AMPK Alpha 2 antibody
AMPK has been implicated as a suppressor of cell proliferation of both normal and malignant cells. AMPK binding to the PS domain of ULK1, is essential for ULK1-mediated autophagy (Roach et al. 2011), and that the phosphorylation of raptor by AMPK, plays an important role in autophagy induction (Egan et al. 2011).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Dog Expected from Sequence Bovine Expected from Sequence Mouse Rat Pig Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human AMPK alpha 2 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
- Synthetic peptide sequence CPLDALNTTKP from the internal region of AMPK alpha 2 (NP_006243.2).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- P54646 Related reagents
- Entrez Gene
- PRKAA2 Related reagents
- GO Terms
- GO:0005829 cytosol
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006112 energy reserve metabolic process
- GO:0006468 protein phosphorylation
- GO:0006633 fatty acid biosynthetic process
- GO:0006695 cholesterol biosynthetic process
- GO:0006853 carnitine shuttle
- GO:0007050 cell cycle arrest
- GO:0008286 insulin receptor signaling pathway
- GO:0042304 regulation of fatty acid biosynthetic process
- GO:0046320 regulation of fatty acid oxidation
- For research purposes only
Applications of AMPK Alpha 2 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Western Blotting||1.0||3.0 ug/ml|
- Western Blotting
- AHP2256 detects a band of approximately 65kDa in Human heart and liver cell lysates.