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SNAP-25 antibody

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Anti SNAP-25 (C-Terminal) Antibody thumbnail image 1 Anti SNAP-25 (C-Terminal) Antibody thumbnail image 2
Anti SNAP-25 (C-Terminal) Antibody gallery image 1
Western blotting analysis of human cerebellum) (A) and mouse brain (B) lysates (35μg protein in RIPA buffer) probed using Goat anti Human SNAP25 antibody (AHP1124) at 0.03μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Anti SNAP-25 (C-Terminal) Antibody gallery image 2
Immunohistochemical staining of formalin fixed, paraffin embedded Human Cerebellum following heat mediated antigen retrieval in citrate buffer at pH6.0. A. Cerebellum stained using Goat anti Human SNAP25 antibody (AHP1124) at 1.0μgml-1. B. Negative control showing cerebellum staining with primary antibody omitted

Goat anti SNAP-25 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1124
Datasheet
E P* WB
SDS
0.1 mg

Goat ati Human SNAP25 antibody recognizes human synaptosomal-associated protein, 25kD (SNAP-25), also known as Super protein (Sup) or Synaptosomal-associated 25 kDa protein. SNAP-25 is a 206 amino acid, presynaptic plasma membrane protein containning 2 tSNARE coiled-coil domains and is essential for Ca2+-regulated exocytosis of neurotransmitters. SNAP-25 binds to and negatively modulate Ca2+ channels, increasing the speed of neurotransmitter release (Rettiq et al. 1996) .Goat ati Human SNAP25 antibody detects an epitope within the C-terminal (CT) of human SNAP25 (aa 193-206).

As a result of alternative splicing, SNAP-25 is expressed by neurons and neuroendocrine cells as two predominant isoforms; SNAP-25a and SNAP-25b. SNAP-25a is expressed early in development, whereas SNAP-25b expression is linked to synaptogenesis (Bark and Wilson 1994). Goat ati human SNAP25 antibody is expected to recognise both isoforms.

SNAP-25 may be a possible therapeutic treatment target for sufferers of hyperactivity disorders (Barr et al. 2000), whilst SNAP-25 based peptides may be a possible treatment for involuntary muscle spasms (Hodel 1998).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Mouse
Dog Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human SNAP-25 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Peptide sequence C-DEANQRATKMLGSG corresponding to the C-terminus of SNAP-25 (NP_003072.2, NP_570824.1).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P60880
Entrez Gene
SNAP25
Regulatory
For research purposes only

Applications of SNAP-25 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/32000
Immunohistology - Paraffin 1 2.0 4.0ug/ml
Western Blotting 0.01 0.03ug/ml
  1. 1Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. Bio-Rad recommend citrate buffer, pH6.0, for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1124 detects a band of approximately 22kDa in human and mouse brain lysates. A minimum incubation time of 1 hour is recommended with this antibody.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Product Specific References

References for SNAP-25 antibody

  1. Hodel, A. (1998) SNAP-25.
    Int J Biochem Cell Biol. 30 (10): 1069-73.

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