IgG IgA IgM antibody
Horseradish peroxidase conjugated Goat anti Human IgG/A/M antibody (STAR90P) used as a detection reagent for the measurement of total antibody response to tuberculoid antigens by ELISA.
Image caption:
Compared to healthy subjects, TB patients display diminution in antibody levels against PhoP but not ESAT-6, CFP-10 and Acr1.
Antibodies were measured in the serum of healthy, TB patients and close contacts against (A) PhoP; (B) Acr1; (C) ESAT-6 and (D) CFP-10. Median with 95% Cl represent the antibodies titers and each dot symbolizes single individual (N: number of individuals). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns: non-significant.
From: Vidyarthi A, Khan N, Agnihotri T, Siddiqui KF, Nair GR, Arora A, et al. (2017)
Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.
PLoS ONE 12(3): e0173769.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Human IgG/A/M antibody (STAR90P) used as a detection reagent for the measurement of total antibody response to tuberculoid antigens by ELISA.
Image caption:
PhoP showed maximum antibody titer compared to ESAT-6, CFP-10 and Acr-1.
Antibodies against PhoP, Acr1, ESAT-6 and CFP-10 were measured in the serum of (A) patients; (B) contacts; (C) healthy. Median with 95% Cl represent the antibodies titers and each dot symbolizes single individual (N: number of individuals). **p<0.01, ***p<0.001, ****p<0.0001, ns: non-significant.
From: Vidyarthi A, Khan N, Agnihotri T, Siddiqui KF, Nair GR, Arora A, et al. (2017)
Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.
PLoS ONE 12(3): e0173769.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Human IgG/A/M antibody (STAR90P) used as a detection reagent for the measurement of total antibody response to tuberculoid antigens by ELISA.
Image caption:
Ratio of Acr-1/PhoP antibody titer, discriminates among patients, contacts and healthy subjects.
Antibodies ratio of (A) Acr-1/PhoP; (B) PhoP/Acr-1 were measured using the antibody titer against PhoP and Acr-1 in the serum of healthy, TB patients and close contacts. Median with 95% Cl represent the antibodies ratio between two antigens and each dot symbolizes single individual (N: number of individuals). *p<0.05, ****p<0.0001.
From: Vidyarthi A, Khan N, Agnihotri T, Siddiqui KF, Nair GR, Arora A, et al. (2017)
Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.
PLoS ONE 12(3): e0173769.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Human IgG/A/M antibody (STAR90P) used as a detection reagent for the measurement of total antibody response to tuberculoid antigens by ELISA.
Image caption:
Relapsed cases show lower anti-PhoP antibodies than healthy volunteers.
Antibodies against PhoP, Acr-1, ESAT-6 and CFP-10 were measured in the serum of healthy individuals, patients and relapsed cases. Median with 95% Cl represent the antibodies titers and each dot symbolizes single individual (N: number of individuals). *p<0.05, ****p<0.0001, ns: non-significant.
From: Vidyarthi A, Khan N, Agnihotri T, Siddiqui KF, Nair GR, Arora A, et al. (2017)
Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.
PLoS ONE 12(3): e0173769.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Human IgG/A/M antibody (STAR90P) used as a detection reagent for the measurement of total antibody response to tuberculoid antigens by ELISA.
Image caption:
Diabetic TB patients show lower anti-ESAT-6 antibody response compared to non-diabetic TB patients.
Antibodies against PhoP, Acr-1, ESAT-6 and CFP-10 were measured in the serum of diabetic TB patients and non-diabetic TB patients. Median with 95% Cl represent the antibodies titers and each dot symbolizes single individual (N: number of individuals).
From: Vidyarthi A, Khan N, Agnihotri T, Siddiqui KF, Nair GR, Arora A, et al. (2017)
Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.
PLoS ONE 12(3): e0173769.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Human IgG/A/M:FITC
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG IgA IgM
Antibody Quality and Performance Guarantee
| Goat anti Human IgG/A/M antibody recognizes human Ig, binding to all major classes (IgG, IgA, IgM). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Antiserum Preparation
- Antisera to human Ig were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Human IgG, IgA and IgM whole molecules
- Approx. Protein Concentrations
- IgG concentration 2.0 mg/ml after reconstitution
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.04 ug/ml | 0.2 ug/ml |
| Flow Cytometry | |
0.1 ug/ml | 0.5 ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
References for IgG IgA IgM antibody
-
Lierz, M. et al. (2008) Avian Mycoplasma lipofaciens transmission to veterinarian.
Emerg Infect Dis. 14: 1161-3. -
Vidyarthi, A. et al. (2017) Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.
PLoS One. 12 (3): e0173769. -
Jaki, T. et al. (2016) A false sense of security? Can tiered approach be trusted to accurately classify immunogenicity samples?
J Pharm Biomed Anal. 128: 166-73.
- RRID
- AB_567009
- UniProt
- P01859
- P01860
- P01857
- P01861
- P01876
- P01877
- P01871
- Entrez Gene
- IGHG1
- IGHG2
- IGHG3
- IGHG4
- IGHM
- IGHA1
- IGHA2
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0006955 immune response
- GO:0016021 integral to membrane
- GO:0018298 protein-chromophore linkage
View more products with IgG specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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