Neurofilament H antibody

Rabbit anti Bovine Neurofilament H 200kDa

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Neurofilament H
Region
200kDa

Product Code Applications Pack Size List Price Your Price Qty
AHP2259GA
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SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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Rabbit anti Bovine Neurofilament H 200 kDa antibody recognizes bovine intermediate 200 kDa Neurofilament H (NFH), a 1026 amino acid ~200 kDa protein involved in the maintenance of neuronal integrity.

Neurofilaments are a major component of the cellular cytoskeleton, acting as the most abundant support for the axon cytoplasm. They exist as three neurofilament proteins: 68/70 kDa (NFL) 160 kDa (NFM) and 200 kDa (NFH), which are expressed in both the central and peripheral nervous systems. Although typically expressed by neurons, neurofilaments are also expressed by neuroblastomas, neuromas, gangliogliomas, ganglioneuromas, and ganglioneuroblastomas, as well as paragangliomas, carcinoids, skin neuroendocrine carcinomas, and oat cell carcinomas of the lung (Lehto et al. 1983).

Defects in NFH are responsible for susceptibility to the neurodegenerative disorder amyotrophic lateral sclerosis (ALS) which affects upper and lower motor neurons, and results in fatal paralysis (Mendonça et al. 2005).

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Human
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to bovine NFH was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS-glycine buffered saline, NaCl
Preservative Stabilisers
0.05% Sodium Azide (NaN3)
Immunogen
GST-tagged bovine NFH recombinant protein.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1.0ug/ml
Western Blotting 0.1ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Bovine or rat cerebellum
Western Blotting
AHP2259GA detects a band of approximately 200kDa in bovine cerebellum tissue lysates.

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References for Neurofilament H antibody

  1. Kaneko, S. et al. (2006) Protecting axonal degeneration by increasing nicotinamide adenine dinucleotide levels in experimental autoimmune encephalomyelitis models.
    J Neurosci. 26: 9794-804.
  2. Li, Y. et al. (2008) Transplanted olfactory ensheathing cells incorporated into the optic nerve head ensheathe retinal ganglion cell axons: possible relevance to glaucoma.
    Neurosci Lett. 440: 251-4.
  3. Ignatov, A. et al. (2003) Role of the G-protein-coupled receptor GPR12 as high-affinity receptor for sphingosylphosphorylcholine and its expression and function in brain development.
    J Neurosci. 23: 907-14.
  4. Leypoldt, F. et al. (2002) Neuronal differentiation of cultured human NTERA-2cl.D1 cells leads to increased expression of synapsins.
    Neurosci Lett. 324 (1): 37-40.
  5. Savolainen, H. (2009) Cortical demyelination in PML and MS: similarities and differences.
    Neurology. 72 (9): 861.
  6. Zhang, Z. et al. (2011) The role of single cell derived vascular resident endothelial progenitor cells in the enhancement of vascularization in scaffold-based skin regeneration.
    Biomaterials. 32: 4109-17.
  7. Chen, K. et al. (2016) Differential Histopathological and Behavioral Outcomes Eight Weeks after Rat Spinal Cord Injury by Contusion, Dislocation, and Distraction Mechanisms.
    J Neurotrauma. 33 (18): 1667-84.

Further Reading

  1. Liu Q et al. (2004) Neurofilament proteins in neurodegenerative diseases.
    Cell Mol Life Sci. 61 (24): 3057-75.

Synonyms
Neurofilament Heavy Polypeptide
UniProt
P12036
P19246
P16884
Entrez Gene
NEFH
Nefh
Nefh
GO Terms
GO:0005883 neurofilament
GO:0007399 nervous system development
GO:0008219 cell death
GO:0000226 microtubule cytoskeleton organization
GO:0005739 mitochondrion
GO:0045110 intermediate filament bundle assembly
GO:0060052 neurofilament cytoskeleton organization
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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