Goat ati Human SNAP25 antibody
recognizes human synaptosomal-associated protein, 25kD (SNAP-25), also known as Super protein (Sup) or Synaptosomal-associated 25 kDa protein. SNAP-25 is a 206 amino acid, presynaptic plasma membrane protein containning 2 tSNARE coiled-coil
domains and is essential for Ca2+-regulated exocytosis of neurotransmitters. SNAP-25 binds to and negatively modulate Ca2+ channels, increasing the speed of neurotransmitter release (Rettiq et al. 1996
) .Goat ati Human SNAP25 antibody detects an epitope within the C-terminal (CT) of human SNAP25 (aa 193-206).
As a result of alternative splicing, SNAP-25 is expressed by neurons and neuroendocrine cells as two predominant isoforms; SNAP-25a and SNAP-25b. SNAP-25a is expressed early in development, whereas SNAP-25b expression is linked to synaptogenesis (Bark and Wilson 1994
). Goat ati human SNAP25 antibody is expected to recognise both isoforms.
SNAP-25 may be a possible therapeutic treatment target for sufferers of hyperactivity disorders (Barr et al. 2000
), whilst SNAP-25 based peptides may be a possible treatment for involuntary muscle spasms (Hodel 1998
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of SNAP-25 antibody
|Immunohistology - Paraffin 1
- 1Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. Bio-Rad recommend citrate buffer, pH6.0, for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1124 detects a band of approximately 22kDa in human and mouse brain lysates. A minimum incubation time of 1 hour is recommended with this antibody.