IL-18 antibody
Rabbit anti Interleukin-18
Rabbit anti-human IL-18 antibody recognizes interleukin-18, also known as IFN-gamma-inducing factor, IL-1 gamma or iboctadekin. This antibody has been extensively validated for western blotting using whole cell lysates.
Interleukin-18 Antibody with Control Lysate
Rabbit anti-human IL-18 antibody recognizes interleukin-18, also known as IFN-gamma-inducing factor, IL-1 gamma or iboctadekin. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00455 | WB | 100 µl | ||||
| VPA00455KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human interleukin-18 antibody recognizes interleukin-18 (IL-18), also known as IFN-gamma-inducing factor, IL-1 gamma or iboctadekin.
The protein encoded by IL18 gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for IL-18 (provided by RefSeq, Aug 2011).
Rabbit anti Human interleukin-18 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by IL18 gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for IL-18 (provided by RefSeq, Aug 2011).
Rabbit anti Human interleukin-18 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti IL-18 detects a band of approximately 22 kDa in HeLa cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide directed towards the C terminal region of human interleukin-18
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q14116 Related reagents
- Entrez Gene
- IL18 Related reagents
- GO Terms
- GO:0001525 angiogenesis
- GO:0005829 cytosol
- GO:0004871 signal transducer activity
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0007267 cell-cell signaling
- GO:0030155 regulation of cell adhesion
- GO:0030431 sleep
- GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production
- GO:0032729 positive regulation of interferon-gamma production
- GO:0032740 positive regulation of interleukin-17 production
- GO:0032819 positive regulation of natural killer cell proliferation
- GO:0034105 positive regulation of tissue remodeling
- GO:0042033 chemokine biosynthetic process
- GO:0042088 T-helper 1 type immune response
- GO:0042092 type 2 immune response
- GO:0042094 interleukin-2 biosynthetic process
- GO:0042095 interferon-gamma biosynthetic process
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0042231 interleukin-13 biosynthetic process
- GO:0042253 granulocyte macrophage colony-stimulating factor biosynthetic process
- GO:0050729 positive regulation of inflammatory response
- GO:0051142 positive regulation of NK T cell proliferation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of IL-18 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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