IgG IgA IgM antibody

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Goat anti Human IgG/A/M:FITC

Goat anti Human IgG/A/M:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR90F E F 2 mg
STAR90P E WB 2 mg
Goat anti Human IgG/A/M antibody recognizes human Ig, binding to all major classes (IgG, IgA, IgM).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilised
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Reconstitution
Reconstitute with 2ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to human Ig were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.01%gentamicin sulphate
1%Bovine Serum Albumin
Immunogen
Human IgG, IgA and IgM whole molecules
Approx. Protein Concentrations
IgG concentration 2.0 mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P01859 Related reagents
P01860 Related reagents
P01857 Related reagents
P01861 Related reagents
P01876 Related reagents
P01877 Related reagents
P01871 Related reagents
Entrez Gene
IGHG1 Related reagents
IGHG2 Related reagents
IGHG3 Related reagents
IGHG4 Related reagents
IGHM Related reagents
IGHA1 Related reagents
IGHA2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
GO:0016021 integral to membrane
GO:0018298 protein-chromophore linkage
Regulatory
For research purposes only

Applications of IgG IgA IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.04 ug/ml 0.2 ug/ml
Flow Cytometry 0.1 ug/ml 0.5 ug/ml
ELISA 1/10,000 1/50,000
Western Blotting 1/1,000 1/5,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for IgG IgA IgM antibody

  1. Lierz, M. et al. (2008) Avian Mycoplasma lipofaciens transmission to veterinarian.
    Emerg Infect Dis. 14: 1161-3.
  2. Vidyarthi, A. et al. (2017) Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.
    PLoS One. 12 (3): e0173769.
  3. Jaki, T. et al. (2016) A false sense of security? Can tiered approach be trusted to accurately classify immunogenicity samples?
    J Pharm Biomed Anal. 128: 166-73.