TNF Alpha antibody

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Goat anti Dog TNF Alpha:Biotin

Goat anti Dog TNF Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2975B E 50 µg
AHP2975 E WB 0.1 mg
Goat anti Dog TNF alpha antibody recognizes canine tumor necrosis factor alpha (TNFα).

TNFα is produced by a range of cells including macrophages, monocytes, T and B cells, neutrophils and endothelial cells. TNFα has a key regulatory role in the canine immune response where it is involved in modulating the expression of numerous cytokines, growth factors, inflammatory mediators and acute phase proteins.

Product Details

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to dog TNF alpha were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant dog tumor necrosis factor alpha (TNFα)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch

More Information

UniProt
P51742 Related reagents
Entrez Gene
TNF Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
Regulatory
For research purposes only

Applications of TNF Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.01ug/ml 0.2ug/ml
ELISA 0.5ug/ml 5ug/ml
Western Blotting 0.5ug/ml 5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a detection antibody in a sandwich ELISA together with AHP2975 as the capture reagent. Recombinant Dog TNF alpha (PDP018 ) may be used as a standard.
ELISA
This product may be used as a capture antibody in a sandwich ELISA together with AHP2975B as the detection reagent. Recombinant Dog TNF alpha (PDP018 ) may be used as a standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB