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AMPK Alpha 2 antibody

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Anti Human AMPK Alpha 2 Antibody thumbnail image 1 Anti Human AMPK Alpha 2 Antibody thumbnail image 2 Anti Human AMPK Alpha 2 Antibody thumbnail image 3
Anti Human AMPK Alpha 2 Antibody gallery image 1
Western blotting analysis of human liver lysate (35µg protein in RIPA buffer) probed with Goat anti Human AMPK alpha antibody (AHP2256) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Anti Human AMPK Alpha 2 Antibody gallery image 2
Western blotting analysis of mouse (A) and rat (B) heart lysate (35μg protein in RIPA buffer) probed with Goat anti HUman AMPK alpha antibody (AHP2256) at 0.3μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Anti Human AMPK Alpha 2 Antibody gallery image 3
ELISA assay for the evaluation of AMPK alpha levels using Goat anti Human AMPK alpha antibody (AHP2256) as the detection reagent at 1.5μgml-1 with a rabbit polyclonal anti AMPK alpha as the capture reagent

Goat anti Human AMPK Alpha 2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2256
Datasheet
E WB
SDS
0.1 mg

Goat anti Human AMPK alpha 2 antibody recognizes human5′-AMP-activated protein kinase catalytic subunit alpha-2, also known as AMPK alpha 2, ACACA kinase, Acetyl-CoA carboxylase kinase or Hydroxymethylglutaryl-CoA reductase kinase, encoded by the PRKAA2 gene. AMPK alpha 2 is a a Serine/threonine-protein kinase and the catalytic subunit of the AMP-activated protein kinase (AMPK), a central metabolic sensor, essential for the regulation of fatty acid and cholesterol synthesis, in response to fluctuations in nutrient and intracellular energy levels.

AMPK has been implicated as a suppressor of cell proliferation of both normal and malignant cells. AMPK binding to the PS domain of ULK1, is essential for ULK1-mediated autophagy (Roach et al. 2011), and that the phosphorylation of raptor by AMPK, plays an important role in autophagy induction (Egan et al. 2011).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Bovine Expected from Sequence
Mouse
Rat
Pig Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human AMPK alpha 2 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence CPLDALNTTKP from the internal region of AMPK alpha 2 (NP_006243.2).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P54646
Entrez Gene
PRKAA2
GO Terms
GO:0005829 cytosol
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006112 energy reserve metabolic process
GO:0006468 protein phosphorylation
GO:0006633 fatty acid biosynthetic process
GO:0006695 cholesterol biosynthetic process
GO:0006853 carnitine shuttle
GO:0007050 cell cycle arrest
GO:0008286 insulin receptor signaling pathway
GO:0042304 regulation of fatty acid biosynthetic process
GO:0046320 regulation of fatty acid oxidation
Regulatory
For research purposes only

Applications of AMPK Alpha 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/16000
Western Blotting 1.0 3.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2256 detects a band of approximately 65kDa in Human heart and liver cell lysates.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

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