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Kappa Light Chain antibody

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Goat F(ab')2 anti Human Kappa Light Chain:Biotin

Goat F(ab')2 anti Human Kappa Light Chain:HRP

Goat F(ab')2 anti Human Kappa Light Chain

Goat F(ab')2 anti Human Kappa Light Chain:RPE

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
STAR128B
Datasheet
E IF WB
SDS
0.5 mg loader

STAR128P
Datasheet
C E P WB
SDS
1 ml loader

STAR128
Datasheet
E
SDS
0.5 mg loader

STAR128PE
Datasheet
F
SDS
0.5 ml loader

Goat F(ab')2 anti Human Kappa Light Chain antibody recognizes human kappa light chains. This antibody has been cross absorbed with pooled human myeloma proteins with lambda light chains.

Product Details

Target Species
Human
Product Form
F(ab)2 fragments of purified IgG conjugated to Biotin - liquid
Product Form
F(ab')2 fragment of IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
F(ab)2 fragments of purified IgG - liquid
Product Form
F(ab)2 fragments of purified IgG conjugated to R. Phycoerythrin - liquid
Antiserum Preparation
Antisera to human kappa chains were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on pooled human Igs with kappa light chains covalently linked to agarose. F(ab)2 fragments were prepared by pepsin digestion.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.01% Thiomersal
50% Glycerol
Preservative Stabilisers
None present
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Stabilizing agent
Immunogen
Human kappa light chains.
Approx. Protein Concentrations
F(ab')2 concentration 0.5mg/ml
Approx. Protein Concentrations
F(ab')2 concentration 0.5 mg/ml
Approx. Protein Concentrations
F(ab')2 concentration 0.25 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
12 months from date of despatch
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
6 months from date of despatch

More Information

UniProt
P01834
Entrez Gene
IGKC
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of Kappa Light Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/20000
Immunofluorescence
Western Blotting
ELISA 1/4000 1/8000
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
ELISA
Flow Cytometry 1/20 1/30
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

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Description Product Code Applications Pack Size List Price Quantity
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