Desmin antibody

Goat anti Human Desmin (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Desmin
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP2116
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.1 mg
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Search for Batch Specific Datasheets

Goat anti Human Desmin antibody recognizes an epitope within the C-terminal (CT) region of human Desmin, a type III intermediate filament (IF), localized near the Z line in sacromeres of skeletal, smooth and cardiac muscle tissue.

Desmin plays an essential role in the architecture of muscle cells, connecting the Z-disk to the subsarcolemmal cytoskeleton, and the contractile apparatus to the, mitochondria, cell nucleus and post-synaptic areas of motor endplates. Desmin is an early indicator for muscle tissue in embryogenesis, and a marker for tumours of myogenic origin. Mutations in the Desmin gene, are responsible for the neuromuscular disorder known as desmin-related cardio skeletal myopathy (CSM), characterized by skeletal muscle weakness.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Bovine Expected from Sequence
Mouse
Rat
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human Desmin (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-RDGEVVSEATQQQHE from the C-Terminal region of Desmin (NP_001918.3).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000
Immunohistology - Paraffin 1 3.0 6.0ug/ml
Western Blotting 0.1 0.3ug/ml
  1. 1 This product requires heat-mediated antigen retrieval prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human prostate
Western Blotting
Goat anti Human Desmin detects a band of approximately 55kDa in human skeletal muscle cell lysates.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
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loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
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loader
Description Rabbit anti Goat IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0

RRID
AB_2092438
UniProt
P17661
Entrez Gene
DES
View more products with Desmin specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products