AMPK Alpha 2 antibody
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WB E ResetGoat anti Human AMPK Alpha 2
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- AMPK Alpha 2
Antibody Quality and Performance Guarantee
| Goat anti Human AMPK alpha 2 antibody recognizes human5′-AMP-activated protein kinase catalytic subunit alpha-2, also known as AMPK alpha 2, ACACA kinase, Acetyl-CoA carboxylase kinase or Hydroxymethylglutaryl-CoA reductase kinase, encoded by the PRKAA2 gene. AMPK alpha 2 is a a Serine/threonine-protein kinase and the catalytic subunit of the AMP-activated protein kinase (AMPK), a central metabolic sensor, essential for the regulation of fatty acid and cholesterol synthesis, in response to fluctuations in nutrient and intracellular energy levels. AMPK has been implicated as a suppressor of cell proliferation of both normal and malignant cells. AMPK binding to the PS domain of ULK1, is essential for ULK1-mediated autophagy (Roach et al. 2011), and that the phosphorylation of raptor by AMPK, plays an important role in autophagy induction (Egan et al. 2011). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Expected from Sequence Bovine Expected from Sequence Mouse Rat Pig Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human AMPK alpha 2 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence CPLDALNTTKP from the internal region of AMPK alpha 2 (NP_006243.2).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/16000 | |
| Western Blotting | |
1.0 | 3.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP2256 detects a band of approximately 65kDa in Human heart and liver cell lysates.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
- Synonyms
- PRKAA2
- RRID
- AB_10896338
- UniProt
- P54646
- Entrez Gene
- PRKAA2
- GO Terms
- GO:0005829 cytosol
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006112 energy reserve metabolic process
- GO:0006468 protein phosphorylation
- GO:0006633 fatty acid biosynthetic process
- GO:0006695 cholesterol biosynthetic process
- GO:0006853 carnitine shuttle
- View More GO Terms
- GO:0007050 cell cycle arrest
- GO:0008286 insulin receptor signaling pathway
- GO:0042304 regulation of fatty acid biosynthetic process
- GO:0046320 regulation of fatty acid oxidation
View more products with AMPK specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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