IL-17 antibody
Rabbit anti Human Interleukin-17
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-17
Antibody Quality and Performance Guarantee
| Rabbit anti Human Interleukin-17 antibody recognizes human IL-17 (Interleukin-17/17A), also known as Cytotoxic T-lymphocyte-associated antigen 8 or CTLA-8. Interleukin-17 is a homodimer of two 132 amino acid chains resulting oin a ~32 kDa disulfide-linked cytokine belonging to the IL-17 family (classified as IL-17A to IL-17F) which signals through the IL-17 receptor (IL-17R/CDw217). IL-17 is produced primarily by activated CD4+ T cells and induces a variety of target cells to secrete inflammatory cytokines, including IL-1beta, IL-6, TNF-α, IFN-&gamma, and granulocyte colony-stimulating factor, as well as synergizing with other inflammatory cytokines and agonists. IL-17 has roles in bone metabolism and rheumatoid arthritis, in which levels of IL-17 are significantly increased and may contribute to bone destruction (Ziolkowska et al. 2000). |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to human IL-17 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-17
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays 1 | |
0.93 | 1.3ug/ml |
| Immunofluorescence | |
||
| Immunohistology - Paraffin 2 | |
0.25ug/ml | |
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1 To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17A (50.0 ng/ml).
- 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This purified human IL-17 antibody may be used in an ELISA with recombinant human IL-17 (PHP098) as the standard.
- Western Blotting
- This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IL-17 (PHP098) as the positive control.
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Recombinant Human Interleukin-17 | PHP098 | E FN WB | 25 µg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Recombinant Human Interleukin-17 | ||||||
References for IL-17 antibody
-
Parsonage, G. et al. (2008) Prolonged, granulocyte-macrophage colony-stimulating factor-dependent, neutrophil
survival following rheumatoid synovial fibroblast activation by IL-17 and TNFalpha.
Arthritis Res Ther. 10: R47.
Further Reading
-
Gaffen, S.L. (2004) Biology of recently discovered cytokines: interleukin-17--a unique inflammatory cytokine with roles in bone biology and arthritis.
Arthritis Res Ther. 6 (6): 240-7.
- Synonyms
- IL-17A
- RRID
- AB_2124883
- UniProt
- Q16552
- Entrez Gene
- IL17A
- GO Terms
- GO:0001775 cell activation
- GO:0006915 apoptosis
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006486 protein glycosylation
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007267 cell-cell signaling
- GO:0032747 positive regulation of interleukin-23 production
- View More GO Terms
- GO:0045672 positive regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
AHP455G
150811
157678
159670
161708
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