MEK 1 antibody

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Anti MEK 1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti MEK 1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti MEK 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with MEK 1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to MEK 1 (molecular weight 48 kDa)

Anti MEK 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of MEK1 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with Rabbit anti MEK1 antibody (**VPA00907**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and visualized on the ChemiDoc MP with a 583 second exposure. Tubulin was detected with hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 30 second exposure.

Anti MEK 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of MEK1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Rabbit anti MEK 1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VPA00907 Datasheet	WB SDS	100 µl	Log in		Get Quote

Rabbit anti Human MEK 1 antibody recognizes MEK 1, also known as MAP2K1, CFC3, ERK activator kinase 1 and dual specificity mitogen-activated protein kinase kinase 1.

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (provided by RefSeq, Jul 2008).

Rabbit anti Human MEK 1 antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti MEK 1 antibody recognizes a band of approximately 48 kDa in A431 cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Rabbit polyclonal antibody purified by affinity chromatography on immunogen

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide
<50% Glycerol

Immunogen

Synthetic peptide of human MEK 1

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Storage Information

Storage: Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee: 12 months from date of despatch

More Information

UniProt: Q02750
Entrez Gene: MAP2K1
GO Terms: GO:0005886 plasma membrane; GO:0000186 activation of MAPKK activity; GO:0000187 activation of MAPK activity; GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0004708 MAP kinase kinase activity; GO:0004713 protein tyrosine kinase activity; GO:0005524 ATP binding; GO:0007050 cell cycle arrest; GO:0007173 epidermal growth factor receptor signaling pathway; GO:0007265 Ras protein signal transduction; GO:0007411 axon guidance; GO:0008063 Toll signaling pathway; GO:0008285 negative regulation of cell proliferation; GO:0008286 insulin receptor signaling pathway; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0045087 innate immune response; GO:0048011 nerve growth factor receptor signaling pathway; GO:0051403 stress-activated MAPK cascade; GO:0090398 cellular senescence
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of MEK 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in		Get Quote

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