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CD194 antibody

Goat anti Human CD194

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD194

Product Code Applications Pack Size List Price Your Price Qty
AHP1809
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F P WB 0.1 mg loader
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loader

Goat anti Human CD194 antibody recognises human CD194, otherwise known as CCR4 or C-C chemokine receptor type 4. CD194 is a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, preferentially expressed by CD4+ Th2 (type 2 helper T) cells, which acts as a high affinity receptor for the chemokines MDC (macrophage-derived chemokine), MCP-1, MIP-1, RANTES and TARC.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human CD194 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Immunogen
Synthetic peptide SNYYLYESIPKPCTKEGIKAFGE corresponding to amino acids 17-39 of human CD194.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/30,000
Flow Cytometry Neat
Immunohistology - Frozen
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/100 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Spleen

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Synonyms
CCR4
RRID
AB_2074495
UniProt
P51679
Entrez Gene
CCR4
GO Terms
GO:0005887 integral to plasma membrane
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007204 elevation of cytosolic calcium ion concentration

AHP1809

161110 163811

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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