ELF1 antibody

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Goat anti ELF1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP3021
Datasheet
P WB
SDS
0.1 mg

Goat anti ELF1 antibody recognizes ETS-related transcription factor Elf-1, also known as E74-like factor 1 (ELF1).

ELF1 is a small zinc-finger protein conserved in eukaryotes and some archaea (Ehara and Sekine 2018). ELF1 is ubiquitously expressed in human cells and is a member of the ETS family of transcription factors (Budka et al. 2018) and appears to act downstream of the DNA damage response pathway (Cheng et al. 2020). In cancer, ELF1 appears to have oncogenic and tumor suppressive functions depending on context (Budka et al. 2018). ELF1 appears to play an antiviral role, with lower levels of ELF1 reducing host defense against the influenza A virus (Seifert et al. 2019).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human ELF1 were raised by repeated immunizations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence C-AMKQNELLEPNSF, from the C Terminus of the protein sequence according to NP_758961.1; NP_001138825.1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P32519
Entrez Gene
ELF1
GO Terms
GO:0005515 protein binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0016563 transcription activator activity
GO:0043565 sequence-specific DNA binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
Regulatory
For research purposes only

Applications of ELF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 2.5 ug/ml
Western Blotting 0.03 ug/ml 0.1 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Goat anti Human ELF1 antibody detects a band of approximately 70 kDa in cell lysates

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

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