Collagen IV antibody

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Goat anti Human Collagen IV

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
134001
Datasheet
C E IB IF
MSDS
0.2 mg loader

Goat anti Human collagen IV antibody recognizes human collagen type IV. It has been cross adsorbed against collagen types I, II, III, V and VI, resulting in <10% cross-reactivity. It may cross-react with collagen type IV from other species.

Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Collagen Type IV forms a two-dimensional reticulum and is a major component of the basal lamina.

Goat anti human collagen IV has been successfully employed for the demonstration of vasculature in developing rat pups using immunofluorescence techniques (Kermorvant-Duchemin et al. 2013).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human collagen IV was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Borate buffered saline
Preservative Stabilisers
None present
Immunogen
Human Collagen Type IV from human placenta
Approx. Protein Concentrations
IgG concentration 0.4 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P02462 Related reagents
P08572 Related reagents
Q01955 Related reagents
P53420 Related reagents
P29400 Related reagents
Q14031 Related reagents
Entrez Gene
COL4A1 Related reagents
COL4A2 Related reagents
COL4A5 Related reagents
COL4A4 Related reagents
COL4A3 Related reagents
COL4A6 Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0005201 extracellular matrix structural constituent
GO:0007411 axon guidance
GO:0048407 platelet-derived growth factor binding
GO:0005515 protein binding
GO:0005587 collagen type IV
GO:0016525 negative regulation of angiogenesis
GO:0030198 extracellular matrix organization
GO:0005605 basal lamina
GO:0032836 glomerular basement membrane development
Regulatory
For research purposes only

Applications of Collagen IV antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/4000
Immunoblotting
Immunofluorescence
Immunohistology - Frozen 1/10 1/40
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Product Specific References

References for Collagen IV antibody

  1. Kermorvant-Duchemin, E. et al. (2013) Neonatal hyperglycemia inhibits angiogenesis and induces inflammation and neuronal degeneration in the retina.
    PLoS One. 8(11): e79545.
  2. Dominguez, E. et al. (2015) Experimental Branch Retinal Vein Occlusion Induces Upstream Pericyte Loss and Vascular Destabilization.
    PLoS One. 10 (7): e0132644.
  3. Rajapaksa, K.S. et al. (2016) Preclinical Safety Profile of a Depleting Antibody against CRTh2 for Asthma: Well Tolerated Despite Unexpected CRTh2 Expression on Vascular Pericytes in the Central Nervous System and Gastric Mucosa.
    Toxicol Sci. 152 (1): 72-84.
  4. Trouillet, A. et al. (2017) Col4a1 mutation generates vascular abnormalities correlated with neuronal damage in a mouse model of HANAC syndrome.
    Neurobiol Dis. 100: 52-61.