NLRX1 antibody

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Goat anti NLRX1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP3022
Datasheet
WB
SDS
0.1 mg

Goat anti NLRX1 antibody recognizes NLR family member X1 (NLRX1), also known as caterpiller protein 11.3, NOD26, NOD5, and NOD9.

NLRX1 is a member of the NLR family of pattern recognition receptors (PRRs), but is an atypical member due to its structure (Nagai-Singer et al. 2019). For example, it appears to be the only PRR family member with the ability to localize to mitochondria (Chu et al. 2019). However, NLRX1 appears to be ubituitously expressed in human cells, and appears to have different functions depending on cell type (Nagai-Singer et al. 2019). NLRX1 appears to be a negative regulator of immune signaling during microbial infection, cancer, and autoimmune disease (Kastelberg et al. 2020). Consistent with mitochondrial localization, NLRX1 regulates the function of mitochondria during apoptosis and injury in the presence of TNF-alpha (Singh et al. 2019).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human NLRX1 were raised by repeated immunizations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence C-NFSGETLDSTDPSN, from the internal region of the protein sequence according to NP_078894.2; NP_733840.1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q86UT6
Entrez Gene
NLRX1
GO Terms
GO:0005524 ATP binding
GO:0005741 mitochondrial outer membrane
GO:0032480 negative regulation of type I interferon production
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of NLRX1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 ug/ml 3 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Goat anti Human NLRX1 antibody detects a band of approximately 100 kDa in cell lysates

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

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