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Chromogranin A antibody

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Staining of paraffin embedded human pancreas with Sheep anti Human Chromogranin A (2095-0220) following pronase antigen retrieval.

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WB

Sheep anti Human Chromogranin A

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Chromogranin A

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Product Code Applications Pack Size List Price Your Price Qty
2095-0220
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * 1 ml
List Price Your Price

Sheep anti Human Chromogranin antibodyrecognizes human chromogranin A, a secreted peptide that inhibits glucose-induced insulin release. Chromogranin A is an activator of microglia (Taupenaut et al. 1996) and is notably upregulated and prominantly expressed in the senile plaques associated with Alzheimer's disease (Yasuhara et al. 1994).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
Chimpanzee Expected from Sequence
Dog Expected from Sequence
Horse Expected from Sequence
Pig Expected from Sequence
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the amino acids 1 - 15 (lpvnspmnkgdtevm) of the native molecule conjugated to Keyhole limpet Haemocyanin.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1 1/25 1/100
  1. 1This product requires protein digestion pre-treatment of paraffin sections. Pronase is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human pancreas

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
List Price Your Price
loader
Description Rabbit anti Sheep IgG (H/L):Biotin

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0

RRID
AB_2260642
UniProt
P10645
Entrez Gene
CHGA
GO Terms
GO:0005576 extracellular region
GO:0008217 regulation of blood pressure

2095-0220

0515 151002

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