Lambda Light Chain antibody

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Goat F(ab')2 anti Human Lambda Light Chain:FITC

Goat F(ab')2 anti Human Lambda Light Chain:HRP

Goat F(ab')2 anti Human Lambda Light Chain

Goat F(ab')2 anti Human Lambda Light Chain:RPE

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR130F C F IF 0.5 mg
STAR130P C E P WB 1 ml
STAR130 E 0.5 mg
STAR130PE F 0.5 ml
Goat F(ab')2 anti Human Lambda Light Chain antibody recognizes human lambda light chains. This antibody has been cross absorbed against pooled human myeloma proteins with kappa light chains.

Product Details

Target Species
Human
Product Form
F(ab')2 fragments of purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
F(ab')2 fragments of purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
F(ab')2 fragments of purified IgG - liquid
Product Form
F(ab')2 fragments of purified IgG conjugated to R. Phycoerythrin - liquid
Antiserum Preparation
Antisera to human lambda chains were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
F(ab')2 fragments were prepared by pepsin digestion.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
50%Glycerol
0.01%Thiomersal
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
Sucrose
Immunogen
Human Iambda light chains.
Approx. Protein Concentrations
F(ab')2 concentration 0.5mg/ml
Approx. Protein Concentrations
F(ab')2 concentration 0.5mg/ml
Approx. Protein Concentrations
F(ab')2 concentration 0.25mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
6 months from date of despatch.

More Information

UniProt
P0CG04 Related reagents
Entrez Gene
IGLV2-14 Related reagents
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of Lambda Light Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Immunofluorescence Neat 1/10
Immunohistology - Frozen
ELISA 1/4000 1/8000
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
ELISA
Flow Cytometry 1/20 1/30
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F