Androgen Receptor antibody

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Goat anti Androgen Receptor

Androgen receptor antibody recognizes androgen receptor, also known as dihydrotestosterone receptor or nuclear receptor subfamily 3 group C member 4. The antibody has been extensively validated for western blotting using whole cell lysates.

Androgen Receptor Antibody with Control Lysate

Androgen receptor antibody recognizes androgen receptor, also known as dihydrotestosterone receptor or nuclear receptor subfamily 3 group C member 4. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00046 WB 100 µl
VPA00046KT WB 2 Westerns
With Lysate 400µg
Goat anti Human androgen receptor antibody recognizes the human androgen receptor (AR), also known as the dihydrotestosterone receptor or nuclear receptor subfamily 3 group C member 4. AR is a ligand-activated transcription factor that belongs to a large family of nuclear receptor proteins. Unbound AR resides predominately in the cytoplasm as a heteromeric complex with hsp90 and other chaperone proteins. These chaperone proteins maintain AR in a form that is receptive to ligand binding. Upon ligand binding, AR recruits a variety of factors including coactivators and mediator proteins whose enzymatic activities promote chromatin remodeling and transcriptional regulation of target genes leading to cell differentiation, survival, and proliferation.

Mutated AR is strongly implicated in development of prostate adenocarcinoma and metastases development.

Goat anti Human androgen receptor antibody recognizes androgen receptor as a single band of approximately 110 kDa in LNCaP cell lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Goat anti androgen receptor detects a band of approximately 110 kDa in LNCaP cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence EVQLGLGRVYPRPPSC from the N-terminus of the androgen receptor.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg LNCaP lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P10275 Related reagents
Entrez Gene
AR Related reagents
GO Terms
GO:0016049 cell growth
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005102 receptor binding
GO:0004882 androgen receptor activity
GO:0005497 androgen binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0006810 transport
GO:0007267 cell-cell signaling
GO:0008013 beta-catenin binding
GO:0008134 transcription factor binding
GO:0008219 cell death
GO:0008270 zinc ion binding
GO:0008283 cell proliferation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0019899 enzyme binding
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0046983 protein dimerization activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Androgen Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

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