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MEK 1 antibody

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Anti MEK 1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti MEK 1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2 Anti MEK 1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3
Anti MEK 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with MEK 1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to MEK 1 (molecular weight 48 kDa)
Anti MEK 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Western blot analysis of MEK1 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with Rabbit anti MEK1 antibody (VPA00907) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and visualized on the ChemiDoc MP with a 583 second exposure. Tubulin was detected with hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 30 second exposure.
Anti MEK 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3
Normalized signal intensity of MEK1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Rabbit anti MEK 1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
MEK 1

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Product Code Applications Pack Size List Price Your Price Qty
VPA00907
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader

Rabbit anti Human MEK 1 antibody recognizes MEK 1, also known as MAP2K1, CFC3, ERK activator kinase 1 and dual specificity mitogen-activated protein kinase kinase 1.

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (provided by RefSeq, Jul 2008).

Rabbit anti Human MEK 1 antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti MEK 1 antibody recognizes a band of approximately 48 kDa in A431 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Synthetic peptide of human MEK 1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P E WB 2 ml loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
Q02750
Entrez Gene
MAP2K1
GO Terms
GO:0005886 plasma membrane
GO:0000186 activation of MAPKK activity
GO:0000187 activation of MAPK activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0004708 MAP kinase kinase activity
GO:0004713 protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0007050 cell cycle arrest
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008063 Toll signaling pathway
GO:0008285 negative regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051403 stress-activated MAPK cascade
GO:0090398 cellular senescence

VPA00907

180704

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