Desmin antibody

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Goat anti Human Desmin (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2116 E P* WB 0.1 mg
Goat anti Human Desmin antibody recognizes an epitope within the C-terminal (CT) region of human Desmin, a type III intermediate filament (IF), localized near the Z line in sacromeres of skeletal, smooth and cardiac muscle tissue.

Desmin plays an essential role in the architecture of muscle cells, connecting the Z-disk to the subsarcolemmal cytoskeleton, and the contractile apparatus to the, mitochondria, cell nucleus and post-synaptic areas of motor endplates. Desmin is an early indicator for muscle tissue in embryogenesis, and a marker for tumours of myogenic origin. Mutations in the Desmin gene, are responsible for the neuromuscular disorder known as desmin-related cardio skeletal myopathy (CSM), characterized by skeletal muscle weakness.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Bovine Expected from Sequence
Mouse
Rat
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human Desmin (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-RDGEVVSEATQQQHE from the C-Terminal region of Desmin (NP_001918.3).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P17661 Related reagents
Entrez Gene
DES Related reagents
Regulatory
For research purposes only

Applications of Desmin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000
Immunohistology - Paraffin 1 3.0 6.0ug/ml
Western Blotting 0.1 0.3ug/ml
  1. 1 This product requires heat-mediated antigen retrieval prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human prostate
Western Blotting
Goat anti Human Desmin detects a band of approximately 55kDa in human skeletal muscle cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P