IgG2 antibody

Sheep anti Dog IgG2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG2

Product Code Applications Pack Size List Price Your Price Qty
AHP948
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E WB 1 mg loader
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loader
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Sheep anti Dog IgG2 polyclonal antibody recognizes canine IgG2 by electrophoresis and ELISA. No cross reactivity was detected against other canine immunoglobulin classes, isolated immunoglobulin light chains or no immunoglobulin proteins.

Goat anti Dog IgG2 may cross react with IgG2 from other species.

Target Species
Dog
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to canine IgG2 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/10,000
Immunohistology - Frozen 1/200 1/2000
Western Blotting 1/1000 1/10,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for IgG2 antibody

  1. Bird, R.C. et al. (2011) An autologous dendritic cell canine mammary tumor hybrid-cell fusion vaccine.
    Cancer Immunol Immunother. 60 (1): 87-97.
  2. Agallou M et al. (2016) Identification of Immunoreactive Leishmania infantum Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis.
    PLoS One. 11 (2): e0149894.
  3. Martínez Abad, L.P. et al. (2017) Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis.
    Acta Trop. 166: 133-8.
  4. Khantavee, N. et al. (2020) Antibody levels to Malassezia pachydermatis. and Staphylococcus pseudintermedius. in atopic dogs and their relationship with lesion scores.
    Vet Dermatol. 31 (2): 111-115.

RRID
AB_567051
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Dog Products