IGFBP-7 antibody
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Rabbit anti Human IGFBP-7 antibody recognizes human IGFBP-7 (insulin-like growth factor binding protein-7), also known as mac25, a 256 amino acid secreted protein and member of a growing family of IGFBPs which regulate the biological activities and distribution of the insulin-like growth factors (IGFs), through binding to IGF-I and IGF-II.
IGFBP-7 is widely expressed throughout normal tissues, but is also expressed in many tumour types at an increased or reduced level, and acts as a suppressor of breast and prostate cancer cell lines. IGFBP-7 inhibits IGF-induced differentiation of skeletal muscle (myogenesis) and has implications in bone formation, by its possible affects on the actions of parathyroid hormone (PTH) and cortisol.
IGFBP-7 is widely expressed throughout normal tissues, but is also expressed in many tumour types at an increased or reduced level, and acts as a suppressor of breast and prostate cancer cell lines. IGFBP-7 inhibits IGF-induced differentiation of skeletal muscle (myogenesis) and has implications in bone formation, by its possible affects on the actions of parathyroid hormone (PTH) and cortisol.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.
- Antiserum Preparation
- Antisera to human IGFBP-7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IGFBP-7
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 6 months from date of despatch
More Information
- UniProt
- Q16270
- Entrez Gene
- IGFBP7
- GO Terms
- GO:0001558 regulation of cell growth
- GO:0007155 cell adhesion
- GO:0005520 insulin-like growth factor binding
- GO:0005615 extracellular space
- GO:0008285 negative regulation of cell proliferation
- Regulatory
- For research purposes only
Applications of IGFBP-7 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 | 2.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This purified human IGFBP-7 antibody may be used in an ELISA recombinant human IGFBP-7 (PHP178) as the standard.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Ruan, W.J. et al. (2006) IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis with its expression associated with DNA hypomethylation of exon 1.
J Zhejiang Univ Sci B. 7 (11): 929-32. -
Pereira, R.C. & Canalis, E. (1999) Parathyroid hormone increases mac25/insulin-like growth factor-binding protein-related protein-1 expression in cultured osteoblasts.
Endocrinology. 140 (5): 1998-2003.
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