C4d antibody

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Rabbit anti Human C4d

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0300-0230 C IF P* 0.25 ml
Rabbit anti Human C4d antibody is specific for human complement split product C4d.

Product Details

Target Species
Human
Product Form
Purified Ig - liquid
Preparation
Purified Ig prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 1241-1256 of C4 conjugated to Keyhole limpet Haemocyanin.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P0C0L4 Related reagents
P0C0L5 Related reagents
Entrez Gene
C4A Related reagents
C4B Related reagents
Regulatory
For research purposes only

Applications of C4d antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/20 1/80
Immunohistology - Frozen 1/20 1/80
Immunohistology - Paraffin 1 1/20 1/80
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. TRIS/EDTA buffer pH 8.5 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Application Based External Images

Immunofluorescence

Product Specific References

References for C4d antibody

  1. Regele, H. et al. (2001) Endothelial C4d deposition is associated with inferior kidney allograft outcome independently of cellular rejection.
    Nephrol Dial Transplant. 16 (10): 2058-66.
  2. Santos, A. et al. (2012) C4d detection in renal allograft biopsies: immunohistochemistry vs. immunofluorescence
    Port J Nephrol Hypert 26 (4): 272-277.
  3. Titipungul, T. et al. (2012) Percentage of C4d staining in lupus nephritis and correlation between C4d staining with serum C3, serum C4 level and other parameter in lupus nephritis.
    Mahasarakham Hospital Journal 9: 27-34
  4. Viana, H. et al. (2009) C4d presence in kidney allograft biopsy: sensitivity and specificity of immunoperoxidase vs. immunofluorescence.
    N: 10th Banff Conference on Allograft Pathology, 2009, 9 a 14 Ago. Banff, Alberta, Canada
  5. Sreedharanunni, S. et al. (2014) An analysis of transplant glomerulopathy and thrombotic microangiopathy in kidney transplant biopsies.
    Transpl Int. 27 (8): 784-92.
  6. Sethi, S. et al. (2015) C4d as a Diagnostic Tool in Proliferative GN.
    J Am Soc Nephrol. 26 (11): 2852-9.