C4d antibody
Rabbit anti Human C4d antibody is specific for human complement split product C4d.
Product Details
- Target Species
- Human
- Product Form
- Purified Ig - liquid
- Preparation
- Purified Ig prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Proclin™300
- Immunogen
- Synthetic peptide corresponding to amino acids 1241-1256 of C4 conjugated to Keyhole limpet Haemocyanin.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P0C0L4 Related reagents
- P0C0L5 Related reagents
- Entrez Gene
- C4A Related reagents
- C4B Related reagents
- Regulatory
- For research purposes only
Applications of C4d antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | 1/20 | 1/80 | |
| Immunohistology - Frozen | 1/20 | 1/80 | |
| Immunohistology - Paraffin 1 | 1/20 | 1/80 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. TRIS/EDTA buffer pH 8.5 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P |
Product Specific References
References for C4d antibody
-
Regele, H. et al. (2001) Endothelial C4d deposition is associated with inferior kidney allograft outcome independently of cellular rejection.
Nephrol Dial Transplant. 16 (10): 2058-66. -
Santos, A. et al. (2012) C4d detection in renal allograft biopsies: immunohistochemistry vs. immunofluorescence
Port J Nephrol Hypert 26 (4): 272-277. -
Titipungul, T. et al. (2012) Percentage of C4d staining in lupus nephritis and correlation between C4d staining with serum C3, serum C4 level and other parameter in lupus nephritis.
Mahasarakham Hospital Journal 9: 27-34 -
Viana, H. et al. (2009) C4d presence in kidney allograft biopsy: sensitivity and specificity of immunoperoxidase vs. immunofluorescence.
N: 10th Banff Conference on Allograft Pathology, 2009, 9 a 14 Ago. Banff, Alberta, Canada -
Sreedharanunni, S. et al. (2014) An analysis of transplant glomerulopathy and thrombotic microangiopathy in kidney transplant biopsies.
Transpl Int. 27 (8): 784-92. -
Sethi, S. et al. (2015) C4d as a Diagnostic Tool in Proliferative GN.
J Am Soc Nephrol. 26 (11): 2852-9.
