mTOR antibody

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Rabbit anti mTOR

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00174
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human mTOR antibody recognizes the Serine/threonine-protein kinase mTOR, also known as FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, mammalian target of rapamycin, mechanistic target of rapamycin, rapamycin, FKBP12 target 1 and rapamycin target protein 1.

mTOR belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein, encoded by the MTOR (FRAP1) gene, acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of MTOR (provided by RefSeq, Sep 2008).

Rabbit anti Human mTOR antibody detects a band of 273 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti mTOR detects a band of approximately 273 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Synthetic peptide corresponding to amino acids 2440-2457 of human mTOR

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P42345 Related reagents
Entrez Gene
MTOR Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0016049 cell growth
GO:0005764 lysosome
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005741 mitochondrial outer membrane
GO:0005789 endoplasmic reticulum membrane
GO:0005942 phosphatidylinositol 3-kinase complex
GO:0007584 response to nutrient
GO:0008286 insulin receptor signaling pathway
GO:0018105 peptidyl-serine phosphorylation
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030163 protein catabolic process
GO:0031295 T cell costimulation
GO:0031929 TOR signaling cascade
GO:0031931 TORC1 complex
GO:0031932 TORC2 complex
GO:0043200 response to amino acid stimulus
GO:0046777 protein autophosphorylation
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051219 phosphoprotein binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of mTOR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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