CDK10 antibody

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Goat anti CDK10

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP3009
Datasheet
WB
SDS
0.1 mg

Goat anti Human CDK10 antibody recognizes cyclin-dependent kinase 10 (CDK10), also known as serine/threonine-protein kinase PISSLRE.

Cyclin-dependent kinases (CDKs) are serine/threonine kinases with activity regulated by specific cyclin partners, and by CDK inhibitors (CKIs). CDKs are well-known for controlling progression through the cell cycle, but also regulate many other cellular processes (Lim and Kaldis 2013). Cyclin M interacts with CDK10, and the CDK10/Cyclin M complex mediates degradation of the oncoprotein and transcription factor ETS2 (Guen et al.. 2013). Loss of CDK10 appears to be a major determinant of resistence to endocrine therapy for breast cancer (Iorns et al.. 2008). In addition, CDK10 seems to act as a tumor suppressor in nasopharyngeal carcicoma and in gastric cancer (You et al.. 2018). Using a zebrafish model, CDK10 was found to participate in transcriptional complexes that play key roles during development (Guen et al.. 2017).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CDK10 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence AEPDLECEQIRLK , from the N Terminus of the protein sequence according to NP_443714.3.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CDK10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 3 µg/ml 0.5 µg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Goat anti Human CDK10 antibody detects a band of approximately 38 kDa in cell lysates.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

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