Rabbit anti Human C4d antibody is specific for human complement split product C4d.
- Target Species
- Product Form
- Purified Ig - liquid
- Purified Ig prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Proclin™300
- Synthetic peptide corresponding to amino acids 1241-1256 of C4 conjugated to Keyhole limpet Haemocyanin.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- Guaranteed until date of expiry. Please see product label.
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of C4d antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. TRIS/EDTA buffer pH 8.5 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for C4d antibody
Titipungul, T. et al. (2012) Percentage of C4d staining in lupus nephritis and correlation between C4d staining with serum C3, serum C4 level and other parameter in lupus nephritis.
Mahasarakham Hospital Journal 9: 27-34
Regele, H. et al. (2001) Endothelial C4d deposition is associated with inferior kidney allograft outcome independently of cellular rejection.
Nephrol Dial Transplant. 16 (10): 2058-66.
Santos, A. et al. (2012) C4d detection in renal allograft biopsies: immunohistochemistry vs. immunofluorescence
Port J Nephrol Hypert 26 (4): 272-7.
Sethi, S. et al. (2015) C4d as a Diagnostic Tool in Proliferative GN.
J Am Soc Nephrol. 26 (11): 2852-9.
Viana, H. et al. (2009) C4d presence in kidney allograft biopsy: sensitivity and specificity of immunoperoxidase vs. immunofluorescence.
N: 10th Banff Conference on Allograft Pathology, 2009, 9 a 14 Ago. Banff, Alberta, Canada
Sreedharanunni, S. et al. (2014) An analysis of transplant glomerulopathy and thrombotic microangiopathy in kidney transplant biopsies.
Transpl Int. 27 (8): 784-92.
Hanaoka,K. et al. (2019) Benefits of a loading dose of tacrolimus on graft survival of kidney transplants in nonhuman primates
Transplant Immunology. 52: 32-9.
Mahakur, S. et al. (2018) Allo-specific immune response profiles indicative of acute rejection in kidney allografts using an in vitro lymphocyte culture-based model.
Clin Exp Nephrol. 22 (2): 465-73.
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