Aurora-A Kinase antibody
Rabbit anti Aurora-A Kinase
Aurora-A kinase antibody recognizes aurora-A kinase, also known as ARK-1, IPL1-related kinase, aurora 2, or serine/threonine-protein kinase aurora-A.
Aurora-A Kinase Antibody with Control Lysate
Aurora-A kinase antibody recognizes aurora-A kinase, also known as ARK-1, IPL1-related kinase, aurora 2, or serine/threonine-protein kinase aurora-A.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00851 | WB | 100 µl | ||||
| VPA00851KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human aurora-A kinase antibody recognizes the aurora-A kinase, also known as ARK-1, IPL1-related kinase, aurora 2 and serine/threonine-protein kinase aurora-A.
The protein encoded by AURKA is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. AURKA may play a role in tumor development and progression. A processed pseudogene of AURKA has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for AURKA (provided by RefSeq, Jul 2008).
Rabbit anti Human aurora-A kinase antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by AURKA is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. AURKA may play a role in tumor development and progression. A processed pseudogene of AURKA has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for AURKA (provided by RefSeq, Jul 2008).
Rabbit anti Human aurora-A kinase antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti aurora-A kinase antibody recognizes a band of approximately 48 kDa in NIH/3T3 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on Protein A
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin - Immunogen
- Recombinant aurora-A kinase protein (amino acids 55-250)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg NIH/3T3 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- O14965 Related reagents
- Entrez Gene
- AURKA Related reagents
- GO Terms
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005876 spindle microtubule
- GO:0006468 protein phosphorylation
- GO:0007051 spindle organization
- GO:0007067 mitosis
- GO:0019901 protein kinase binding
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0031616 spindle pole centrosome
- GO:0031647 regulation of protein stability
- GO:0048471 perinuclear region of cytoplasm
- GO:0051301 cell division
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Aurora-A Kinase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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