AMPK Alpha 2 antibody



AMPK has been implicated as a suppressor of cell proliferation of both normal and malignant cells. AMPK binding to the PS domain of ULK1, is essential for ULK1-mediated autophagy (Roach et al. 2011), and that the phosphorylation of raptor by AMPK, plays an important role in autophagy induction (Egan et al. 2011).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Expected from Sequence Bovine Expected from Sequence Mouse Rat Pig Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human AMPK alpha 2 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence CPLDALNTTKP from the internal region of AMPK alpha 2 (NP_006243.2).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P54646
- Entrez Gene
- PRKAA2
- GO Terms
- GO:0005829 cytosol
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006112 energy reserve metabolic process
- GO:0006468 protein phosphorylation
- GO:0006633 fatty acid biosynthetic process
- GO:0006695 cholesterol biosynthetic process
- GO:0006853 carnitine shuttle
- GO:0007050 cell cycle arrest
- GO:0008286 insulin receptor signaling pathway
- GO:0042304 regulation of fatty acid biosynthetic process
- GO:0046320 regulation of fatty acid oxidation
- Regulatory
- For research purposes only
Applications of AMPK Alpha 2 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/16000 | ||
Western Blotting | 1.0 | 3.0 ug/ml |
- Western Blotting
- AHP2256 detects a band of approximately 65kDa in Human heart and liver cell lysates.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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