Alpha ACTININ-1 antibody

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Rabbit anti Alpha ACTININ-1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00889
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human alpha actinin-1 antibody recognizes alpha-actinin-1, also known as F-actin cross-linking protein, actinin 1 smooth muscle and alpha-actinin cytoskeletal isoform.

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN1 encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for ACTN1 (provided by RefSeq, Jul 2008).

Rabbit anti Human alpha actinin-1 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti alpha actinin-1 antibody recognizes a band of approximately 100 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Recombinant protein of human alpha actinin-1

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P12814
Entrez Gene
ACTN1
GO Terms
GO:0005509 calcium ion binding
GO:0002576 platelet degranulation
GO:0003779 actin binding
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005178 integrin binding
GO:0005730 nucleolus
GO:0005925 focal adhesion
GO:0015629 actin cytoskeleton
GO:0042981 regulation of apoptosis
GO:0017166 vinculin binding
GO:0030018 Z disc
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0031143 pseudopodium
GO:0048041 focal adhesion assembly
GO:0051271 negative regulation of cellular component movement
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Alpha ACTININ-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml