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Apolipoprotein E antibody

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Western Blot Validated
Anti Apolipoprotein E Antibody (PrecisionAb™ Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with apolipoprotein E antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 5 second exposure. Arrow points to apolipoprotein E (molecular weight 35 kDa)

Rabbit anti Apolipoprotein E

Apolipoprotein E Antibody with Control Lysate

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00665 WB 100 µl
VPA00665KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human apolipoprotein E antibody recognizes apolipoprotein E, also known as apolipoprotein E3, APOE, APO-E, or LPG.

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants (provided by RefSeq, Jul 2008).

Rabbit anti Human apolipoprotein E antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti apolipoprotein E detects a band of approximately 35 kDa in HepG2 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
1% Bovine Serum Albumin
<50% Glycerol
Preservative Stabilisers
0.01% Thiomersal
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
Carrier-protein conjugated synthetic peptide encompassing part of the C terminal region of human apolipoprotein E
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HepG2 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P02649 Related reagents
Entrez Gene
APOE Related reagents
GO Terms
GO:0000302 response to reactive oxygen species
GO:0001540 beta-amyloid binding
GO:0001937 negative regulation of endothelial cell proliferation
GO:0007010 cytoskeleton organization
GO:0005543 phospholipid binding
GO:0006641 triglyceride metabolic process
GO:0006898 receptor-mediated endocytosis
GO:0006916 anti-apoptosis
GO:0006917 induction of apoptosis
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0007263 nitric oxide mediated signal transduction
GO:0007271 synaptic transmission, cholinergic
GO:0008201 heparin binding
GO:0008219 cell death
GO:0010544 negative regulation of platelet activation
GO:0010873 positive regulation of cholesterol esterification
GO:0010875 positive regulation of cholesterol efflux
GO:0016209 antioxidant activity
GO:0019934 cGMP-mediated signaling
GO:0030425 dendrite
GO:0030516 regulation of axon extension
GO:0030828 positive regulation of cGMP biosynthetic process
GO:0032488 Cdc42 protein signal transduction
GO:0032805 positive regulation of low-density lipoprotein particle receptor catabolic process
GO:0033344 cholesterol efflux
GO:0033700 phospholipid efflux
GO:0034361 very-low-density lipoprotein particle
GO:0034362 low-density lipoprotein particle
GO:0034363 intermediate-density lipoprotein particle
GO:0034364 high-density lipoprotein particle
GO:0034372 very-low-density lipoprotein particle remodeling
GO:0034375 high-density lipoprotein particle remodeling
GO:0034380 high-density lipoprotein particle assembly
GO:0034382 chylomicron remnant clearance
GO:0034384 high-density lipoprotein particle clearance
GO:0034447 very-low-density lipoprotein particle clearance
GO:0046911 metal chelating activity
GO:0042627 chylomicron
GO:0042632 cholesterol homeostasis
GO:0042803 protein homodimerization activity
GO:0043025 neuronal cell body
GO:0043407 negative regulation of MAP kinase activity
GO:0043537 negative regulation of blood vessel endothelial cell migration
GO:0043691 reverse cholesterol transport
GO:0045541 negative regulation of cholesterol biosynthetic process
GO:0046907 intracellular transport
GO:0046982 protein heterodimerization activity
GO:0048156 tau protein binding
GO:0048168 regulation of neuronal synaptic plasticity
GO:0050728 negative regulation of inflammatory response
GO:0070326 very-low-density lipoprotein particle receptor binding
GO:0050750 low-density lipoprotein particle receptor binding
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0060228 phosphatidylcholine-sterol O-acyltransferase activator activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Apolipoprotein E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
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