GAPDH antibody

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Anti Human GAPDH (C-Terminal) Antibody thumbnail image 1

Anti Human GAPDH (C-Terminal) Antibody thumbnail image 2

Anti Human GAPDH (C-Terminal) Antibody thumbnail image 3

Anti Human GAPDH (C-Terminal) Antibody thumbnail image 4

Anti Human GAPDH (C-Terminal) Antibody gallery image 1 — Western blotting analysis of lysates of cell line HEK293 (A) and HeLa (B) (35μg protein in RIPA buffer) using Goat anti Human GAPDH antibody (**AHP1064**) at 0.1μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

Anti Human GAPDH (C-Terminal) Antibody gallery image 2 — Western blotting analysis of human liver (A) and tonsil (B) lysate (35μg protein in RIPA buffer) using Goat anti Human GAPDH antibody (**AHP1064**) at 0.1μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Anti Human GAPDH (C-Terminal) Antibody gallery image 3 — Western blotting analysis of mouse liver (A) and rat brain (B) lysate (35μg protein in RIPA buffer) using Goat anti Human GAPDH antibody (**AHP1064**) at 0.5μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Anti Human GAPDH (C-Terminal) Antibody gallery image 4 — Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation using Goat anti Human GAPDH antibody (**AHP1064**) for 1hr at a concentration of 5μgml^-1 followed by Alexa Fluor 488 conjugated secondary antibody at 1μgml^-1, showing cytoplasmic staining (green). The nuclear stain is DAPI (blue)

Goat anti Human GAPDH (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1064	E P* WB			0.1 mg

Goat anti Human GAPDH antibody recognizes an epitope within the C-terminal (CT) region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.

GAPDH translocates to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death (Dastoor et al. 2001). GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Burke et al. 1996).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Dog	Expected from Sequence
Mouse
Pig
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human GAPDH (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02%	Sodium Azide (NaN₃)
0.5%	Bovine Serum Albumin

Immunogen

Peptide sequence C-HQVVSSDFNSDT corresponding to the C-terminal region of GAPDH (NP_002037.2).

Approx. Protein Concentrations

IgG concentration 0.5mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P04406 Related reagents
Entrez Gene: GAPDH Related reagents
GO Terms: GO:0005515 protein binding; GO:0016020 membrane; GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; GO:0005829 cytosol; GO:0005634 nucleus; GO:0006094 gluconeogenesis; GO:0006096 glycolysis; GO:0055114 oxidation-reduction process; GO:0050821 protein stabilization; GO:0035605 peptidyl-cysteine S-nitrosylase activity; GO:0035606 peptidyl-cysteine S-trans-nitrosylation; GO:0048471 perinuclear region of cytoplasm; GO:0051287 NAD binding; GO:0051402 neuron apoptosis
Regulatory: For research purposes only

Applications of GAPDH antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA		1/2000
Immunohistology - Paraffin ¹		0.3ug/ml
Western Blotting	0.01	0.03ug/ml

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Human tonsil
Western Blotting: AHP1064 detects a band of approximately 35kDa in human tonsil, mouse spleen and HeLa cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	1 mg	F
Rabbit anti Goat IgG (Fc):HRP	STAR122P	1 mg	C E WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P

Product Specific References

References for GAPDH antibody

Hara, M.R. et al. (2006) GAPDH as a sensor of NO stress.
Biochim Biophys Acta. 1762 (5): 502-9.
Mazzola, J.L. & Sirover, M.A. (2001) Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimer's disease and in Huntington's disease fibroblasts.
J Neurochem. 76 (2): 442-9.
Wang H et al. (2013) Hypoxic preconditioning alleviates ethanol neurotoxicity: the involvement of autophagy.
Neurotox Res. 24 (4): 472-7.

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