IL-6

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  • Recombinant Human Interleukin-6
  • Recombinant Human Interleukin-6
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP045E, FN, WBdatasheet pdfdatasheet pdf20 µg
    PHP045
    PHP045AE, FN, WBdatasheet pdfdatasheet pdf5 µg
    PHP045A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Recombinant Human Interleukin-6 protein produced in E. coli constitutes full length non-glycosylated human IL-6 and retainf full bioogical activity. Interleukin-6 is a potent lymphoid cell growth factor, acting on B cells, T cells and hybridoma cells.This preparation has an ED50 of 0.1ng/ml when assayed by proliferation of murine 7TD1 cells.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays0.110ng/ml
      Western Blotting1.53.0ng/lane
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays0.110ng/ml
      Western Blotting1.53.0ng/lane

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      Recombinant Human IL-6 may be used as a standard for ELISA applications with Rabbit anti Human Interleukin-6 for detection.
    • ELISA
      PHP045A may be used as the standard in ELISA applications with either AHP1040 or AHP1040B.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      Recombinant Human IL-6 may be used as the positive control for western blotting applications with Rabbit anti Human Interleukin-6 for detection.
    • Western Blotting
      PHP045A may be used as a positive control for western blotting applications with AHP1040 or AHP1040B.
    • Instructions For Use
    • Instructions For Use

    Additional IL-6 Formats

    Formats Applications Sizes available
    IL-6 : Rec. Protein E, FN, WB 20 µg | 5 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human Interleukin-6:BiotinAHP1040B50 µgE, WB
          AHP1040B
          Rabbit anti Human Interleukin-6AHP10400.1 mgE, FN*, IF, P *, WB
          AHP1040
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human Interleukin-6:BiotinAHP1040B50 µgE, WB
          AHP1040B
          Rabbit anti Human Interleukin-6AHP10400.1 mgE, FN*, IF, P *, WB
          AHP1040

          Recommended Positive Controls

            Histology Controls

              References

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