IgE antibody
Goat anti Human IgE:FITC
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgE
Antibody Quality and Performance Guarantee
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E Reset| Goat anti Human IgE polyclonal antibody recognizes human IgE. Minimal cross-reactivity (<0.1%) is expected with other human immunoglobulins and light chains. Goat anti Human IgE in a biotinylated format has been successfully employed as a detection reagent in the development of a sensitive sandwich ELISA for the measurement of human IgE. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography.
- Antiserum Preparation
- Antisera to human IgE were raised by repeated immunisations of goats with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Immunogen
- Native human IgE.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
References for IgE antibody
-
Khan, F.M. et al. (2012) Basophil activation test compared to skin prick test and fluorescence enzyme immunoassay for aeroallergen-specific Immunoglobulin-E.
Allergy Asthma Clin Immunol. 8 (1): 1. -
Pinot de Moira, A. et al. (2013) Effects of treatment on IgE responses against parasite allergen-like proteins and immunity to reinfection in childhood schistosome and hookworm coinfections.
Infect Immun. 81 (1): 23-32. -
Zheng, W. et al. (2016) Upregulated expression of substance P in basophils of the patients with chronic spontaneous urticaria: induction of histamine release and basophil accumulation by substance P.
Cell Biol Toxicol. 32 (3): 217-28. -
Jin Y et al. (2015) Allergenic response to squid (Todarodes pacificus) tropomyosin Tod p1 structure modifications induced by high hydrostatic pressure.
Food Chem Toxicol. 76: 86-93. -
Wei, J.F. et al. (2009) Induction of mast cell accumulation, histamine release and skin edema by N49 phospholipase A2.
BMC Immunol. 10: 21. -
Tsai, C.T. et al. (2018) Isotype-specific agglutination-PCR (ISAP): A sensitive and multiplex method for measuring allergen-specific IgE.
J Allergy Clin Immunol. 141 (5): 1901-4.e15.
- RRID
- AB_1221599.
- UniProt
- P01854
- Entrez Gene
- IGHE
- GO Terms
- GO:0003823 antigen binding
- GO:0006955 immune response
View more products with IgE specificity
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