Neurofilament H antibody
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Rabbit anti Bovine Neurofilament H 200 kDa antibody recognizes bovine intermediate 200 kDa Neurofilament H (NFH), a 1026 amino acid ~200 kDa protein involved in the maintenence of neuronal integrity.
Neurofilaments are a major component of the cellular cytoskeleton, acting as the most abundant support for the axon cytoplasm. They exist as three neurofilament proteins: 68/70 kDa (NFL) 160 kDa (NFM) and 200 kDa (NFH), which are expressed in both the central and peripheral nervous systems. Although typically expressed by neurons, neurofilaments are also expressed by neuroblastomas, neuromas, gangliogliomas, ganglioneuromas, and ganglioneuroblastomas, as well as paragangliomas, carcinoids, skin neuroendocrine carcinomas, and oat cell carcinomas of the lung (Lehto et al. 1983).
Defects in NFH are responsible for susceptibility to the neurodegenerative disorder amyotrophic lateral sclerosis (ALS) which affects upper and lower motor neurons, and results in fatal paralysis (Mendonça et al. 2005).
Neurofilaments are a major component of the cellular cytoskeleton, acting as the most abundant support for the axon cytoplasm. They exist as three neurofilament proteins: 68/70 kDa (NFL) 160 kDa (NFM) and 200 kDa (NFH), which are expressed in both the central and peripheral nervous systems. Although typically expressed by neurons, neurofilaments are also expressed by neuroblastomas, neuromas, gangliogliomas, ganglioneuromas, and ganglioneuroblastomas, as well as paragangliomas, carcinoids, skin neuroendocrine carcinomas, and oat cell carcinomas of the lung (Lehto et al. 1983).
Defects in NFH are responsible for susceptibility to the neurodegenerative disorder amyotrophic lateral sclerosis (ALS) which affects upper and lower motor neurons, and results in fatal paralysis (Mendonça et al. 2005).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Human Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to bovine NFH was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS-glycine buffered saline, NaCl
- Preservative Stabilisers
- 0.05% Sodium Azide (NaN3)
- Immunogen
- GST-tagged bovine NFH recombinant protein.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P12036
- P19246
- P16884
- Entrez Gene
- NEFH
- Nefh
- Nefh
- GO Terms
- GO:0005883 neurofilament
- GO:0007399 nervous system development
- GO:0008219 cell death
- GO:0000226 microtubule cytoskeleton organization
- GO:0005739 mitochondrion
- GO:0045110 intermediate filament bundle assembly
- GO:0060052 neurofilament cytoskeleton organization
- Regulatory
- For research purposes only
Applications of Neurofilament H antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 1.0ug/ml | ||
| Western Blotting | 0.1ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Bovine or rat cerebellum
- Western Blotting
- AHP2259GA detects a band of approximately 200kDa in bovine cerebellum tissue lysates.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Neurofilament H antibody
-
Kaneko, S. et al. (2006) Protecting axonal degeneration by increasing nicotinamide adenine dinucleotide levels in experimental autoimmune encephalomyelitis models.
J Neurosci. 26: 9794-804. -
Li, Y. et al. (2008) Transplanted olfactory ensheathing cells incorporated into the optic nerve head ensheathe retinal ganglion cell axons: possible relevance to glaucoma.
Neurosci Lett. 440: 251-4. -
Ignatov, A. et al. (2003) Role of the G-protein-coupled receptor GPR12 as high-affinity receptor for sphingosylphosphorylcholine and its expression and function in brain development.
J Neurosci. 23: 907-14. -
Leypoldt, F. et al. (2002) Neuronal differentiation of cultured human NTERA-2cl.D1 cells leads to increased expression of synapsins.
Neurosci Lett. 324 (1): 37-40. -
Savolainen, H. (2009) Cortical demyelination in PML and MS: similarities and differences.
Neurology. 72 (9): 861. -
Zhang, Z. et al. (2011) The role of single cell derived vascular resident endothelial progenitor cells in the enhancement of vascularization in scaffold-based skin regeneration.
Biomaterials. 32: 4109-17. -
Chen, K. et al. (2016) Differential Histopathological and Behavioral Outcomes Eight Weeks after Rat Spinal Cord Injury by Contusion, Dislocation, and Distraction Mechanisms.
J Neurotrauma. Apr 4. [Epub ahead of print]
Further Reading
-
Liu Q et al. (2004) Neurofilament proteins in neurodegenerative diseases.
Cell Mol Life Sci. 61 (24): 3057-75.
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