TNF Alpha antibody
Recombinant Dog TNF alpha (PDP018) detected using Goat anti Dog TNF alpha (AHP2975) as the capture reagent and biotinylated Goat anti Dog TNF alpha (AHP2975B) as the detection reagent followed by Streptavidin:HRP.
Goat anti Dog TNF alpha antibody recognizes canine tumor necrosis factor alpha (TNFα).
TNFα is produced by a range of cells including macrophages, monocytes, T and B cells, neutrophils and endothelial cells. TNFα has a key regulatory role in the canine immune response where it is involved in modulating the expression of numerous cytokines, growth factors, inflammatory mediators and acute phase proteins.
TNFα is produced by a range of cells including macrophages, monocytes, T and B cells, neutrophils and endothelial cells. TNFα has a key regulatory role in the canine immune response where it is involved in modulating the expression of numerous cytokines, growth factors, inflammatory mediators and acute phase proteins.
Product Details
- Target Species
- Dog
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to dog TNF alpha were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant dog tumor necrosis factor alpha (TNFα)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P51742
- Entrez Gene
- TNF
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- Regulatory
- For research purposes only
Applications of TNF Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.01ug/ml | 0.2ug/ml | |
| ELISA | 0.5ug/ml | 5ug/ml | |
| Western Blotting | 0.5ug/ml | 5ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used as a detection antibody in a sandwich ELISA together with AHP2975 as the capture reagent. Recombinant Dog TNF alpha (PDP018 ) may be used as a standard.
- ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with AHP2975B as the detection reagent. Recombinant Dog TNF alpha (PDP018 ) may be used as a standard.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
