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  • Recombinant Human Interleukin-17
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP098E, FN, WBdatasheet pdfdatasheet pdf25 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Interleukin-17 is a disulphide-linked homodimer of two 15.5kDa subunits. High levels of IL-17 homodimer are produced by stimulated primary CD4 the T-cells from the peripheral blood. IL-17 is reported to enhance the expression of intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts.
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein expressed in E. coli - lyophilised
    • Reconstitution
      Reconstitute with 25 ul distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
    • Preservative Stabilisers
      None present
    • Purity
      >98% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      1.0 mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1 ng/ug
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      protein glycosylation
      positive regulation of transcription from RNA polymerase II promoter
      extracellular space
      cell-cell signaling
      cell activation
      positive regulation of interleukin-23 production
      immune response
      cytokine activity
      positive regulation of osteoclast differentiation
      inflammatory response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays1ng/ml2ng/ml
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      PHP098 may be used as a standard in a sandwich ELISA with AHP455G as the capture antibody and AHP455B as the detection antibody.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      In Western blotting PHP098 represents as suitable positive control for use with AHP455G or AHP455B.
    • Instructions For Use

    Additional IL-17 Formats

    Formats Applications Sizes available
    IL-17 : Rec. Protein E, FN, WB 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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