TNF Alpha antibody

Rabbit anti Bovine TNF Alpha:Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
TNF Alpha

Product Code Applications Pack Size List Price Your Price Qty
AHP852Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.5 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti Bovine TNF alpha antibody recognizes bovine tumour necrosis factor alpha (TNF alpha).

TNF alpha is produced by a range of cells including macrophages, monocytes, T and B cells, neutrophils and endothelial cells. TNF alpha has a key regulatory role in the bovine immune response, where it is involved in modulating the expression of numerous cytokines, growth factors, inflammatory mediators and acute phase proteins.

Target Species
Bovine
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine TNF alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein G.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant bovine TNF alpha.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Endotoxin Level
<0.015 EU/ug
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1ug/ml 10ug/ml
Western Blotting 1ug/ml 10ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for TNF Alpha antibody

  1. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
    J Dairy Sci. 93: 2458-70.
  2. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
    Funct Integr Genomics. 10: 21-38.
  3. Cigliano, L. et al. (2016) Evaluation of serum markers of blood redox homeostasis and inflammation in PCB naturally contaminated heifers undergoing decontamination
    Science of The Total Environment. 542: 653-64.
  4. Canal AM et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection.
    Res Vet Sci. 110: 34-39.
  5. Baravalle ME et al. (2015) Altered Expression of Pro-inflammatory Cytokines in Ovarian Follicles of Cows with Cystic Ovarian Disease.
    J Comp Pathol. 153 (2-3): 116-30.
  6. Castel, A. et al. (2021) Recording and manipulation of vagus nerve electrical activity in chronically instrumented unanesthetized near term fetal sheep.
    J Neurosci Methods. 360: 109257.

RRID
AB_567236
UniProt
Q06599
Entrez Gene
TNF
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
View more products with TNF ALPHA specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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