C4d antibody

Product Code Applications Pack Size List Price Your Price Qty
0300-0230
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SDS Safety Datasheet SDS
C IF P * 0.25 ml loader
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Rabbit anti Human C4d antibody is specific for human complement split product C4d.

Target Species
Human
Product Form
Purified Ig - liquid
Preparation
Purified Ig prepared by affinity chromatography on Protein G.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.1% Proclin 950
Immunogen
Synthetic peptide corresponding to amino acids 1241-1256 of C4 conjugated to Keyhole limpet Haemocyanin.
Regulatory
For research purposes only.
Guarantee
Guaranteed until date of expiry. Please see product label.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/20 1/80
Immunohistology - Frozen 1/20 1/80
Immunohistology - Paraffin 1 1/20 1/80
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. TRIS/EDTA buffer pH 8.5 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for C4d antibody

  1. Regele, H. et al. (2001) Endothelial C4d deposition is associated with inferior kidney allograft outcome independently of cellular rejection.
    Nephrol Dial Transplant. 16 (10): 2058-66.
  2. Santos, A. et al. (2012) C4d detection in renal allograft biopsies: immunohistochemistry vs. immunofluorescence
    Port J Nephrol Hypert 26 (4): 272-7.
  3. Sethi, S. et al. (2015) C4d as a Diagnostic Tool in Proliferative GN.
    J Am Soc Nephrol. 26 (11): 2852-9.
  4. Viana, H. et al. (2009) C4d presence in kidney allograft biopsy: sensitivity and specificity of immunoperoxidase vs. immunofluorescence.
    N: 10th Banff Conference on Allograft Pathology, 2009, 9 a 14 Ago. Banff, Alberta, Canada
  5. Sreedharanunni, S. et al. (2014) An analysis of transplant glomerulopathy and thrombotic microangiopathy in kidney transplant biopsies.
    Transpl Int. 27 (8): 784-92.
  6. Hanaoka,K. et al. (2019) Benefits of a loading dose of tacrolimus on graft survival of kidney transplants in nonhuman primates
    Transplant Immunology. 52: 32-9.
  7. Mahakur, S. et al. (2018) Allo-specific immune response profiles indicative of acute rejection in kidney allografts using an in vitro lymphocyte culture-based model.
    Clin Exp Nephrol. 22 (2): 465-73.

Immunofluorescence

Synonyms
Complement Component 4d
RRID
AB_620313
UniProt
P0C0L4
P0C0L5
Entrez Gene
C4A
C4B
View more products with C4D specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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