculin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml