IgG IgA IgM antibody

Goat anti Human IgG/A/M:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG IgA IgM

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Goat anti Human IgG/A/M antibody recognizes human Ig, binding to all major classes (IgG, IgA, IgM).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to human Ig were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Human IgG, IgA and IgM whole molecules
Approx. Protein Concentrations
IgG concentration 2.0 mg/ml after reconstitution
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.04 ug/ml 0.2 ug/ml
Flow Cytometry 0.1 ug/ml 0.5 ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for IgG IgA IgM antibody

  1. Lierz, M. et al. (2008) Avian Mycoplasma lipofaciens transmission to veterinarian.
    Emerg Infect Dis. 14: 1161-3.
  2. Vidyarthi, A. et al. (2017) Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.
    PLoS One. 12 (3): e0173769.
  3. Jaki, T. et al. (2016) A false sense of security? Can tiered approach be trusted to accurately classify immunogenicity samples?
    J Pharm Biomed Anal. 128: 166-73.

RRID
AB_567009
UniProt
P01859
P01860
P01857
P01861
P01876
P01877
P01871
Entrez Gene
IGHG1
IGHG2
IGHG3
IGHG4
IGHM
IGHA1
IGHA2
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
GO:0016021 integral to membrane
GO:0018298 protein-chromophore linkage
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products