Kappa Light Chain antibody
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Goat F(ab')2 anti Human Kappa Light Chain:Biotin
Goat F(ab')2 anti Human Kappa Light Chain:HRP
Goat F(ab')2 anti Human Kappa Light Chain
Goat F(ab')2 anti Human Kappa Light Chain:RPE
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| STAR128B | E IF WB | 0.5 mg |
|
|
| STAR128P | C E P WB | 1 ml |
|
|
| STAR128 | E | 0.5 mg | ||
| STAR128PE | F | 0.5 ml |
|
Goat F(ab')2 anti Human Kappa Light Chain antibody recognizes human kappa light chains. This antibody has been cross absorbed with pooled human myeloma proteins with lambda light chains.
Product Details
- Target Species
- Human
- Product Form
- F(ab)2 fragments of purified IgG conjugated to Biotin - liquid
- Product Form
- F(ab')2 fragment of IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- F(ab)2 fragments of purified IgG - liquid
- Product Form
- F(ab)2 fragments of purified IgG conjugated to R. Phycoerythrin - liquid
- Antiserum Preparation
- Antisera to human kappa chains were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on pooled human Igs with kappa light chains covalently linked to agarose. F(ab)2 fragments were prepared by pepsin digestion.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Borate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.01% Thiomersal
50% Glycerol - Preservative Stabilisers
- None present
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose) - Immunogen
- Human kappa light chains.
- Approx. Protein Concentrations
- F(ab')2 concentration 0.5mg/ml
- Approx. Protein Concentrations
- F(ab')2 concentration 0.5 mg/ml
- Approx. Protein Concentrations
- F(ab')2 concentration 0.25 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- 12 months from date of despatch
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- 6 months from date of despatch
More Information
- UniProt
- P01834
- Entrez Gene
- IGKC
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
- Regulatory
- For research purposes only
Applications of Kappa Light Chain antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/5000 | 1/20000 | |
| Immunofluorescence | |||
| Western Blotting | |||
| ELISA | 1/4000 | 1/8000 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting | |||
| ELISA | |||
| Flow Cytometry | 1/20 | 1/30 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
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