Histone H1.3 antibody

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Rabbit anti Histone H1.3

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00783
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human histone H1.3 antibody recognizes histone H1.3, also known as histone H1c, H1-3 and H1s-2. Histones are the major protein component of chromatin, with important roles in gene regulation. Histone H1 binds to linker DNA between nucleosomes, plays a role in higher-order chromatin stabilization, and is also able to fine-tune gene transcription in a locus-specific way (Brockers and Schneider, 2019). Histone variants replace canonical histones when nucleosomes are disrupted, with diverse implications for epigenetic gene regulation. Histone H1.3 is a histone variant and appears to have diverse roles in cancer. This protein has been suggested as a prognostic biomarker for pancreatic ductal adenocarcinoma (Bauden et al. 2017). Histone H1.3 has also been identified as a repressor for the oncogene H19, contributing to the suppression of epithelial ovarian carcinogenesis (Medrzycki et al. 2015).

Product Details

Target Species
Human
Western Blotting
Anti histone H1.3 antibody recognizes a band of approximately 30 kDa in Jurkat cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the N terminal region of human histone H1.3

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P16402
Entrez Gene
HIST1H1D
GO Terms
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0006334 nucleosome assembly
GO:0005634 nucleus
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Histone H1.3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml