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C4d antibody

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Anti Human C4d Antibody thumbnail image 1 Anti Human C4d Antibody thumbnail image 2 Anti Human C4d Antibody thumbnail image 3
Anti Human C4d Antibody gallery image 1
Immunohistochemical staining of human kidney with Rabbit anti Human C4d antibody (0300-0230) showing localization to the peritubular capillaries
Anti Human C4d Antibody gallery image 2
Formalin fixed, paraffin embedded human kidney from a patient with lupus erythematosus stained with Rabbit anti Human C4d antibody (0300-0230) following Sodium citrate (pH6.0) mediated antigen retrieval: low power
Anti Human C4d Antibody gallery image 3
Formalin fixed paraffin embedded human kidney for a patient with Lupus erythematosus stained with Rabbit anti Human C4d antibody (0300-0230) following Sodium citrate (pH6.0) mediated antigen retrieval: high power

Rabbit anti Human C4d

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
0300-0230
Datasheet
C IF P*
SDS
0.25 ml loader

Rabbit anti Human C4d antibody is specific for human complement split product C4d.

Product Details

Target Species
Human
Product Form
Purified Ig - liquid
Preparation
Purified Ig prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Proclin™300
Immunogen
Synthetic peptide corresponding to amino acids 1241-1256 of C4 conjugated to Keyhole limpet Haemocyanin.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P0C0L4
P0C0L5
Entrez Gene
C4A
C4B
Regulatory
For research purposes only

Applications of C4d antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/20 1/80
Immunohistology - Frozen 1/20 1/80
Immunohistology - Paraffin 1 1/20 1/80
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. TRIS/EDTA buffer pH 8.5 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Application Based External Images

Immunofluorescence

Product Specific References

References for C4d antibody

  1. Regele, H. et al. (2001) Endothelial C4d deposition is associated with inferior kidney allograft outcome independently of cellular rejection.
    Nephrol Dial Transplant. 16 (10): 2058-66.
  2. Santos, A. et al. (2012) C4d detection in renal allograft biopsies: immunohistochemistry vs. immunofluorescence
    Port J Nephrol Hypert 26 (4): 272-7.
  3. Sethi, S. et al. (2015) C4d as a Diagnostic Tool in Proliferative GN.
    J Am Soc Nephrol. 26 (11): 2852-9.
  4. Viana, H. et al. (2009) C4d presence in kidney allograft biopsy: sensitivity and specificity of immunoperoxidase vs. immunofluorescence.
    N: 10th Banff Conference on Allograft Pathology, 2009, 9 a 14 Ago. Banff, Alberta, Canada
  5. Sreedharanunni, S. et al. (2014) An analysis of transplant glomerulopathy and thrombotic microangiopathy in kidney transplant biopsies.
    Transpl Int. 27 (8): 784-92.
  6. Hanaoka,K. et al. (2019) Benefits of a loading dose of tacrolimus on graft survival of kidney transplants in nonhuman primates
    Transplant Immunology. 52: 32-9.
  7. Mahakur, S. et al. (2018) Allo-specific immune response profiles indicative of acute rejection in kidney allografts using an in vitro lymphocyte culture-based model.
    Clin Exp Nephrol. 22 (2): 465-73.

Fluorescent Spectraviewer

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