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GAPDH antibody

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Anti Human GAPDH (C-Terminal) Antibody thumbnail image 1 Anti Human GAPDH (C-Terminal) Antibody thumbnail image 2 Anti Human GAPDH (C-Terminal) Antibody thumbnail image 3 Anti Human GAPDH (C-Terminal) Antibody thumbnail image 4
Anti Human GAPDH (C-Terminal) Antibody gallery image 1
Western blotting analysis of human liver (A) and tonsil (B) lysate (35μg protein in RIPA buffer) using Goat anti Human GAPDH antibody (AHP1064) at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Anti Human GAPDH (C-Terminal) Antibody gallery image 2
Western blotting analysis of mouse liver (A) and rat brain (B) lysate (35μg protein in RIPA buffer) using Goat anti Human GAPDH antibody (AHP1064) at 0.5μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Anti Human GAPDH (C-Terminal) Antibody gallery image 3
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation using Goat anti Human GAPDH antibody (AHP1064) for 1hr at a concentration of 5μgml-1 followed by Alexa Fluor 488 conjugated secondary antibody at 1μgml-1, showing cytoplasmic staining (green). The nuclear stain is DAPI (blue)
Anti Human GAPDH (C-Terminal) Antibody gallery image 4
Western blotting analysis of lysates of cell line HEK293 (A) and HeLa (B) (35μg protein in RIPA buffer) using Goat anti Human GAPDH antibody (AHP1064) at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

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Goat anti Human GAPDH (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
GAPDH
Region
(C-TERMINAL)

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Product Code Applications Pack Size List Price Your Price Qty
AHP1064
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.1 mg loader
List Price Your Price
loader

Goat anti Human GAPDH antibody recognizes an epitope within the C-terminal (CT) region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.

GAPDH translocates to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death (Dastoor et al. 2001). GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Burke et al. 1996).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Mouse
Pig
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human GAPDH (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
0.5%Bovine Serum Albumin
Immunogen
Peptide sequence C-HQVVSSDFNSDT corresponding to the C-terminal region of GAPDH (NP_002037.2).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000
Immunohistology - Paraffin 1 0.3ug/ml
Western Blotting 0.01 0.03ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue
Human tonsil
Western Blotting
AHP1064 detects a band of approximately 35kDa in human tonsil, mouse spleen and HeLa cell lysates.

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Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
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Description Antigen Retrieval Buffer, pH8.0

References for GAPDH antibody

  1. Wang H et al. (2013) Hypoxic preconditioning alleviates ethanol neurotoxicity: the involvement of autophagy.
    Neurotox Res. 24 (4): 472-7.

Further Reading

  1. Hara, M.R. et al. (2006) GAPDH as a sensor of NO stress.
    Biochim Biophys Acta. 1762 (5): 502-9.
  2. Mazzola, J.L. & Sirover, M.A. (2001) Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimer's disease and in Huntington's disease fibroblasts.
    J Neurochem. 76 (2): 442-9.

Synonyms
GLYCERALDEHYDE-3-PHOSPHATE Dehydrogenase
RRID
AB_808591
UniProt
P04406
Entrez Gene
GAPDH
GO Terms
GO:0005515 protein binding
GO:0016020 membrane
GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
GO:0050821 protein stabilization
GO:0035605 peptidyl-cysteine S-nitrosylase activity
GO:0035606 peptidyl-cysteine S-trans-nitrosylation
GO:0048471 perinuclear region of cytoplasm
GO:0051287 NAD binding
GO:0051402 neuron apoptosis

AHP1064

155638 164741

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