TNF Alpha antibody

Goat anti Dog TNF Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
TNF Alpha

Product Code Applications Pack Size List Price Your Price Qty
AHP2975
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Goat anti Dog TNF alpha antibody recognizes canine tumor necrosis factor alpha (TNFα).

TNFα is produced by a range of cells including macrophages, monocytes, T and B cells, neutrophils and endothelial cells. TNFα has a key regulatory role in the canine immune response where it is involved in modulating the expression of numerous cytokines, growth factors, inflammatory mediators and acute phase proteins.

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to dog TNF alpha were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant dog tumor necrosis factor alpha (TNFα)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml 5ug/ml
Western Blotting 0.5ug/ml 5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a capture antibody in a sandwich ELISA together with AHP2975B as the detection reagent. Recombinant Dog TNF alpha (PDP018 ) may be used as a standard.

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Synonyms
TNFSF2
UniProt
P51742
Entrez Gene
TNF
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
View more products with TNF ALPHA specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Dog Products