Cyclin D1 antibody

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Rabbit anti Cyclin D1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00892
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human cyclin D1 antibody recognizes the G1/S-specific cyclin D1, also known as B-cell CLL/lymphoma 1, CCND1, BCL-1 oncogene and PRAD1 oncogene.

The protein encoded by CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of CCND1 is regulated positively by Rb. Mutations, amplification and overexpression of CCND1, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis (provided by RefSeq, Jul 2008).

Rabbit anti Human cyclin D1 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti cyclin D1 antibody recognizes a band of approximately 36 kDa in MCF7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Synthetic peptide of human cyclin D1

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P24385
Entrez Gene
CCND1
GO Terms
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000307 cyclin-dependent protein kinase holoenzyme complex
GO:0001934 positive regulation of protein phosphorylation
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0042493 response to drug
GO:0019901 protein kinase binding
GO:0031571 mitotic cell cycle G1/S transition DNA damage checkpoint
GO:0045737 positive regulation of cyclin-dependent protein kinase activity
GO:0051301 cell division
GO:0070141 response to UV-A
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Cyclin D1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml