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IL-18 antibody

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Western Blot Validated
Anti Interleukin-18 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with interleukin-18 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 41 second exposure. Arrow points to interleukin-18 (molecular weight 22 kDa).

Rabbit anti Interleukin-18

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00455
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human interleukin-18 antibody recognizes interleukin-18 (IL-18), also known as IFN-gamma-inducing factor, IL-1 gamma or iboctadekin.

The protein encoded by IL18 gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for IL-18 (provided by RefSeq, Aug 2011).

Rabbit anti Human interleukin-18 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti IL-18 detects a band of approximately 22 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the C terminal region of human interleukin-18

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q14116
Entrez Gene
IL18
GO Terms
GO:0001525 angiogenesis
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0007267 cell-cell signaling
GO:0030155 regulation of cell adhesion
GO:0030431 sleep
GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production
GO:0032729 positive regulation of interferon-gamma production
GO:0032740 positive regulation of interleukin-17 production
GO:0032819 positive regulation of natural killer cell proliferation
GO:0034105 positive regulation of tissue remodeling
GO:0042033 chemokine biosynthetic process
GO:0042088 T-helper 1 type immune response
GO:0042092 type 2 immune response
GO:0042094 interleukin-2 biosynthetic process
GO:0042095 interferon-gamma biosynthetic process
GO:0042104 positive regulation of activated T cell proliferation
GO:0042231 interleukin-13 biosynthetic process
GO:0042253 granulocyte macrophage colony-stimulating factor biosynthetic process
GO:0050729 positive regulation of inflammatory response
GO:0051142 positive regulation of NK T cell proliferation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of IL-18 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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