Aurora-A Kinase antibody

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Rabbit anti Aurora-A Kinase

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00851
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human aurora-A kinase antibody recognizes the aurora-A kinase, also known as ARK-1, IPL1-related kinase, aurora 2 and serine/threonine-protein kinase aurora-A.

The protein encoded by AURKA is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. AURKA may play a role in tumor development and progression. A processed pseudogene of AURKA has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for AURKA (provided by RefSeq, Jul 2008).

Rabbit anti Human aurora-A kinase antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti aurora-A kinase antibody recognizes a band of approximately 48 kDa in NIH/3T3 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen
Recombinant aurora-A kinase protein (amino acids 55-250)

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
O14965
Entrez Gene
AURKA
GO Terms
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005876 spindle microtubule
GO:0006468 protein phosphorylation
GO:0007051 spindle organization
GO:0007067 mitosis
GO:0019901 protein kinase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0031616 spindle pole centrosome
GO:0031647 regulation of protein stability
GO:0048471 perinuclear region of cytoplasm
GO:0051301 cell division
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Aurora-A Kinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml