Cyclin D1 antibody

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Anti Cyclin D1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with cyclin D1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 28 second exposure. Arrow points to cyclin D1 (molecular weight 36 kDa)

Rabbit anti Cyclin D1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Cyclin D1

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Rabbit anti Human cyclin D1 antibody recognizes the G1/S-specific cyclin D1, also known as B-cell CLL/lymphoma 1, CCND1, BCL-1 oncogene and PRAD1 oncogene.

The protein encoded by CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of CCND1 is regulated positively by Rb. Mutations, amplification and overexpression of CCND1, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis (provided by RefSeq, Jul 2008).

Rabbit anti Human cyclin D1 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti cyclin D1 antibody recognizes a band of approximately 36 kDa in MCF7 cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
<50% Glycerol
Immunogen: Synthetic peptide of human cyclin D1
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P24385
Entrez Gene: CCND1
GO Terms: GO:0000080 G1 phase of mitotic cell cycle; GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0000307 cyclin-dependent protein kinase holoenzyme complex; GO:0001934 positive regulation of protein phosphorylation; GO:0005829 cytosol; GO:0005654 nucleoplasm; GO:0042493 response to drug; GO:0019901 protein kinase binding; View More GO Terms; GO:0031571 mitotic cell cycle G1/S transition DNA damage checkpoint; GO:0045737 positive regulation of cyclin-dependent protein kinase activity; GO:0051301 cell division; GO:0070141 response to UV-A