IGFBP-7 (insulin-like growth factor binding protein-7) is a secreted protein which belongs to a family of IGF-BPs which regulate the biological activities and distribution of the insulin-like growth factors (IGFs), through binding to IGF-I and IGF-II. IGFBP-7 is expressed in many tumour types and acts as a suppressor of breast and prostate cancer cell lines. IGFBP-7 inhibits IGF-induced differentiation of skeletal muscle (myogenesis) and has implications in bone formation.
Purified recombinant protein - lyophilised
Reconstitute with 0.25 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage the additiona of 0.1% BSA is recommended.
Purified recombinant IGFBP-7 expressed in E.coli.
>98% by SDS PAGE and HPLC analysis.
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
26.4kD (257 amino acid residues).
Reagents In The Kit
Preparing The Antibody
10mM Sodium Phosphate, pH6.8
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
This recombinant protein may be used as a standard in a sandwich ELISA with AHP1282 as a capture reagent and AHP1282B as the detection reagent.
Histology Positive Control Tissue
This recombinant protein may be as a positive control in western blotting for AHP1282 and may be run under either reducing or non-reducing conditions.