IGFBP-7

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  • Recombinant Human IGFBP-7
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP178E, WBdatasheet pdfdatasheet pdf25 µg
    PHP178
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • IGFBP-7 (insulin-like growth factor binding protein-7) is a secreted protein which belongs to a family of IGF-BPs which regulate the biological activities and distribution of the insulin-like growth factors (IGFs), through binding to IGF-I and IGF-II. IGFBP-7 is expressed in many tumour types and acts as a suppressor of breast and prostate cancer cell lines. IGFBP-7 inhibits IGF-induced differentiation of skeletal muscle (myogenesis) and has implications in bone formation.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.25 ml distilled water.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage the additiona of 0.1% BSA is recommended.
    • Preparation
      Purified recombinant IGFBP-7 expressed in E.coli.
    • Preservative Stabilisers
      None present
    • Purity
      >98% by SDS PAGE and HPLC analysis.
    • Approx. Protein Concentrations
      0.1 mg/ml after reconstitution
    • Molecular Weight
      26.4kD (257 amino acid residues).
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <1EU/ug
    • Test Principle
    • Buffer Solution
      10mM Sodium Phosphate, pH6.8
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      cell adhesion
      extracellular space
      regulation of cell growth
      negative regulation of cell proliferation
      insulin-like growth factor binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Western Blotting1.53.0ng/lane

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This recombinant protein may be used as a standard in a sandwich ELISA with AHP1282 as a capture reagent and AHP1282B as the detection reagent.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      This recombinant protein may be as a positive control in western blotting for AHP1282 and may be run under either reducing or non-reducing conditions.
    • Instructions For Use

    Additional IGFBP-7 Formats

    Formats Applications Sizes available
    IGFBP-7 : Rec. Protein E, WB 25 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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