Cathepsin B antibody

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Goat anti Cathepsin B

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Cathepsin B

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AHP3008 Datasheet SDS SDS	F IF P WB	0.1 mg		Log in
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Anti Cathepsin B Antibody thumbnail image 1

Anti Cathepsin B Antibody thumbnail image 2

Anti Cathepsin B Antibody thumbnail image 3

Anti Cathepsin B Antibody thumbnail image 4

Anti Cathepsin B Antibody gallery image 1 — Western blot analysis of A) human lung, B) HCORC, and C) HCBGLC cell lysates (35 µg protein in RIPA buffer) probed with Goat anti cathepsin B antibody (1 µg/ml for human lung and HCORC, 0.5 µg/ml for HCBGLC, **AHP3008**) and detected by chemiluminescence

Anti Cathepsin B Antibody gallery image 2 — Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% octylphenol ethoxylate. Primary incubation 1 hr with Goat anti cathepsin B antibody, 10 &mug/ml,(**AHP3008**) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized Goat IgG (10 μg/ml) followed by Alexa Fluor 488 conjugated secondary antibody (2 μg/ml).

Anti Cathepsin B Antibody gallery image 3 — Flow cytometry analysis of paraformaldehyde fixed, 0.5% octylphenol ethoxylate permeablised HeLa cells incubated with Goat anti cathepsin B antibody (10 μg/ml, **AHP3008**) followed by Alexa Fluor 488 secondary antibody. IgG control (black line): unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody.

Anti Cathepsin B Antibody gallery image 4 — Western blot analysis of HepG2 cell lysate (35 μg protein in RIPA buffer) probed with Goat anti cathepsin B antibody (1 μg/ml, **AHP3008**) and detected by chemiluminescence.

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Goat anti Human cathepsin B antibody recognizes cathepsin B, also known as APPS and CPSB.

Cathepsin B is a lysosomal cysteine protease, expressed constituitively and linked to protein turnover in lysosomes (Aggarwal and Sloane 2014).

Target Species: Human
Product Form: Purified IgG - liquid
Antiserum Preparation: Antisera to human cathepsin B were raised by repeated immunizations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution: TRIS buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
0.5% Bovine Serum Albumin
Immunogen: Peptide with sequence SDRICIHTNAHVSVE, from the internal region of the protein sequence according to NP_001899.1
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature.
Store at -20°C only.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	10 μg/ml
Immunofluorescence	10 µg/ml
Immunohistology - Paraffin	5 µg/ml
Western Blotting	3 µg/ml	0.5 µg/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Goat anti Human cathepsin B antibody detects a band of approximately 35 kDa in cell lysates

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