IgG2 antibody

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Anti Dog IgG2 Antibody gallery image 1 — **Published customer image:**
Horseradish peroxidase conjugated Goat anti Canine IgG2 (**AHP948P**) antibody used to evaluate the immunoglobulin response to *Leishmania infantum* promastigotes by sympomatic and asymptomatic dogs by western blotting.
**Image caption:**
IgG reaction pattern associated with CVL status.
Total Leishmania infantum promastigote cell extracts were separated with 12% SDS-PAGE and electrotransferred to nitrocellulose membranes. The membranes were probed with serum obtained from individual asymptomatic dogs (1:500) or symptomatic dogs (1:1,000) and secondary anti-dog (A) IgG, (B) IgG1 or (C) IgG2 (1:10,000). Representative western blots from each group of dogs are depicted (asymptomatic: n = 5; symptomatic: n = 3). Numbers represent the code numbers of the dogs enrolled in the study. Molecular weight markers are shown on the left-hand side.

From: Agallou M, Athanasiou E, Samiotaki M, Panayotou G, Karagouni E (2016)
Identification of Immunoreactive Leishmania infantum Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis.
PLoS ONE 11(2): e0149894.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Sheep anti Dog IgG2

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: IgG2

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Secondary Antibodies
References (4)
Applications
Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

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AHP948 Datasheet Datasheet SDS SDS	C E WB	1 mg		Log in	Get Quote
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Sheep anti Dog IgG2 polyclonal antibody recognizes canine IgG2 by electrophoresis and ELISA. No cross reactivity was detected against other canine immunoglobulin classes, isolated immunoglobulin light chains or no immunoglobulin proteins.

Goat anti Dog IgG2 may cross react with IgG2 from other species.

Target Species: Dog
Product Form: Purified IgG - liquid
Antiserum Preparation: Antisera to canine IgG2 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/100	1/10,000
Immunohistology - Frozen	1/200	1/2000
Western Blotting	1/1000	1/10,000

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description	Product Code	Applications	Pack Size	Your Price
Rabbit anti Sheep IgG (H/L):Biotin	5184-2304	C E F WB	1.5 ml	Log in	Get Quote
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Description	Rabbit anti Sheep IgG (H/L):Biotin

References for IgG2 antibody

Bird, R.C. et al. (2011) An autologous dendritic cell canine mammary tumor hybrid-cell fusion vaccine.
Cancer Immunol Immunother. 60 (1): 87-97.
Agallou M et al. (2016) Identification of Immunoreactive Leishmania infantum Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis.
PLoS One. 11 (2): e0149894.
Martínez Abad, L.P. et al. (2017) Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis.
Acta Trop. 166: 133-8.
Khantavee, N. et al. (2020) Antibody levels to Malassezia pachydermatis. and Staphylococcus pseudintermedius. in atopic dogs and their relationship with lesion scores.
Vet Dermatol. 31 (2): 111-115.