Vinculin antibody

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siRNA Validated

Anti Vinculin Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti Vinculin Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti Vinculin Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti Vinculin Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with vinculin antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to vinculin (molecular weight 124 kDa)

Anti Vinculin Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against Vinculin (1 & 2) or a scrambled siRNA (-) probed with Rabbit anti Vinculin antibody (**VPA00888**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 58 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.

Anti Vinculin Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of Viniculin from whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against Viniculin (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Rabbit anti Vinculin

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Vinculin

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VPA00888 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human vinculin antibody recognizes vinculin, also known as metavinculin (MVCL).

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for VCL, but the biological validity of some variants has not been determined (provided by RefSeq, Jul 2008).

Rabbit anti Human vinculin antibody detects a band of 124 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti vinculin antibody recognizes a band of approximately 124 kDa in LNCaP cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: Synthetic peptide corresponding to the middle region of human vinculin
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P18206
Entrez Gene: VCL
GO Terms: GO:0002009 morphogenesis of an epithelium; GO:0002166 beta-dystroglycan binding; GO:0002576 platelet degranulation; GO:0006936 muscle contraction; GO:0003779 actin binding; GO:0005576 extracellular region; GO:0005829 cytosol; GO:0005198 structural molecule activity; GO:0005925 focal adhesion; View More GO Terms; GO:0006928 cellular component movement; GO:0007160 cell-matrix adhesion; GO:0008013 beta-catenin binding; GO:0030032 lamellipodium assembly; GO:0030168 platelet activation; GO:0030336 negative regulation of cell migration; GO:0034333 adherens junction assembly; GO:0034394 protein localization at cell surface; GO:0043034 costamere; GO:0043297 apical junction assembly; GO:0045294 alpha-catenin binding; GO:0045296 cadherin binding; GO:0090136 epithelial cell-cell adhesion