Pericentrin 1 antibody

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Goat anti Human Pericentrin 1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1092 E WB 0.1 mg
Goat anti Human Pericentrin 1 antibody recognises the C-terminal of human pericentrin 1, a ~75 kDa component of the filamentous matrix of the centrosome, expressed in all tissues. When associated with centrosome and Golgi localised protein kinase N-associated protein (CG-NAP), pericentrin 1 anchors the gamma-tubulin ring complex, providing a site for microtubule nucleation and the initial establishment of organized microtubule arrays. It binds calmodulin and is a constituent of nuclear pore proteins (nup) 107-160, a sub-complex of the nuclear pore complex. Pericentrin 1 is thought to play a role in the functioning of the centrosomes, cytoskeleton, and cell-cycle progression.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human pericentrin 1 (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Peptide sequence C-ARAIIREGSLEGS corresponding to the C-terminal of pericentrin 1 (NP_079120).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9BW27 Related reagents
Entrez Gene
NUP85 Related reagents
GO Terms
GO:0005515 protein binding
GO:0000236 mitotic prometaphase
GO:0000777 condensed chromosome kinetochore
GO:0005829 cytosol
GO:0005819 spindle
GO:0005975 carbohydrate metabolic process
GO:0016032 viral reproduction
GO:0010827 regulation of glucose transport
GO:0015031 protein transport
GO:0015758 glucose transport
GO:0031080 Nup107-160 complex
GO:0031965 nuclear membrane
GO:0051028 mRNA transport
GO:0055085 transmembrane transport
Regulatory
For research purposes only

Applications of Pericentrin 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA >1/32,000
Western Blotting 1 3ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1092 detects a band of approximately 75kDa in Hela cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB