C4d antibody

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Anti Human C4d Antibody thumbnail image 2

Anti Human C4d Antibody thumbnail image 3

Anti Human C4d Antibody gallery image 1 — Immunohistochemical staining of human kidney with Rabbit anti Human C4d antibody (**0300-0230**) showing localization to the peritubular capillaries

Anti Human C4d Antibody gallery image 2 — Formalin fixed, paraffin embedded human kidney from a patient with lupus erythematosus stained with Rabbit anti Human C4d antibody (**0300-0230**) following Sodium citrate (pH6.0) mediated antigen retrieval: low power

Anti Human C4d Antibody gallery image 3 — Formalin fixed paraffin embedded human kidney for a patient with Lupus erythematosus stained with Rabbit anti Human C4d antibody (**0300-0230**) following Sodium citrate (pH6.0) mediated antigen retrieval: high power

Rabbit anti Human C4d

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
0300-0230 Datasheet	C IF P* SDS	0.25 ml	Log in		Get Quote

Rabbit anti Human C4d antibody is specific for human complement split product C4d.

Product Details

Target Species: Human
Product Form: Purified Ig - liquid
Preparation: Purified Ig prepared by affinity chromatography on Protein G
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.1% Proclin™300
Immunogen: Synthetic peptide corresponding to amino acids 1241-1256 of C4 conjugated to Keyhole limpet Haemocyanin.

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: Guaranteed until date of expiry. Please see product label.

More Information

UniProt: P0C0L4; P0C0L5
Entrez Gene: C4A; C4B
Regulatory: For research purposes only

Applications of C4d antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunofluorescence	1/20	1/80
Immunohistology - Frozen	1/20	1/80
Immunohistology - Paraffin ¹	1/20	1/80

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. TRIS/EDTA buffer pH 8.5 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in		Get Quote
100x Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in		Get Quote

Application Based External Images

Immunofluorescence

Product Specific References

References for C4d antibody

Titipungul, T. et al. (2012) Percentage of C4d staining in lupus nephritis and correlation between C4d staining with serum C3, serum C4 level and other parameter in lupus nephritis.
Mahasarakham Hospital Journal 9: 27-34
Regele, H. et al. (2001) Endothelial C4d deposition is associated with inferior kidney allograft outcome independently of cellular rejection.
Nephrol Dial Transplant. 16 (10): 2058-66.
Santos, A. et al. (2012) C4d detection in renal allograft biopsies: immunohistochemistry vs. immunofluorescence
Port J Nephrol Hypert 26 (4): 272-7.
Sethi, S. et al. (2015) C4d as a Diagnostic Tool in Proliferative GN.
J Am Soc Nephrol. 26 (11): 2852-9.
Viana, H. et al. (2009) C4d presence in kidney allograft biopsy: sensitivity and specificity of immunoperoxidase vs. immunofluorescence.
N: 10th Banff Conference on Allograft Pathology, 2009, 9 a 14 Ago. Banff, Alberta, Canada
Sreedharanunni, S. et al. (2014) An analysis of transplant glomerulopathy and thrombotic microangiopathy in kidney transplant biopsies.
Transpl Int. 27 (8): 784-92.
Hanaoka,K. et al. (2019) Benefits of a loading dose of tacrolimus on graft survival of kidney transplants in nonhuman primates
Transplant Immunology. 52: 32-9.
Mahakur, S. et al. (2018) Allo-specific immune response profiles indicative of acute rejection in kidney allografts using an in vitro lymphocyte culture-based model.
Clin Exp Nephrol. 22 (2): 465-73.

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