IL-17 antibody

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Rabbit anti Human Interleukin-17

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP455G
Datasheet
E FN* IF P* WB
MSDS
0.1 mg loader

Rabbit anti Human Interleukin-17 antibody recognizes human IL-17 (Interleukin-17/17A), also known as Cytotoxic T-lymphocyte-associated antigen 8 or CTLA-8. Interleukin-17 is a homodimer of two 132 amino acid chains resulting oin a ~32 kDa disulfide-linked cytokine belonging to the IL-17 family (classified as IL-17A to IL-17F) which signals through the IL-17 receptor (IL-17R/CDw217).

IL-17 is produced primarily by activated CD4+ T cells and induces a variety of target cells to secrete inflammatory cytokines, including IL-1beta, IL-6, TNF-α, IFN-&gamma, and granulocyte colony-stimulating factor, as well as synergizing with other inflammatory cytokines and agonists. The role of IL-17 in bone metabolism has become a focus area for studies into Rheumatoid Arthritis, in which levels of IL-17 are significantly increased and may contribute to bone destruction (Ziolkowska et al. 2000).

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to human IL-17 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human IL-17
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
6 months from date of despatch

More Information

UniProt
Q16552
Entrez Gene
IL17A
GO Terms
GO:0001775 cell activation
GO:0006915 apoptosis
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006486 protein glycosylation
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0032747 positive regulation of interleukin-23 production
GO:0045672 positive regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
Regulatory
For research purposes only

Applications of IL-17 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 1 0.93 1.3ug/ml
Immunofluorescence
Immunohistology - Paraffin 2 0.25ug/ml
Western Blotting 0.1 0.2ug/ml
  1. 1 To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17A (50.0 ng/ml).
  2. 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This purified human IL-17 antibody may be used in an ELISA with recombinant human IL-17 (PHP098) as the standard.
Western Blotting
This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IL-17 (PHP098) as the positive control.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Positive Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Recombinant Human Interleukin-17 PHP098 E FN WB 25 µg loader

Application Based External Images

Immunofluorescence

Product Specific References

References for IL-17 antibody

  1. Parsonage, G. et al. (2008) Prolonged, granulocyte-macrophage colony-stimulating factor-dependent, neutrophil survival following rheumatoid synovial fibroblast activation by IL-17 and TNFalpha.
    Arthritis Res Ther. 10: R47.

Further Reading

  1. Gaffen, S.L. (2004) Biology of recently discovered cytokines: interleukin-17--a unique inflammatory cytokine with roles in bone biology and arthritis.
    Arthritis Res Ther. 6 (6): 240-7.