Goat anti Human CDK10 antibody
recognizes cyclin-dependent kinase 10 (CDK10), also known as serine/threonine-protein kinase PISSLRE.
Cyclin-dependent kinases (CDKs) are serine/threonine kinases with activity regulated by specific cyclin partners, and by CDK inhibitors (CKIs). CDKs are well-known for controlling progression through the cell cycle, but also regulate many other cellular processes (Lim and Kaldis 2013
). Cyclin M interacts with CDK10, and the CDK10/Cyclin M complex mediates degradation of the oncoprotein and transcription factor ETS2 (Guen et al. 2013
). Loss of CDK10 appears to be a major determinant of resistence to endocrine therapy for breast cancer (Iorns et al. 2008
). In addition, CDK10 seems to act as a tumor suppressor in nasopharyngeal carcicoma and in gastric cancer (You et al. 2018
). Using a zebrafish model, CDK10 was found to participate in transcriptional complexes that play key roles during development (Guen et al. 2017
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human CDK10 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
0.5% Bovine Serum Albumin
- Peptide with sequence AEPDLECEQIRLK , from the N Terminus of the protein sequence according to NP_443714.3.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CDK10 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Goat anti Human CDK10 antibody detects a band of approximately 38 kDa in cell lysates.
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Secondary Antibodies Available
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